Zebrafish larva’s response and habituation to electric signal: Effects of voltage, current and pulsation studied in a microfluidic device

Khalili, Arezoo; Wijngaarden, Ellen van; Zoidl, Georg; Rezai, Pouya

doi:10.1016/j.sna.2021.113070

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…Particularly for spatial learning, the stimuli zones are located at different positions in the microfluidic devices to infuse the associated spatial information in the working organism. 3 Meanwhile, different types of positive 21,[33][34][35][36][37][38][39] and negative stimuli (motivators) [40][41][42][43][44][45] have been employed to investigate the associated behaviours of zebrafish larvae. A few positive stimuli, such as sight 33,34 and computer-based images, 35 have been employed to investigate the social behaviour of zebrafish larvae.…”

Section: Introductionmentioning

confidence: 99%

“…Other positive motivators, such as auditory cues 36,37 and appetitive reinforcement, 38 are reported to stimulate zebrafish in a simple associate learning paradigm. On the other hand, negative motivators such as optimal fear-inducing stimuli, 40 fish predators and their images, 41 optogenetics, 42 electric stimuli, 43,44 and chemical alarm substances 45 have been employed to investigate the fear and anxiety behaviours.…”

Section: Introductionmentioning

confidence: 99%

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Behavioural responses of zebrafish with sound stimuli in microfluidics

Loganathan

Chen

2023

Lab Chip

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Behavioural responses of zebrafish with sound stimuli in microfluidics

Loganathan

Chen

2023

Lab Chip

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“…We previously demonstrated visual and motor deficiencies in this fish line (Safarian et al, 2020). We also have reported different microfluidic techniques to study the electric‐induced behavioral responses of zebrafish larvae, quantitatively (Khalili et al, 2019, 2020; Khalili, van Wijngaarden, Youssef, et al, 2021; Khalili, van Wijngaarden, Zoidl, et al, 2021; Peimani et al, 2018). The lab‐on‐chip approach allows us to address the challenges of behavioral studies such as providing a controllable stimulus to evoke the behavioral responses of larvae and quantifying their quick movements.…”

Section: Introductionmentioning

confidence: 99%

Loss of Panx1 function in zebrafish alters motor behavior in a lab‐on‐chip model of Parkinson's disease

Khalili,

Safarian,

van Wijngaarden

et al. 2023

J of Neuroscience Research

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Pannexin 1 (Panx1) forms ATP‐permeable membrane channels that play roles in purinergic signaling in the nervous system. A link between Panx1 activity and neurodegenerative disorders including Parkinson's disease (PD) has been suggested, but experimental evidence is limited. Here, a zebrafish model of PD was produced by exposing panx1a+/+ and panx1a−/− zebrafish larvae to 6‐hydroxydopamine (6‐OHDA). Electrical stimulation in a microfluidic chip and quantitative real‐time‐qPCR of zebrafish larvae tested the role of Panx1 in both pathological and normal conditions. After 72‐h treatment with 6‐OHDA, the electric‐induced locomotor activity of 5 days post fertilization (5dpf) panx1a+/+ larvae were reduced, while the stimulus did not affect locomotor activity of age‐matched panx1a−/− larvae. A RT‐qPCR analysis showed an increase in the expression of genes that are functionally related to dopaminergic signaling, like the tyrosine hydroxylase (th2) and the leucine‐rich repeat kinase 2 (lrrk2). Extending the 6‐OHDA treatment duration to 120 h caused a significant reduction in the locomotor response of 7dpf panx1a−/− larvae compared to the untreated panx1a−/− group. The RT‐qPCR data showed a reduced expression of dopaminergic signaling genes in both genotypes. It was concluded that the absence of Panx1a channels compromised dopaminergic signaling in 6‐OHDA‐treated zebrafish larvae and that the increase in the expression of dopaminergic genes was transient, most likely due to a compensatory upregulation. We propose that zebrafish Panx1a models offer opportunities to shed light on PD's physiological and molecular basis. Panx1a might play a role on the progression of PD, and therefore deserves further investigation.

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Designing microfluidic devices for behavioral screening of multiple zebrafish larvae

Khalili

Wijngaarden

Youssef

et al. 2021

Biotechnology Journal

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Background Microfluidic devices are being used for phenotypic screening of zebrafish larvae in fundamental and pre‐clinical research. A challenge for the broad use of these microfluidic devices is their low throughput, especially in behavioral assays. Previously, we introduced the tail locomotion of a semi‐mobile zebrafish larva evoked on‐demand with electric signal in a microfluidic device. Here, we report the lessons learned for increasing the number of specimens from one to four larvae in this device. Methods and Results Multiple parameters including loading and testing time per fish and loading and orientation efficiencies were refined to optimize the performance of modified designs. Flow and electric field simulations within the final device provided insight into the flow behavior and functionality of traps when compared to previous single‐larva devices. Outcomes led to a new design which decreased the testing time per larva by ≈60%. Further, loading and orientation efficiencies increased by more than 80%. Critical behavioral parameters such as response duration and tail beat frequency were similar in both single and quadruple‐fish devices. Conclusion The developed microfluidic device has significant advantages for greater throughput and efficiency when behavioral phenotyping is required in various applications, including chemical testing in toxicology and gene screening.

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Zebrafish larva’s response and habituation to electric signal: Effects of voltage, current and pulsation studied in a microfluidic device

Cited by 5 publications

References 36 publications

Behavioural responses of zebrafish with sound stimuli in microfluidics

Behavioural responses of zebrafish with sound stimuli in microfluidics

Loss of Panx1 function in zebrafish alters motor behavior in a lab‐on‐chip model of Parkinson's disease

Designing microfluidic devices for behavioral screening of multiple zebrafish larvae

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