Zebrafish R‐cadherin (Cdh4) controls visual system development and differentiation

Babb, Sherry G.; Kotradi, Shannon M.; Shah, Bijal; Chiappini-Williamson, C.; Bell, Lauren N.; Schmeiser, Glen; Chen, Elbert; Liu, Qin; Marrs, James A.

doi:10.1002/dvdy.20431

Cited by 49 publications

(82 citation statements)

References 51 publications

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“…These embryos were similar to the moderately to severely affected embryos shown in our previous study (Babb et al, 2005). Moreover, zn5 (an antibody that labels differentiating neurons and their processes) or acetylated tubulin antibody (that labels ␣-tubulin) immunostaining demonstrated that these embryos had a much reduced retinal ganglion cell layer and retinal axons, as reported in our study of Cdh4 function in zebrafish visual system development (Babb et al, 2005). Embryos injected with lower concentrations of the MOs (0.125 mM, 1.0 ng/embryo for RcadMphA, 0.06 mM, 0.5 ng/embryo for RcadMphB) were largely indistinguishable from control embryos.…”

Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralsupporting

confidence: 89%

“…By 50 -55 hpf, the majority of the cdh4 morphants (716/867, 86.6%) showed a similar gross morphology to the control embryos, except that the cdh4 morphants had eyes of reduced size, although some were only slightly smaller, and some had slightly ventrally curled tails. These embryos were similar to the moderately to severely affected embryos shown in our previous study (Babb et al, 2005). Moreover, zn5 (an antibody that labels differentiating neurons and their processes) or acetylated tubulin antibody (that labels ␣-tubulin) immunostaining demonstrated that these embryos had a much reduced retinal ganglion cell layer and retinal axons, as reported in our study of Cdh4 function in zebrafish visual system development (Babb et al, 2005).…”

Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralsupporting

confidence: 88%

“…Large variations in the number of TUNEL-positive cells in the ganglia of both control embryos and cdh4 morphants likely resulted from the small sizes of the cranial and lateral line ganglia. Increased cell death was also detected in the cdh4 morphant retina (Babb et al, 2005).…”

Section: Fig 1 Cdh4 Immunostaining Showing Thatmentioning

confidence: 86%

“…Injection of cdh4-specific antisense morpholino oligonucleotides (cdh4 MOs: RcadMphA, 0.25 mM, 2.1 ng/embryo, or RcadMphB, 0.12 mM, 1.0 ng/embryo), into one-to four-cell stage zebrafish embryos greatly reduced Cdh4 protein levels in the injected embryos at 50 -55 hpf ( Fig. 1; also see Babb et al, 2005). At 24 hr post fertilization (hpf), the injected embryos (cdh4 morphants) were similar in body shape and size to control (uninjected) embryos.…”

Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralmentioning

confidence: 93%

“…Cadherin-4 (Cdh4), also called R-cadherin, is a member of the type-I classical cadherin subfamily (Redies, 1995;Nollet et al, 2000). Cdh4 expression and/or function in the vertebrate nervous system has been studied in a variety of vertebrate species including zebrafish, Xenopus, chicken, and mouse (Inuzuka et al, 1991;Redies et al, 1992;Ganzler and Redies, 1995;Matsunami and Takeichi, 1995;Tashiro et al, 1995;Liu et al, 1999;Wohrn et al, 1998;Gerhardt et al, 2000;Honjo et al, 2000;Treubert-Zimmermann et al, 2002;Andrews and Mastick, 2003;Babb et al, 2005). We previously showed that Cdh4/cdh4 is expressed in the majority of the cranial and lateral line ganglia and their nerves in the developing zebrafish (Liu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Cadherin‐4 plays a role in the development of zebrafish cranial ganglia and lateral line system

