Zebrafish larvae show negative phototaxis to near-infrared light

Hartmann, Sarah; Vogt, Roland; Kunze, Jan; Rauschert, Anna; Kuhnert, Klaus-Dieter; Wanzenböck, Josef; Lamatsch, Dunja K.; Witte, Klaudia

doi:10.1371/journal.pone.0207264

Cited by 36 publications

(18 citation statements)

References 45 publications

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“…Our recording setup is dark-field illuminated via a custom made light-ring composed of 7 infrared (835 nm) emitting diodes (VSMY98545) that helped provide high-contrast images of both small prey particles and larval features. These are arranged appropriately such that illumination is uniformly distributed by the converging IR light beams onto the area of the circular 35 mm petridish, therefore eliminating light fluctuations and canceling any directional preferences arising from NIR light sources ( Hartmann et al, 2018 ). We provided a total of ≈250 mW to the light-ring and tried to keep power low to avoid any thermal currents in the water.…”

Section: Methodsmentioning

confidence: 99%

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Lagogiannis

Diana

Meyer

2020

eLife

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Goal-directed behaviours may be poorly coordinated in young animals but, with age and experience, behaviour progressively adapts to efficiently exploit the animal's ecological niche. How experience impinges on the developing neural circuits of behaviour is an open question. We have conducted a detailed study of the effects of experience on the ontogeny of hunting behaviour in larval zebrafish. We report that larvae with prior experience of live prey consume considerably more prey than naive larvae. This is mainly due to increased capture success and a modest increase in hunt rate. We demonstrate that the initial turn to prey and the final capture manoeuvre of the hunting sequence were jointly modified by experience and that modification of these components predicted capture success. Our findings establish an ethologically relevant paradigm in zebrafish for studying how the brain is shaped by experience to drive the ontogeny of efficient behaviour.

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Section: Methodsmentioning

confidence: 99%

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Lagogiannis

Diana

Meyer

2020

eLife

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show abstract

“…Our recording setup is dark-field illuminated via a custom made light-ring composed of 7 infrared (835nm) emitting diodes (VSMY98545) that helped provide high-contrast images of both small prey particles and larval features. These are arranged appropriately such that illumination is uniformly distributed by the converging IR light beams onto the area of the circular 35mm petridish, therefore eliminating light fluctuations and cancelling any directional preferences arising from NIR light sources [21]. We provided a total of ≈ 250mW to the light-ring and tried to keep power low to avoid any thermal currents in the water.…”

Section: B Behavioural Recordingmentioning

confidence: 99%

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Lagogiannis

Diana

Meyer

2019

Preprint

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The success of goal-directed behaviours relies on the coordinated execution of a sequence of component actions. In young animals, such sequences may be poorly coordinated, but with age and experience, behaviour progressively adapts to efficiently exploit the animal's ecological niche. How experience impinges on the developing neural circuits of behaviour is an open question. As a model system, larval zebrafish (Danio rerio) hold enormous potential for studying both the development of behaviour and the underlying circuits, but no relevant experience-dependent learning paradigm has yet been characterized. To address this, we have conducted a detailed study of the effects of experience on the ontogeny of hunting behaviour in larval zebrafish. We report that larvae with prior prey experience consume considerably more prey than naive larvae. This is mainly due to increased capture success that is also accompanied by a modest increase in hunt rate. We identified two components of the hunting sequence that are jointly modified by experience. At the onset of the hunting sequence, the orientation strategy of the turn towards prey is modified such that experienced larvae undershoot prey azimuth. Near the end of the hunt sequence, we find that experienced larvae are more likely to employ high-speed capture swims initiated from longer distances to prey. Combined, these modified turn and capture manoeuvrers can be used to predict the probability of capture success and suggest that their development provides advantages specific to larvae feeding on live-prey. Our findings establish an ethologically relevant paradigm in zebrafish for studying how the brain is shaped by experience to drive the ontogeny of efficient behaviour.

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“…Positive and negative phototaxis ( i.e ., towards or away from the light stimulus) is most often triggered by blue light detection, but not only ( Randel and Jekely, 2016 ). It may cover the whole spectrum, from UV/A up to near-infrared (Cyanobacteria, Chau et al, 2017 ; Wilde and Mullineaux, 2017 ) or just part of it (UV to green in the fruit fly Drosophila melanogaster larvae, Humberg and Sprecher, 2017 ); UV/blue in Hemiptera Diaphorina citri ( Paris et al, 2017 ); near-infrared in the zebrafish Danio rerio larvae ( Hartmann et al, 2018 ); and green in the bat Pipistrellus nathusii ( Voigt et al, 2017 ). Animals (particularly aquatic larvae) may change their preferences during development.…”

Section: Orientation In Space: Phototaxis Phototropismmentioning

confidence: 99%

Exposure to Artificial Light at Night and the Consequences for Flora, Fauna, and Ecosystems

et al. 2020

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The present review draws together wide-ranging studies performed over the last decades that catalogue the effects of artificial-light-at-night (ALAN) upon living species and their environment. We provide an overview of the tremendous variety of light-detection strategies which have evolved in living organisms - unicellular, plants and animals, covering chloroplasts (plants), and the plethora of ocular and extra-ocular organs (animals). We describe the visual pigments which permit photo-detection, paying attention to their spectral characteristics, which extend from the ultraviolet into infrared. We discuss how organisms use light information in a way crucial for their development, growth and survival: phototropism, phototaxis, photoperiodism, and synchronization of circadian clocks. These aspects are treated in depth, as their perturbation underlies much of the disruptive effects of ALAN. The review goes into detail on circadian networks in living organisms, since these fundamental features are of critical importance in regulating the interface between environment and body. Especially, hormonal synthesis and secretion are often under circadian and circannual control, hence perturbation of the clock will lead to hormonal imbalance. The review addresses how the ubiquitous introduction of light-emitting diode technology may exacerbate, or in some cases reduce, the generalized ever-increasing light pollution. Numerous examples are given of how widespread exposure to ALAN is perturbing many aspects of plant and animal behaviour and survival: foraging, orientation, migration, seasonal reproduction, colonization and more. We examine the potential problems at the level of individual species and populations and extend the debate to the consequences for ecosystems. We stress, through a few examples, the synergistic harmful effects resulting from the impacts of ALAN combined with other anthropogenic pressures, which often impact the neuroendocrine loops in vertebrates. The article concludes by debating how these anthropogenic changes could be mitigated by more reasonable use of available technology – for example by restricting illumination to more essential areas and hours, directing lighting to avoid wasteful radiation and selecting spectral emissions, to reduce impact on circadian clocks. We end by discussing how society should take into account the potentially major consequences that ALAN has on the natural world and the repercussions for ongoing human health and welfare.

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Zebrafish larvae show negative phototaxis to near-infrared light

Cited by 36 publications

References 45 publications

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Learning steers the ontogeny of an efficient hunting sequence in zebrafish larvae

Exposure to Artificial Light at Night and the Consequences for Flora, Fauna, and Ecosystems

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