Zebrafish swimming behavior as a biomarker for ototoxicity-induced hair cell damage: a high-throughput drug development platform targeting hearing loss

Niihori, Maki; Platto, Terry; Igarashi, Suzu; Hurbon, Audriana N.; Dunn, Anita M.; Tran, Phi; Tran, Hung C.; Mudery, Jordan A.; Slepian, Marvin J.; Jacob, Abraham

doi:10.1016/j.trsl.2015.05.002

Cited by 31 publications

(24 citation statements)

References 26 publications

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“…The presence of silent cells suggests that not all cells are needed for proper lateral-line function and that silent cells exist as backups. In support of this, previous work found that after moderate damage of hair cells in the lateral line, no quantifiable behavioral deficit was observed 58 . Therefore, anatomical redundancy and presynaptic silencing may work together to create a sensory system that functions with the minimum number of cells and connections.…”

Section: Discussionsupporting

confidence: 67%

Synaptically silent sensory hair cells in zebrafish are recruited after damage

et al. 2018

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Analysis of mechanotransduction among ensembles of sensory hair cells in vivo is challenging in many species. To overcome this challenge, we used optical indicators to investigate mechanotransduction among collections of hair cells in intact zebrafish. Our imaging reveals a previously undiscovered disconnect between hair-cell mechanosensation and synaptic transmission. We show that saturating mechanical stimuli able to open mechanically gated channels are unexpectedly insufficient to evoke vesicle fusion in the majority of hair cells. Although synaptically silent, latent hair cells can be rapidly recruited after damage, demonstrating that they are synaptically competent. Therefore synaptically silent hair cells may be an important reserve that acts to maintain sensory function. Our results demonstrate a previously unidentified level of complexity in sculpting sensory transmission from the periphery.

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Section: Discussionsupporting

confidence: 67%

Synaptically silent sensory hair cells in zebrafish are recruited after damage

et al. 2018

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“…112 Secondly, higher concentrations of cisplatin may affect other systems of zebrafish (e.g., neurotransmitters or motor neurons), changing their swimming behavior. 6 While collecting data for the present review, we have identified four articles dedicated to adult zebrafish inner ear (⩾6 mpf) and cisplatin-induced ototoxicity that were published between 2009 and 2020. Two of the manuscripts were studying the curcuminoids-dependent otoprotection against cisplatin.…”

Section: Discussionmentioning

confidence: 99%

Use of zebrafish larvae lateral line to study protection against cisplatin-induced ototoxicity: A scoping review

Domarecka

Skarzynska

Szczepek

et al. 2020

Int J Immunopathol Pharmacol

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Aim: The present review aimed to consolidate and analyze the recent information about the use of zebrafish in studies concerning cisplatin-induced ototoxicity and otoprotection. Material and methods: The PubMed, Web of Science, and Scopus databanks were searched using the following MESH terms: zebrafish, cisplatin, ototoxicity. The identified publications were screened according to inclusion and exclusion criteria and the 26 qualifying manuscripts were included in the full-text analysis. The experimental protocols, including cisplatin concentrations, the exposure duration and the outcome measurements used in zebrafish larvae studies, were evaluated and the reported knowledge was summarized. Results: Twenty-six substances protecting from cisplatin-induced toxicity were identified with the use of zebrafish larvae. These substances include quinine, salvianolic acid B, berbamine 6, benzamil, quercetin, dexmedetomidine, dexamethsanone, quinoxaline, edaravone, apocynin, dimethyl sulfoxide, KR-22335, SRT1720, ORC-13661, 3-MA, D-methionine, mdivi-1, FUT-175, rapamycin, Z-LLF-CHO, ATX, NAC, CYM-5478, CHCP1, CHCP2 and leupeptin. The otoprotective effects of compounds were attributed to their anti-ROS, anti-apoptotic and cisplatin uptake-blocking properties. The broadest range of protection was achieved when the experimental flow used preconditioning with an otoprotective compound and later a co-incubation with cisplatin. Protection against a high concentration of cisplatin was observed only in protocols using short exposure times (4 and 6 h). Conclusions: The data extracted from the selected papers confirm that despite the differences between the human and the zebra fish hearing thresholds (as affected by cisplatin), the sensory cells of zebrafish and larval zebrafish are a valuable tool which could be used: (i) for the discovery of novel otoprotective substances and compounds; (ii) to screen their side effects and (iii) to extend the knowledge on the mechanisms of cisplatin-induced inner ear damage. For future studies, the development of a consensus experimental protocol is highly recommended.

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“…However, this desynchronization was not observed. The concern on interanimal interference can also be further evaluated by using infrared illumination during the experiment [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses

et al. 2017

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The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. The classical approach to examine hair cells is to use dye to conduct selective staining, which shows the number and morphology of hair cells but does not reveal their function. Startle response is a behavior closely related to the auditory function of hair cells; therefore it can be used to measure the function of hair cells. In this study, we developed a device to measure the startle response of zebrafish larvae. By applying various levels of stimulus, it showed that the system can discern a 10 dB difference. The hair cell in zebrafish can regenerate after damage due to noise exposure or drug treatment. With this device, we measured the startle response of zebrafish larvae during and after drug treatment. The results show a similar trend to the classical hair cell staining method. The startle response was reduced with drug treatment and recovered after removal of the drug. Together it demonstrated the capability of this behavioral assay in evaluating the hair cell functions of fish larvae and its potential as a high-throughput screening tool for auditory-related gene and drug discovery.

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Zebrafish swimming behavior as a biomarker for ototoxicity-induced hair cell damage: a high-throughput drug development platform targeting hearing loss

Cited by 31 publications

References 26 publications

Synaptically silent sensory hair cells in zebrafish are recruited after damage

Synaptically silent sensory hair cells in zebrafish are recruited after damage

Use of zebrafish larvae lateral line to study protection against cisplatin-induced ototoxicity: A scoping review

Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses

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