Zebrafish Model for Screening Antiatherosclerosis Drugs

Han, Jichun; Zhang, Rui; Zhang, Xiaofeng; Dong, Jing; Chen, Ming‐Han; Pan, Yumin; Liao, Zi-Xian; Zhong, Min; He, Jingwen; Wang, Feiqiang; Yue, Yunyun; Shang, Jing

doi:10.1155/2021/9995401

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…Several studies showed that danio fish can be used to study the development of atherosclerosis and methods for its dietary prevention and treatment. The obtained data reveal that, with an appropriate diet (with a high cholesterol content), cholesterol and macrophages accumulate in blood vessels with an increase in the expression of inflammatory factors’ mRNA (V TNF-A and IL-6) and vascular cell adhesion molecules (vcam-1b), followed by the thickening of the intima of blood vessels ad the formation of plaques [ 263 ]. With a high cholesterol diet, hypercholesterolemia, oxidation of lipoproteins and the formation of fat bands in adult danio fish were observed [ 264 ].…”

Section: Resultsmentioning

confidence: 99%

Tackling Atherosclerosis via Selected Nutrition

Vesnina

Prosekov

Atuchin

et al. 2022

IJMS

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The development and pathogenesis of atherosclerosis are significantly influenced by lifestyle, particularly nutrition. The modern level of science and technology development promote personalized nutrition as an efficient preventive measure against atherosclerosis. In this survey, the factors were revealed that contribute to the formation of an individual approach to nutrition: genetic characteristics, the state of the microbiota of the gastrointestinal tract (GIT) and environmental factors (diets, bioactive components, cardioprotectors, etc.). In the course of the work, it was found that in order to analyze the predisposition to atherosclerosis associated with nutrition, genetic features affecting the metabolism of nutrients are significant. The genetic features include the presence of single nucleotide polymorphisms (SNP) of genes and epigenetic factors. The influence of telomere length on the pathogenesis of atherosclerosis and circadian rhythms was also considered. Relatively new is the study of the relationship between chrono-nutrition and the development of metabolic diseases. That is, to obtain the relationship between nutrition and atherosclerosis, a large number of genetic markers should be considered. In this relation, the question arises: “How many genetic features need to be analyzed in order to form a personalized diet for the consumer?” Basically, companies engaged in nutrigenetic research and choosing a diet for the prevention of a number of metabolic diseases use SNP analysis of genes that accounts for lipid metabolism, vitamins, the body’s antioxidant defense system, taste characteristics, etc. There is no set number of genetic markers. The main diets effective against the development of atherosclerosis were considered, and the most popular were the ketogenic, Mediterranean, and DASH-diets. The advantage of these diets is the content of foods with a low amount of carbohydrates, a high amount of vegetables, fruits and berries, as well as foods rich in antioxidants. However, due to the restrictions associated with climatic, geographical, material features, these diets are not available for a number of consumers. The way out is the use of functional products, dietary supplements. In this approach, the promising biologically active substances (BAS) that exhibit anti-atherosclerotic potential are: baicalin, resveratrol, curcumin, quercetin and other plant metabolites. Among the substances, those of animal origin are popular: squalene, coenzyme Q10, omega-3. For the prevention of atherosclerosis through personalized nutrition, it is necessary to analyze the genetic characteristics (SNP) associated with the metabolism of nutrients, to assess the state of the microbiota of the GIT. Based on the data obtained and food preferences, as well as the individual capabilities of the consumer, the optimal diet can be selected. It is topical to exclude nutrients of which their excess consumption stimulates the occurrence and pathogenesis of atherosclerosis and to enrich the diet with functional foods (FF), BAS containing the necessary anti-atherosclerotic, and stimulating microbiota of the GIT nutrients. Personalized nutrition is a topical preventive measure and there are a number of problems hindering the active use of this approach among consumers. The key factors include weak evidence of the influence of a number of genetic features, the high cost of the approach, and difficulties in the interpretation of the results. Eliminating these deficiencies will contribute to the maintenance of a healthy state of the population through nutrition.

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Section: Resultsmentioning

confidence: 99%

Tackling Atherosclerosis via Selected Nutrition

Vesnina

Prosekov

Atuchin

et al. 2022

IJMS

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“…Increased severity when induced with both high cholesterol diet and lipopolysaccharide compared to individual treatment. [ 150 ] Zebrafish model of Atherosclerosis Zebrafish fed with high cholesterol diet Investigate the protective activity of Dendrobium huoshanense Polysaccharide Treatment of polysaccharide decreased plaque formation, neutrophil recruitment, and levels of total cholesterol in high cholesterol diet induced larvae. [ 151 ] Zebrafish model of cardiovascular disease HEG1 knockout zebrafish using CRISPR-Cas9 Study the mechanism and pathophysiology of cardiovascular diseases.…”

Section: Zebrafish Models To Investigate Therapeutics Against Chronic...mentioning

confidence: 99%

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

Balde,

Ramya,

Nazeer

2024

Heliyon

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“…Considering that the cardiovascular system of zebrafish (Danio rerio) exhibits similarity to that of mammals in both anatomical structure and physiological function (Konstantin Stoletov et al 2009 ; Jichun Bahrami and Childs 2020 ; Han et al 2021 ), zebrafish have become extensively employed in cardiovascular disease research (Peterson and Macrae 2012 ; Philipp Gut et al 2017 ). The three-layered arterial structure, consisting of an intima, a midmembrane, and an epithelium, is similar to that of humans (Marie Hoareau et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Inducing aortic aneurysm/dissection in zebrafish: evaluating the efficacy of β-Aminopropionic Nitrile as a model

Zhang,

Liang,

Zeng

et al. 2024

Animal Cells and Systems

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Aortic aneurysm/dissection (AAD) poses a life-threatening cardiovascular emergency with complex mechanisms and a notably high mortality rate. Zebrafish (Danio rerio) serve as valuable models for AAD due to the conservation of their three-layered arterial structure and genome with that of humans. However, the existing studies have predominantly focused on larval zebrafish, leaving a gap in our understanding of adult zebrafish. In this study, we utilized β-Aminopropionic Nitrile (BAPN) impregnation to induce AAD in both larval and adult zebrafish. Following induction, larval zebrafish exhibited a 28% widening of the dorsal aortic diameter ( p < 0.0004, n = 10) and aortic arch malformations, with a high malformation rate of 75% (6/8). Conversely, adult zebrafish showed a 41.67% (5/12) mortality rate 22 days post-induction. At this time point, the dorsal aortic area had expanded by 2.46 times ( p < 0.009), and the vessel wall demonstrated significant thickening (8.22 ± 2.23 μM vs. 26.38 ± 10.74 μM, p < 0.05). Pathological analysis revealed disruptions in the smooth muscle layer, contributing to a 58.33% aneurysm rate. Moreover, the expression levels of acta2, tagln, cnn1a, and cnn1b were decreased, indicating a weakened contractile phenotype. Transcriptome sequencing showed a significant overlap between the molecular features of zebrafish tissues post-BAPN treatment and those of AAD patients. Our findings present a straightforward and practical method for generating AAD models in both larval and adult zebrafish using BAPN.

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Zebrafish Model for Screening Antiatherosclerosis Drugs

Cited by 7 publications

References 32 publications

Tackling Atherosclerosis via Selected Nutrition

Tackling Atherosclerosis via Selected Nutrition

A review on current advancement in zebrafish models to study chronic inflammatory diseases and their therapeutic targets

Inducing aortic aneurysm/dissection in zebrafish: evaluating the efficacy of β-Aminopropionic Nitrile as a model

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