Zebrafish gbx1 refines the Midbrain-Hindbrain Boundary border and mediates the Wnt8 posteriorization signal

Rhinn, Muriel; Lun, Klaus; Ahrendt, Reiner; Geffarth, Michaela; Brand, Michael

doi:10.1186/1749-8104-4-12

Cited by 45 publications

(45 citation statements)

References 71 publications

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“…In contrast to mouse Gbx2, zebrafish gbx2 appears to be only involved in the maintenance of the anterior hindbrain (our results) and not in the establishment of the MHB (Kikuta et al, 2003;Rhinn et al, 2003Rhinn et al, , 2009). Therefore, it is possible that gbx2 is behaving as a survival factor in maintaining anterior hindbrain development.…”

Section: Gbx2 Knockdown Decreases Cell Proliferation and Increases Cecontrasting

confidence: 61%

“…Later it becomes restricted to a sharp transverse band at the isthmic region and is maintained in the anterior hindbrain in the developing neuraxis (Bouillet et al, 1995;Li and Joyner, 2001;Martinez-Barbera et al, 2001). Studies in zebrafish suggest that establishment of the MHB and positioning of the isthmic organizer is not a conserved role for gbx2 among vertebrate species (Kikuta et al, 2003;Rhinn et al, 2003Rhinn et al, , 2009. These studies show that gbx2 is not expressed early enough during development of the midbrain/hindbrain territory to accomplish the functions of Gbx2 in mouse.…”

Section: Introductionmentioning

confidence: 78%

“…In contrast, recent studies in zebrafish have shown that gbx1, and not gbx2, is required for initial positioning of the MHB and mediates posteriorization by Wnt8 signals. Moreover, based on temporal expression, gbx2 in zebrafish has been implicated in maintenance of the anterior hindbrain and not its specification (Kikuta et al, 2003;Rhinn et al, 2003Rhinn et al, , 2009. In this study, we determined whether gbx2/Gbx2 are functionally conserved in anterior hindbrain development or divergent as in MHB development.…”

Section: Discussionmentioning

confidence: 98%

“…In contrast to mouse, gbx1 and not gbx2 determines the position of the MHB and, consequently, placement of the isthmus in zebrafish (Rhinn et al, 2009). Therefore, to analyze the impact of gbx2 on anterior hindbrain development in zebrafish, it is vital to establish that MHB and gbx1 expression is not affected by a loss of gbx2 function.…”

Section: Early Expression Of Mhb Marker Fgf8 and Gbx1 Is Not Affectedmentioning

confidence: 99%

“…While Gbx2 is necessary for establishment of the MHB in mouse, zebrafish gbx1 and not gbx2 is required for MHB development (Kikuta et al, 2003;Rhinn et al, 2009). Interestingly, despite many studies in mice showing the necessity of Gbx2 for normal hindbrain development (Wassarman et al, 1997;Millet et al, 1999;Liu and Joyner, 2001b;Li et al, 2002;Waters and Lewandoski, 2006), few studies have focused on this role of Gbx2 in other vertebrate species.…”

Section: Introductionmentioning

confidence: 99%

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Evolutionarily conserved function of Gbx2 in anterior hindbrain development

et al. 2011

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The amino acid sequence across the DNA-binding homeodomain of Gbx2 is highly conserved across multiple species. In mice, Gbx2 is essential for establishment of the midbrain-hindbrain boundary (MHB), and in development of anterior hindbrain structures, rhombomeres (r) 1-r3, and the r2/r3-derived cranial nerve V. In contrast, studies in zebrafish have implicated gbx1 in establishment of the MHB. Therefore, we tested potential roles for gbx2 in anterior hindbrain development in zebrafish. gbx2 knockdown with antisense morpholino results in increased cell death in r2, r3, and r5 and a truncation of the anterior hindbrain, similar to the defect in Gbx2 2/2 mice. Moreover, there is abnormal clustering of cranial nerve V cell bodies in r2 and r3 indicative of defects in aspects of anterior hindbrain patterning. These phenotypes can be rescued by expression of the mouse GBX2 protein. These results suggest that gbx2/Gbx2 has an evolutionarily conserved role in anterior hindbrain development. Developmental Dynamics 240:828-838,

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Section: Gbx2 Knockdown Decreases Cell Proliferation and Increases Cecontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 98%

Section: Early Expression Of Mhb Marker Fgf8 and Gbx1 Is Not Affectedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolutionarily conserved function of Gbx2 in anterior hindbrain development

et al. 2011

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Development of the cerebellum and cerebellar neural circuits

Hibi

Shimizu

2012

Developmental Neurobiology

142

114

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The cerebellum, a structure derived from the dorsal part of the most anterior hindbrain, is important for integrating sensory perception and motor control. While the structure and development of the cerebellum have been analyzed most extensively in mammals,recent studies have shown that the anatomy and development of the cerebellum is conserved between mammals and bony fish (teleost) species, including zebrafish. In the mammalian and teleost cerebellum,Purkinje and granule cells serve, respectively, as the major GABAergic and glutamatergic neurons. Purkinje cells originate in the ventricular zone (VZ), and receive inputs from climbing fibers. Granule cells originate in the upper rhombic lip (URL) and receive inputs from mossy fibers. Thus, the teleost cerebellum shares many features with the cerebellum of other vertebrates, and isa good model system for studying cerebellar function and development. The teleost cerebellum also has features that are specific to teleosts or have not been elucidated in mammals, including eurydendroid cells and adult neurogenesis. Furthermore, the neural circuitry in part of the optic tectum and the dorsal hindbrain closely resembles the circuitry of the teleost cerebellum; hence,these are called cerebellum-like structures. Here we describe the anatomy and development of cerebellar neurons and their circuitry, and discuss the possible roles of the cerebellum and cerebellum-like structures in behavior and higher cognitive functions. We also consider the potential use of genetics and novel techniques for studying the cerebellum in zebrafish.

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Brain regionalization: Of signaling centers and boundaries

Cavodeassi

Houart

2012

Developmental Neurobiology

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Our knowledge of the general mechanisms controlling the formation of the vertebrate central nervous system has advanced tremendously in the last decade. Here, we discuss the impact of the combined use of cell manipulation, in vivo imaging and genetics in the zebrafish on recent progress in understanding how signaling processes progressively control regionalization of the central nervous system. We highlight the unresolved issues and speculate upon the fundamental role the zebrafish will continue having in answering them.

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Zebrafish gbx1 refines the Midbrain-Hindbrain Boundary border and mediates the Wnt8 posteriorization signal

Cited by 45 publications

References 71 publications

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

Development of the cerebellum and cerebellar neural circuits

Brain regionalization: Of signaling centers and boundaries

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