Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis

Hu, Bo; Zhang, Wei; Feng, Xi; Ji, Wei; Xie, Xunwei; Xiao, Wuhan

doi:10.1016/j.ydbio.2014.01.005

Cited by 15 publications

(11 citation statements)

References 61 publications

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“…As a typical transcription factor, FOXO3, as well as its zebrafish homolog foxo3b, regulates the activity of other transcription factors through protein-protein interactions (34,35). In the present study, we found that foxo3b suppressed irf3/irf7 activity through interaction with irf3/irf7.…”

Section: Discussionsupporting

confidence: 57%

“…To further evaluate foxo3b loss of function on zebrafish antiviral response, we knocked down foxo3b in zebrafish embryos by injection of foxo3b-MO (34,35). Similar to those observed in foxo3b-null larvae, expressions of ifn1, mxc, and pkz were upregulated in the embryos with foxo3b-MO injection compared with the embryos with the STD-MO injection (Fig.…”

Section: Knockout Of Foxo3b In Zebrafish Enhances Antiviral Responsementioning

confidence: 64%

“…In zebrafish, there are two orthologs of the mammalian FOXO3 gene, termed foxo3a and foxo3b. Notably, zebrafish foxo3b, initially termed zFKHR/foxo5 (33), is more conserved to human FOXO3a, which shares 55% identity with human FOXO3a (34) and exhibits conserved function of mammalian FOXO3 (34,35).…”

Section: F Orkhead Box O (Foxo) Transcription Factors Homologsmentioning

confidence: 99%

“…Dominant-negative (DN) forms of irf3 and irf7 were cloned into pCMVMyc (Clontech) and pCMV-HA (Clontech) as described previously and subcloned into the psp64 vector (38). GFP and myc-tagged foxo3b in the psp64 vector were described previously (35). The standard morpholino control (STD-MO) and foxo3b splicing-blocking morpholino (foxo3b-MO) were described previously (34,35).…”

Section: Plasmid Construction and Reagentsmentioning

confidence: 99%

“…GFP and myc-tagged foxo3b in the psp64 vector were described previously (35). The standard morpholino control (STD-MO) and foxo3b splicing-blocking morpholino (foxo3b-MO) were described previously (34,35).…”

Section: Plasmid Construction and Reagentsmentioning

confidence: 99%

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Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Xing

Cai

Zhang

et al. 2016

The Journal of Immunology

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Forkhead box O (FOXO)3, a member of the FOXO family of transcription factors, plays key roles in various cellular processes, including development, longevity, reproduction, and metabolism. Recently, FOXO3 has also been shown to be involved in modulating the immune response. However, how FOXO3 regulates immunity and the underlying mechanisms are still largely unknown. In this study, we show that zebrafish (Danio rerio) foxo3b, an ortholog of mammalian FOXO3, is induced by polyinosinic-polycytidylic acid stimulation and spring viremia of carp virus (SVCV) infection. We found that foxo3b interacted with irf3 and irf7 to inhibit ifr3/irf7 transcriptional activity, thus resulting in suppression of SVCV or polyinosinic-polycytidylic acid–induced IFN activation. By suppressing expression of key antiviral genes, foxo3b negatively regulated the cellular antiviral response. Furthermore, upon SVCV infection, the expression of the key antiviral genes was significantly enhanced in foxo3b-null zebrafish larvae compared with wild-type larvae. Additionally, the replication of SVCV was inhibited in foxo3b-null zebrafish larvae, leading to a higher survival rate. Our findings suggest that by suppressing irf3/irf7 activity, zebrafish foxo3b negatively regulates the antiviral response, implicating the vital role of the FOXO gene family in innate immunity.

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Section: Discussionsupporting

confidence: 57%

Section: Knockout Of Foxo3b In Zebrafish Enhances Antiviral Responsementioning

confidence: 64%

Section: F Orkhead Box O (Foxo) Transcription Factors Homologsmentioning

confidence: 99%

Section: Plasmid Construction and Reagentsmentioning

confidence: 99%

Section: Plasmid Construction and Reagentsmentioning

confidence: 99%

See 3 more Smart Citations

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Xing

Cai

Zhang

et al. 2016

The Journal of Immunology

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Regulation of RNA polymerase II‐mediated transcriptional elongation: Implications in human disease

Sharma¹

2016

IUBMB Life

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Expression of protein-coding genes is primarily regulated at the level of transcription. Most of the earlier studies focussed on understanding the assembly of the pre-initiation complex at the promoter of genes and subsequent initiation of transcription as the regulatory steps in transcription. However, research over the last decade has demonstrated the significance of regulating transcription of genes at the elongation stage. Several new proteins have been identified that control this step and our knowledge about their functions is expanding rapidly. Moreover, an increasing body of evidence suggests that a dysfunction of these transcription elongation factors is related to several diseases. Here, we review the latest advances in our understanding about the in vivo roles of the transcription elongation factors and their link with diseases. V C 2016 IUBMB Life, 68(9): [709][710][711][712][713][714][715][716] 2016

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Arabidopsis thaliana possesses two novel ELL associated factor homologs

et al. 2021

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Transcription elongation is one of the key steps at which RNA polymerase II‐directed expression of protein‐coding genes is regulated in eukaryotic cells. Different proteins have been shown to control this process, including the ELL/EAF family. ELL Associated Factors (EAFs) were first discovered in a yeast two‐hybrid screen as interaction partners of the human ELL (Eleven nineteen Lysine‐rich Leukemia) transcription elongation factor. Subsequently, they have been identified in different organisms, including Schizosaccharomyces pombe. However, no homolog(s) of EAF has as yet been characterized from plants. In the present work, we identified EAF orthologous sequences in different plants and have characterized two novel Arabidopsis thaliana EAF homologs, AtEAF‐1 (At1g71080) and AtEAF‐2 (At5g38050). Sequence analysis showed that both AtEAF‐1 and AtEAF‐2 exhibit similarity with its S. pombe EAF counterpart. Moreover, both Arabidopsis thaliana and S. pombe EAF orthologs share conserved sequence characteristic features. Computational tools also predicted a high degree of disorder in regions towards the carboxyl terminus of these EAF proteins. We demonstrate that AtEAF‐2, but not AtEAF‐1 functionally complements growth deficiencies of Schizosaccharomyces pombe eaf mutant. We also show that only AtEAF‐1 displays transactivation potential resembling the S. pombe EAF ortholog. Subsequent expression analysis in A. thaliana showed that both homologs were expressed at varying levels during different developmental stages and in different tissues tested in the study. Individual null‐mutants of either AtEAF‐1 or AtEAF‐2 are developmentally normal implying their functional redundancy. Taken together, our results provide first evidence that A. thaliana also possesses functional EAF proteins, suggesting an evolutionary conservation of these proteins across organisms.

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Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis

Cited by 15 publications

References 61 publications

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Regulation of RNA polymerase II‐mediated transcriptional elongation: Implications in human disease

Arabidopsis thaliana possesses two novel ELL associated factor homologs

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