Zebrafish as a Model for Germ Cell Regeneration

Cao, Zigang; Yang, Qifen; Luo, Lingfei

doi:10.3389/fcell.2021.685001

Cited by 6 publications

(2 citation statements)

References 67 publications

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“…For enrichment scores of axolotl regeneration-specific ATAC peaks (cluster 4 in Fig2C), fisher exact p-values was calculated. For the crossspecies motif analysis, ATAC-seq and RNA-seq data of zebrafish fin ( 22 ), zebrafish heart ( 23 ) and acoel ( 24 ) were processed and analyzed in the same way as axolotl; and MARA was performed to infer temporal motif activities.…”

Section: Methodsmentioning

confidence: 99%

Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration

Kawaguchi

Wang

Knapp³

et al. 2022

Preprint

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The salamander limb regenerates only the missing portion. Each limb segment can only form segments equivalent to- or more distal to their own identity, relying on a property termed, positional information. How positional information is encoded in limb cells has been unknown. By cell-type-specific chromatin profiling of upper arm, lower arm, and hand, we found segment-specific levels of histone H3K27me3 at limb homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment information code. During regeneration, regeneration-specific regulatory elements became active prior to the re-appearance of developmental regulatory elements. This means that, in the hand segment, the permissive chromatin state of the hand homeoprotein gene HoxA13 engages with regeneration regulatory elements, bypassing the upper limb program.

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Section: Methodsmentioning

confidence: 99%

Chromatin states at homeoprotein loci distinguish axolotl limb segments prior to regeneration

Kawaguchi

Wang

Knapp³

et al. 2022

Preprint

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“…7.1.2 | Mechanism 2: Lineage restriction (Figure 5b) Lineage restriction as a mechanism of regeneration or producing new tissues during posterior growth and asexual reproduction has been suggested in organisms such as axolotl, zebrafish, and mouse (Cao et al, 2019(Cao et al, , 2021Flowers et al, 2017;Johnson et al, 2020;Jopling et al, 2010;Kikuchi et al, 2010;Kragl et al, 2009;Lehoczky et al, 2011;Sanor et al, 2020). Two levels of lineage restriction can be considered: (1) restriction at the embryonic germ layer or (2) restriction at the cell type level.…”

Section: Models For Cellular Sources Of Germline Regenerationmentioning

confidence: 99%

Annelids as models of germ cell and gonad regeneration

Özpolat

2023

J Exp Zool Pt B

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Germ cells (reproductive cells and their progenitors) give rise to the next generation in sexually reproducing organisms. The loss or removal of germ cells often leads to sterility in established research organisms such as the fruit fly, nematodes, frog, and mouse. The failure to regenerate germ cells in these organisms reinforced the dogma of germline–soma barrier in which germ cells are set‐aside during embryogenesis and cannot be replaced by somatic cells. However, in stark contrast, many animals including segmented worms (annelids), hydrozoans, planaria, sea stars, sea urchins, and tunicates can regenerate germ cells. Here I review germ cell and gonad regeneration in annelids, a rich history of research that dates back to the early 20th century in this highly regenerative group. Examples include annelids from across the annelid phylogeny, across developmental stages, and reproductive strategies. Adult annelids regenerate germ cells as a part of regeneration, grafting, and asexual reproduction. Annelids can also recover germ cells after ablation of germ cell progenitors in the embryos. I present a framework to investigate cellular sources of germ cell regeneration in annelids, and discuss the literature that supports different possibilities within this framework, where germ–soma separation may or may not be preserved. With contemporary genetic‐lineage tracing and bioinformatics tools, and several genetically enabled annelid models, we are at the brink of answering the big questions that puzzled many for over more than a century.

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