Zebrafish: A Multifaceted Tool for Chemical Biologists

Basu, S.; Sachidanandan, Chetana

doi:10.1021/cr4000013

Cited by 56 publications

(45 citation statements)

References 357 publications

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“…To explore the potential of CPy‐Odot as an in vivo imaging probe, we applied confocal and time‐resolved fluorescence imaging measurements in living zebrafish 34. Briefly, the zebrafish was still alive after being injected with 300 nL CPy‐Odots into the ventricle.…”

Section: Resultsmentioning

confidence: 99%

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Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

et al. 2017

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The fluorophores with long‐lived fluorescent emission are highly desirable for time‐resolved fluorescence imaging (TRFI) in monitoring target fluorescence. By embedding the aggregates of a thermally activated delayed fluorescence (TADF) dye, 2,3,5,6‐tetracarbazole‐4‐cyano‐pyridine (CPy), in distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐poly(ethylene glycol) (DSPE‐PEG2000) matrix, CPy‐based organic dots (CPy‐Odots) with a long fluorescence lifetime of 9.3 μs (in water at ambient condition) and high brightness (with an absolute fluorescence quantum efficiency of 38.3%) are fabricated. CPy‐Odots are employed in time‐resolved and confocal fluorescence imaging in living Hela cells and in vivo. The green emission from the CPy‐Odots is readily differentiated from the cellular autofluorescence background because of their stronger emission intensities and longer lifetimes. Unlike other widely studied DSPE‐PEG2000 encapsulated Odots which are always distributed in cytoplasm, CPy‐Odots are located mainly in plasma membrane. In addition, the application of CPy‐Odots as a bright microangiography agent for TRFI in zebrafish is also demonstrated. Much broader application of CPy‐Odots is also prospected after further surface functionalization. Given its simplicity, high fluorescence intensity, and wide availability of TADF materials, the method can be extended to develop more excellent TADF Odots for accomplishing the challenges in future bioimaging applications.

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Section: Resultsmentioning

confidence: 99%

“…In Vivo Fluorescence Imaging : Zebrafish as animal model was purchased from Nanjing YSY Biotechnology Company Ltd. 300 nL CPy‐Odots (0.1 mg mL −1 ) was injected into ventricle using a reported procedure 34. The Zebrafish was anesthetized in 7% diethyl ether and transferred into 1.5% carboxymethyl cellulose sodium.…”

Section: Methodsmentioning

confidence: 99%

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

et al. 2017

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“…More recently, its versatility has also been recognized by chemists, which provides an opportunity to enhance interdisciplinary studies involving biology and chemistry [2] as in effect-directed analysis (EDA) [3].…”

Section: Introductionmentioning

confidence: 99%

The value of zebrafish as an integrative model in effect-directed analysis - a review

et al. 2015

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“…This whole animal model could potentially benefit several steps of the drug development processes, including drug screening, lead discovery, target identification, as well as functional assays (5)(6)(7). Recently, the zebrafish model has been used to analyze the dynamic course of leukocyte chemotactic migration in vivo, which facilitates novel insights into certain inflammatory diseases that involve leukocytes.…”

mentioning

confidence: 99%

Vibsanin B Preferentially Targets HSP90β, Inhibits Interstitial Leukocyte Migration, and Ameliorates Experimental Autoimmune Encephalomyelitis

Deng

Shao

et al. 2015

The Journal of Immunology

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Interstitial leukocyte migration plays a critical role in inflammation and offers a therapeutic target for treating inflammation-associated diseases such as multiple sclerosis. Identifying small molecules to inhibit undesired leukocyte migration provides promise for the treatment of these disorders. In this study, we identified vibsanin B, a novel macrocyclic diterpenoid isolated from Viburnum odoratissimum Ker-Gawl, that inhibited zebrafish interstitial leukocyte migration using a transgenic zebrafish line (TG:zlyz–enhanced GFP). We found that vibsanin B preferentially binds to heat shock protein (HSP)90β. At the molecular level, inactivation of HSP90 can mimic vibsanin B’s effect of inhibiting interstitial leukocyte migration. Furthermore, we demonstrated that vibsanin B ameliorates experimental autoimmune encephalomyelitis in mice with pathological manifestation of decreased leukocyte infiltration into their CNS. In summary, vibsanin B is a novel lead compound that preferentially targets HSP90β and inhibits interstitial leukocyte migration, offering a promising drug lead for treating inflammation-associated diseases.

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Zebrafish: A Multifaceted Tool for Chemical Biologists

Cited by 56 publications

References 357 publications

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

The value of zebrafish as an integrative model in effect-directed analysis - a review

Vibsanin B Preferentially Targets HSP90β, Inhibits Interstitial Leukocyte Migration, and Ameliorates Experimental Autoimmune Encephalomyelitis

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