Zebrafish as a model for studying ovarian development: Recent advances from targeted gene knockout studies

Li, Jianzhen; Ge, Wei

doi:10.1016/j.mce.2020.110778

Cited by 59 publications

(37 citation statements)

References 274 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish ( D. rerio ) is one of the most common and beneficial vertebrate model organisms on which numerous biological developmental and disease studies have been carried out 8 , 9 . In addition to their use in fundamental studies, they were extensively employed in the fields of drug discovery, molecular toxicity, and therapeutics 10 – 12 .…”

Section: Introductionmentioning

confidence: 99%

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Subendran

Wang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

This study proposed a new experimental approach for the vascular and phenotype evaluation of the non-anesthetized zebrafish with representative imaging orientations for heart, pectoral fin beating, and vasculature views by means of the designed microfluidic device through inducing the optomotor response and hydrodynamic pressure control. In order to provide the visual cues for better positioning of zebrafish, computer-animated moving grids were generated by an in-house control interface which was powered by the larval optomotor response, in conjunction with the pressure suction control. The presented platform provided a comprehensive evaluation of internal circulation and the linked external behaviors of zebrafish in response to the cardiovascular parameter changes. The insights from these imaging sections was extended to identify the linkage between the cardiac parameters and behavioral endpoints. In addition, selected chemicals such as ethanol and caffeine were employed for the treatment of zebrafish. The obtained findings can be applicable for future investigation in behavioral drug screening serving as the forefront in psychopharmacological and cognition research.

show abstract

Section: Introductionmentioning

confidence: 99%

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Subendran

Wang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Early prophase, including leptotene and zygotene stages, are executed while oocytes develop in the germline cyst 10,21,27 , a conserved cellular organization of germ cells from insects to humans 28,29 . The germline cyst is generated during mitotic divisions of oocyte precursor cells called oogonia, whereby cytokinesis is incomplete, with cytoplasmic bridges (CBs) that persist between sister-cells and connect them 10,21,30,31 .…”

Section: Zygotene Cilia Extend Throughout the Germline Cystmentioning

confidence: 99%

Biomechanical control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

Mytils

Kumar

Qin³

et al. 2021

Preprint

View full text Add to dashboard Cite

Meiosis is a cellular program essential for the production of haploid gametes. A hallmark of meiosis is chromosomal pairing via synaptonemal complexes, and a major focus traditionally has been to understand synaptonemal complex formation. However, chromosomal pairing also depends on cytoplasmic counterparts that tether and rotate telomeres on the nuclear envelope, shuffling chromosomes and mechanically driving their homology searches. Rotating telomeres slide on perinuclear microtubules and are ultimately pulled towards the centrosome, forming a configuration called the zygotene chromosomal bouquet. The bouquet is universally conserved and is essential for pairing and fertility. However, despite its discovery in 1900, how the cytoplasmic counterparts of bouquet formation are mechanically regulated has remained enigmatic. Here, by studying zebrafish oogenesis, we report and comprehensively characterize a previously unrecognized cilium in oocytes, which we term the zygotene cilium. We show that the zygotene cilium specifically connects to the bouquet centrosome and constitutes a cable system of the cytoplasmic bouquet machinery. Farther, zygotene cilia extend throughout the germline cyst, a conserved cellular organization of germ cells. By analyzing multiple ciliary mutants, we demonstrate that the zygotene cilium is essential for chromosomal pairing, germ cell morphogenesis, ovarian development and fertility. We further show that the zygotene cilium is conserved in both male meiosis in zebrafish, as well as in mammalian oogenesis. Our work uncovers the novel concept of a cilium as a critical player in meiosis and sheds new light on reproduction phenotypes in ciliopathies. Furthermore, most cells in metazoans are ciliated and exhibit specific nuclear dynamics. We propose a cellular paradigm that cilia can control chromosomal dynamics.

show abstract

“…Therefore, efforts have been made over the last decades to dissect the complexity and behavior of different types of tumors, both at the in vitro and in vivo level, to potentiate the development of new and more effective therapeutics. Contrary to in vitro models, the main advantage of using living organisms is that tumor cells and the host directly interact, providing the opportunity to analyze both the tumor and its associated microenvironment, as well as cancer hallmarks, such as metastatic potential, angiogenesis or drug resistance [ 11 , 12 ]. Moreover, in vitro models have been shown to lack clinical predictive power due to the limitations associated with static 2D assays, such as the strong selection pressure associated with cell passaging [ 13 ].…”

Section: From the Problem To The Solution: Cancer And Personalizedmentioning

confidence: 99%

“…While Zebrafish are very popular aquarium fish, they are also key tools in many laboratories world-wide, with more than 800 biological laboratories using zebrafish as model animals to study different types of diseases [ 11 ]. Since the 1960s, zebrafish have traditionally been used as a model for studying developmental biology and vertebrate genetics [ 17 ].…”

Section: From the Problem To The Solution: Cancer And Personalizedmentioning

confidence: 99%

Modeling Cancer Using Zebrafish Xenografts: Drawbacks for Mimicking the Human Microenvironment

Cabezas-Sáinz

Pensado‐López

Sáinz

et al. 2020

Cells

View full text Add to dashboard Cite

The first steps towards establishing xenografts in zebrafish embryos were performed by Lee et al., 2005 and Haldi et al., 2006, paving the way for studying human cancers using this animal species. Since then, the xenograft technique has been improved in different ways, ranging from optimizing the best temperature for xenografted embryo incubation, testing different sites for injection of human tumor cells, and even developing tools to study how the host interacts with the injected cells. Nonetheless, a standard protocol for performing xenografts has not been adopted across laboratories, and further research on the temperature, microenvironment of the tumor or the cell–host interactions inside of the embryo during xenografting is still needed. As a consequence, current non-uniform conditions could be affecting experimental results in terms of cell proliferation, invasion, or metastasis; or even overestimating the effects of some chemotherapeutic drugs on xenografted cells. In this review, we highlight and raise awareness regarding the different aspects of xenografting that need to be improved in order to mimic, in a more efficient way, the human tumor microenvironment, resulting in more robust and accurate in vivo results.

show abstract

Zebrafish as a model for studying ovarian development: Recent advances from targeted gene knockout studies

Cited by 59 publications

References 274 publications

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Biomechanical control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

Modeling Cancer Using Zebrafish Xenografts: Drawbacks for Mimicking the Human Microenvironment

Contact Info

Product

Resources

About