Zebrafish Tric-b is required for skeletal development and bone cells differentiation

Tonelli, Francesca; Leoni, Laura; Daponte, Valentina; Gioia, Roberta; Cotti, Silvia; Fiedler, Imke A.K.; Larianova, Daria; Willaert, Andy; Coucke, Paul; Villani, Simona; Busse, Björn; Besio, Roberta; Rossi, Antonio; Witten, P. Eckhard; Forlino, Antonella

doi:10.3389/fendo.2023.1002914

Cited by 6 publications

(2 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transmembrane Protein 38A (TMEM38A), also known as trimeric intracellular cation channel type A (TRIC-A) (33), functions as a monovalent cation channel essential for facilitating rapid intracellular calcium release. TRIC-A plays a crucial role in modulating calcium ion transport within cardiac muscle cells (34,35), and its expression is observed during the early stages of zebrafish development (34). ID4 is intricately associated with cancer, with processes such as proliferation, differentiation, migration, and invasion involving numerous cancer types (36)(37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

Exploring a novel seven-gene marker and mitochondrial gene TMEM38A for predicting cervical cancer radiotherapy sensitivity using machine learning algorithms

Wang,

Mou,

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

BackgroundRadiotherapy plays a crucial role in the management of Cervical cancer (CC), as the development of resistance by cancer cells to radiotherapeutic interventions is a significant factor contributing to treatment failure in patients. However, the specific mechanisms that contribute to this resistance remain unclear. Currently, molecular targeted therapy, including mitochondrial genes, has emerged as a new approach in treating different types of cancers, gaining significant attention as an area of research in addressing the challenge of radiotherapy resistance in cancer.MethodsThe present study employed a rigorous screening methodology within the TCGA database to identify a cohort of patients diagnosed with CC who had received radiotherapy treatment. The control group consisted of individuals who demonstrated disease stability or progression after undergoing radiotherapy. In contrast, the treatment group consisted of patients who experienced complete or partial remission following radiotherapy. Following this, we identified and examined the differentially expressed genes (DEGs) in the two cohorts. Subsequently, we conducted additional analyses to refine the set of excluded DEGs by employing the least absolute shrinkage and selection operator regression and random forest techniques. Additionally, a comprehensive analysis was conducted in order to evaluate the potential correlation between the expression of core genes and the extent of immune cell infiltration in patients diagnosed with CC. The mitochondrial-associated genes were obtained from the MITOCARTA 3.0. Finally, the verification of increased expression of the mitochondrial gene TMEM38A in individuals with CC exhibiting sensitivity to radiotherapy was conducted using reverse transcription quantitative polymerase chain reaction and immunohistochemistry assays.ResultsThis process ultimately led to the identification of 7 crucial genes, viz., GJA3, TMEM38A, ID4, CDHR1, SLC10A4, KCNG1, and HMGCS2, which were strongly associated with radiotherapy sensitivity. The enrichment analysis has unveiled a significant association between these 7 crucial genes and prominent signaling pathways, such as the p53 signaling pathway, KRAS signaling pathway, and PI3K/AKT/MTOR pathway. By utilizing these 7 core genes, an unsupervised clustering analysis was conducted on patients with CC, resulting in the categorization of patients into three distinct molecular subtypes. In addition, a predictive model for the sensitivity of CC radiotherapy was developed using a neural network approach, utilizing the expression levels of these 7 core genes. Moreover, the CellMiner database was utilized to predict drugs that are closely linked to these 7 core genes, which could potentially act as crucial agents in overcoming radiotherapy resistance in CC.ConclusionTo summarize, the genes GJA3, TMEM38A, ID4, CDHR1, SLC10A4, KCNG1, and HMGCS2 were found to be closely correlated with the sensitivity of CC to radiotherapy. Notably, TMEM38A, a mitochondrial gene, exhibited the highest degree of correlation, indicating its potential as a crucial biomarker for the modulation of radiotherapy sensitivity in CC.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring a novel seven-gene marker and mitochondrial gene TMEM38A for predicting cervical cancer radiotherapy sensitivity using machine learning algorithms

Wang,

Mou,

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…High embryo lethality may also be linked to impaired organogenesis and immunity, as evidenced by mutants in knockout genes such as LOC795232 [ 225 ], sphk1 [ 226 ], asap1a , asap1b [ 227 ], and zrsr2 [ 228 ]. The regulation of cartilage and bone formation, bone mineralization, and osteoblast differentiation involves sox9 [ 229 ], vwa1 [ 230 ], tmem38b [ 231 ], ppp2r3b [ 68 ], col11a2 [ 69 ], and wnt16 [ 232 ] genes. In common carp, the sp7a gene serves a similar function [ 24 ].…”

Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

View full text Add to dashboard Cite

Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

show abstract

Osteogenesis Imperfecta and Related Diseases: Collagen I Alterations in Skeletal and Extraskeletal Tissues

Gatto,

Pérez Franco,

Hoyer-Kuhn

et al. 2024

Biology of Extracellular Matrix

View full text Add to dashboard Cite

Zebrafish Tric-b is required for skeletal development and bone cells differentiation

Cited by 6 publications

References 66 publications

Exploring a novel seven-gene marker and mitochondrial gene TMEM38A for predicting cervical cancer radiotherapy sensitivity using machine learning algorithms

Exploring a novel seven-gene marker and mitochondrial gene TMEM38A for predicting cervical cancer radiotherapy sensitivity using machine learning algorithms

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Osteogenesis Imperfecta and Related Diseases: Collagen I Alterations in Skeletal and Extraskeletal Tissues

Contact Info

Product

Resources

About