Wilson

Shen

Clendenon

et al. 2007

Developmental Dynamics

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We previously reported that cadherin-4 (also called R-cadherin) was expressed by the majority of the developing zebrafish cranial and lateral line ganglia. Cadherin-4 (Cdh4) function in the formation of these structures in zebrafish was studied using morpholino antisense technology. Differentiation of the cranial and lateral line ganglia and lateral line nerve and neuromasts of the cdh4 morphants was analyzed using multiple neural markers. We found that a subset of the morphant cranial and lateral line ganglia were disorganized, smaller, with reduced staining, and/or with altered shape compared to control embryos. Increased cell death in the morphant ganglia likely contributed to these defects. Moreover, cdh4 morphants had shorter lateral line nerves and a reduced number of neuromasts, which was likely caused by disrupted migration of the lateral line primordia. These results indicate that Cdh4 plays a role in the normal formation of the zebrafish lateral line system and a subset of the cranial ganglia. Developmental Dynamics 236:893-902, 2007.

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Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralsupporting

confidence: 89%

Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralsupporting

confidence: 88%

Section: Fig 1 Cdh4 Immunostaining Showing Thatmentioning

confidence: 86%

Section: Cdh4 Is Involved In the Formation Of The Cranial And Lateralmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cadherin‐4 plays a role in the development of zebrafish cranial ganglia and lateral line system

Wilson

Shen

Clendenon

et al. 2007

Developmental Dynamics

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Novel distribution of junctional adhesion molecule‐C in the neural retina and retinal pigment epithelium

Daniele

Adams

Durante

et al. 2007

J of Comparative Neurology

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Junction adhesion molecules-A, -B, and -C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam-C mRNA was increased threefold in the all-cone retina of the Nrl(-/-) mouse, suggesting that Jam-C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam-C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between Müller and photoreceptor cells. Additionally, Jam-C labeling was observed in the long apical processes of Müller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam-B was also detected at the OLM. In the developing retina, Jam-B and -C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam-C(-/-) mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam-A was not detected at the OLM in wild-type retinas, it was present at the OLM in retinas of Jam-C(-/-) mice. These findings indicate that up-regulation of Jam-A in the retina compensates for the loss of Jam-C. The nonclassical distribution of Jam-C in the apical membranes of Müller cells and RPE suggests that Jam-C has a novel function in the retina.

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Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration

Ramachandran

Reifler

Parent

et al. 2010

J of Comparative Neurology

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The tuba1a gene encodes a neural-specific alpha-tubulin isoform whose expression is restricted to the developing and regenerating nervous system. Using zebrafish as a model system for studying CNS regeneration we recently showed that retinal injury induces tuba1a gene expression in Müller glia that reentered the cell cycle. However, due to the transient nature of tuba1a gene expression during development and regeneration, it was not possible to trace the lineage of the tuba1a-expressing cells with a reporter directly under the control of the tuba1a promoter. To overcome this limitation, we generated tuba1a:CreER T2 and β-actin2:loxP-mCherrry-loxP-GFP double transgenic fish that allowed us to conditionally and permanently label tuba1a-expressing cells via ligand-induced recombination. During development, recombination revealed transient tuba1a expression in not only neural progenitors, but also cells that contribute to skeletal muscle, heart and intestine. In the adult, recombination revealed tuba1a expression in brain, olfactory neurons and sensory cells of the lateral line, but not in the retina. Following retinal injury, recombination showed tuba1a expression in Müller glia that had reentered the cell cycle and lineage tracing indicated these cells are responsible for regenerating retinal neurons and glia. These results suggest that tuba1a-expressing progenitors contribute to multiple cell lineages during development and that tuba1a-expressing Müller glia are retinal progenitors in the adult.

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Zebrafish R‐cadherin (Cdh4) controls visual system development and differentiation

Cited by 49 publications

References 51 publications

Cadherin‐4 plays a role in the development of zebrafish cranial ganglia and lateral line system

Cadherin‐4 plays a role in the development of zebrafish cranial ganglia and lateral line system

Novel distribution of junctional adhesion molecule‐C in the neural retina and retinal pigment epithelium

Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration

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