Zebrafish models to study drug abuse-related phenotypes

Stewart, Adam; Wong, Keith; Cachat, Jonathan; Gaikwad, Siddharth; Kyzar, Evan J.; Wu, Nadine; Hart, Peter C.; Piet, Valerie; Utterback, Eli; Elegante, Marco; Tien, David; Kalueff, Allan V.

doi:10.1515/rns.2011.011

Cited by 129 publications

(81 citation statements)

References 150 publications

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“…have the potential to foster further high-throughput drug discovery, neurodevelopmental studies, and substance abuse research using zebrafish. 46,86 In summary, this report outlines the rationale, current successes, and conceptual framework for developing novel efficient models of hallucinogenic drug action in zebrafish. The growing utility of such aquatic models to study hallucinogen-induced phenotypes will foster future experimental studies in this field, markedly enhancing both mechanistically driven top-down translational research, as well as bottom-up high-throughput screening for novel biomarkers and drug targets.…”

Section: ■ Concluding Remarksmentioning

confidence: 99%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

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Section: ■ Concluding Remarksmentioning

confidence: 99%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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“…Furthermore, with the aim of generating highthroughput behavioural data [5][6][7], considerable research is being performed to integrate computer-animated images and robotic replicas of conspecifics, heterospecifics and predators [8][9][10][11][12]. Zebrafish have been used to study the fundamental mechanisms governing the exhibition of emotional patterns [13,14], individual response to alcohol and drugs of abuse [15][16][17], and higher order brain functions, such as memory and learning [18]. Zebrafish behaviour is typically measured from locomotory patterns [19,20], which are detected in the form of changes in speed and turn rate [5,6]; these patterns are annotated via human observation [21] or classified on the basis of sequences of trajectory data obtained using vision-based tracking [22,23].…”

Section: Introductionmentioning

confidence: 99%

A jump persistent turning walker to model zebrafish locomotion

Mwaffo

Anderson

Butail

et al. 2015

J. R. Soc. Interface.

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Zebrafish are gaining momentum as a laboratory animal species for the investigation of several functional and dysfunctional biological processes. Mathematical models of zebrafish behaviour are expected to considerably aid in the design of hypothesis-driven studies by enabling preliminary in silico tests that can be used to infer possible experimental outcomes without the use of zebrafish. This study is motivated by observations of sudden, drastic changes in zebrafish locomotion in the form of large deviations in turn rate. We demonstrate that such deviations can be captured through a stochastic mean reverting jump diffusion model, a process that is commonly used in financial engineering to describe large changes in the price of an asset. The jump process-based model is validated on trajectory data of adult subjects swimming in a shallow circular tank obtained from an overhead camera. Through statistical comparison of the empirical distribution of the turn rate against theoretical predictions, we demonstrate the feasibility of describing zebrafish as a jump persistent turning walker. The critical role of the jump term is assessed through comparison with a simplified mean reversion diffusion model, which does not allow for describing the heavy-tailed distributions observed in the fish turn rate.

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“…1 The zebrafish has been shown to be a viable model for the study of complex behaviors and addiction. [2][3][4][5] These behavioral assays have been used in hopes of better defining and/or developing new treatments for the disorders being modeled in each study. In the design of a behavioral assay, the control of underlying variability in the individuals used in the study is important as these differences may confound or mask the measurement of a true signal.…”

Section: Introductionmentioning

confidence: 99%

Reducing the Noise in Behavioral Assays: Sex and Age in Adult Zebrafish Locomotion

Philpott¹,

Donack²,

Cousin

et al. 2012

Zebrafish

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Many assays are used in animal model systems to measure specific human disease-related behaviors. The use of both adult and larval zebrafish as a behavioral model is gaining popularity. As this work progresses and potentially translates into new treatments, we must do our best to improve the sensitivity of these assays by reducing confounding factors. Scientists who use the mouse model system have demonstrated that sex and age can influence a number of behaviors. As a community, they have moved to report the age and sex of all animals used in their studies. Zebrafish work does not yet carry the same mandate. In this study, we evaluated sex and age differences in locomotion behavior. We found that age was a significant factor in locomotion, as was sex within a given age group. In short, as zebrafish age, they appear to show less base level locomotion. With regard to sex, younger (10 months) zebrafish showed more locomotion in males, while older zebrafish (22 months) showed more movement in females. These findings have led us to suggest that those using the zebrafish for behavioral studies control for age and sex within their experimental design and report these descriptors in their methods.

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Zebrafish models to study drug abuse-related phenotypes

Cited by 129 publications

References 150 publications

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

A jump persistent turning walker to model zebrafish locomotion

Reducing the Noise in Behavioral Assays: Sex and Age in Adult Zebrafish Locomotion

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