Zebrafish: A smart tool for heart disease research

Zhang, Lantian; Zhou, Jinrun

doi:10.1111/jfb.15585

Cited by 4 publications

(2 citation statements)

References 151 publications

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“…[26]. Тем не менее в настоящее время в медико-биологических исследованиях лидерство занимают рыбки данио рерио (Danio rerio или Zebrafish) [54][55][56][57][58].…”

Section: аквариумные рыбки как инструмент для высокоточной температур...unclassified

Aquarium fish and temperature neuropharmacology: Update

Urakov,

Fisher,

Lebedev

et al. 2024

Psychopharmacology & biological narcology

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It is shown that at the end of the 20th century in Russia studies of temperature dependence of specific pharmacological activity of antihypoxants and some neurotropic drugs under normo- and hypothermia of various biological objects in experimental and clinical conditions were started. At the beginning of the 21st century, an original biological model of acute hypoxia in aquarium fish was developed. This model made it possible to start the study of fish resistance to hypoxia, to screen biologically active substances and to evaluate the activity of antihypoxants under normo- and hypothermia. The first results of using this model allowed to conclude that the dynamics of motor activity of fish under conditions of acute hypoxia is similar to the dynamics of motor activity of fetuses inside the uterus under diagnostic intrauterine hypoxia created by voluntary apnea in a pregnant woman. It was found that the value of the duration of the period of immobile state of fish and fetuses under hypoxia is directly proportional to the value of their resistance to hypoxia. It was found that the reduction in conditions of acute hypoxia water temperature with floating fish in it on 10 °C lengthens the duration of the period of immobile state of fish and the period of preservation of their viability more than 2 times, and the preliminary introduction of hydrogen peroxide in the water in the therapeutic dose in combination with hypothermia lengthens the corresponding periods by 4 times. It has also been found that such neurotropic drugs as ethyl alcohol, local and general anesthetics will completely eliminate the pain syndrome that develops during local hypothermia in normal. In addition, it has been shown that immersing the hands of people under alcohol intoxication and/or surgical anesthesia in water with melting ice for 2 minutes eliminates the feeling of pain in the hands and accelerates the development of subsequent hyperemia in the skin of the hands by 2 times compared to the norm.

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Section: аквариумные рыбки как инструмент для высокоточной температур...unclassified

Aquarium fish and temperature neuropharmacology: Update

Urakov,

Fisher,

Lebedev

et al. 2024

Psychopharmacology & biological narcology

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“…Zebrafish have been explored to study cardiac diseases (Gut et al, 2017;González-Rosa, 2022;Zhang and Zhou, 2023) as their heart show strikingly similar cardiac physiology to humans (Bakkers, 2011). Adult zebrafish models for human cardiomyopathies have been successfully generated (Ding et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Forward genetic screen using a gene-breaking trap approach identifies a novel role of grin2bb-associated RNA transcript (grin2bbART) in zebrafish heart function

Angom,

Joshi,

Patowary

et al. 2024

Front. Cell Dev. Biol.

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LncRNA-based control affects cardiac pathophysiologies like myocardial infarction, coronary artery disease, hypertrophy, and myotonic muscular dystrophy. This study used a gene-break transposon (GBT) to screen zebrafish (Danio rerio) for insertional mutagenesis. We identified three insertional mutants where the GBT captured a cardiac gene. One of the adult viable GBT mutants had bradycardia (heart arrhythmia) and enlarged cardiac chambers or hypertrophy; we named it “bigheart.” Bigheart mutant insertion maps to grin2bb or N-methyl D-aspartate receptor (NMDAR2B) gene intron 2 in reverse orientation. Rapid amplification of adjacent cDNA ends analysis suggested a new insertion site transcript in the intron 2 of grin2bb. Analysis of the RNA sequencing of wild-type zebrafish heart chambers revealed a possible new transcript at the insertion site. As this putative lncRNA transcript satisfies the canonical signatures, we called this transcript grin2bb associated RNA transcript (grin2bbART). Using in situ hybridization, we confirmed localized grin2bbART expression in the heart, central nervous system, and muscles in the developing embryos and wild-type adult zebrafish atrium and bulbus arteriosus. The bigheart mutant had reduced Grin2bbART expression. We showed that bigheart gene trap insertion excision reversed cardiac-specific arrhythmia and atrial hypertrophy and restored grin2bbART expression. Morpholino-mediated antisense downregulation of grin2bbART in wild-type zebrafish embryos mimicked bigheart mutants; this suggests grin2bbART is linked to bigheart. Cardiovascular tissues use Grin2bb as a calcium-permeable ion channel. Calcium imaging experiments performed on bigheart mutants indicated calcium mishandling in the heart. The bigheart cardiac transcriptome showed differential expression of calcium homeostasis, cardiac remodeling, and contraction genes. Western blot analysis highlighted Camk2d1 and Hdac1 overexpression. We propose that altered calcium activity due to disruption of grin2bbART, a putative lncRNA in bigheart, altered the Camk2d-Hdac pathway, causing heart arrhythmia and hypertrophy in zebrafish.

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Cardiac arrhythmia in individuals with steroid sulfatase deficiency (X-linked ichthyosis): candidate anatomical and biochemical pathways

Wren,

Davies

2024

Essays in Biochemistry

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Circulating steroids, including sex hormones, can affect cardiac development and function. In mammals, steroid sulfatase (STS) is the enzyme solely responsible for cleaving sulfate groups from various steroid molecules, thereby altering their activity and water solubility. Recent studies have indicated that Xp22.31 genetic deletions encompassing STS (associated with the rare dermatological condition X-linked ichthyosis), and common variants within the STS gene, are associated with a markedly elevated risk of cardiac arrhythmias, notably atrial fibrillation/flutter. Here, we consider emerging basic science and clinical findings which implicate structural heart abnormalities (notably septal defects) as a mediator of this heightened risk, and propose candidate cellular and biochemical mechanisms. Finally, we consider how the biological link between STS activity and heart structure/function might be investigated further and the clinical implications of work in this area.

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Zebrafish: A smart tool for heart disease research

Cited by 4 publications

References 151 publications

Aquarium fish and temperature neuropharmacology: Update

Aquarium fish and temperature neuropharmacology: Update

Forward genetic screen using a gene-breaking trap approach identifies a novel role of grin2bb-associated RNA transcript (grin2bbART) in zebrafish heart function

Cardiac arrhythmia in individuals with steroid sulfatase deficiency (X-linked ichthyosis): candidate anatomical and biochemical pathways

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