Zebrafish Vascular Development: General and Tissue-Specific Regulation

Nakajima, Hiroyuki; Chiba, Ayano; Fukumoto, Moe; Morooka, Nanami; Mochizuki, Naoki

doi:10.12997/jla.2021.10.2.145

Cited by 12 publications

(10 citation statements)

References 87 publications

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“…In recent decades, numerous disease models have been established using zebrafish larvae to study developmental processes, such as hematology [ 194 , 195 ], and pathogenic processes in cancer [ 196 ] and infectious diseases [ 197 ]. Zebrafish models have contributed to uncovering pathogenic mechanisms and to the discovery and efficacy screening of innovative drugs [ 198 , 199 ].…”

Section: Zebrafish As a Model To Investigate Tlr2 As A Therapeutic Ta...mentioning

confidence: 99%

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

Spaink

2022

Biology

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Innate immunity is considered the first line of defense against microbial invasion, and its dysregulation can increase the susceptibility of hosts to infections by invading pathogens. Host cells rely on pattern recognition receptors (PRRs) to recognize invading pathogens and initiate protective innate immune responses. Toll-like receptor 2 (TLR2) is believed to be among the most important Toll-like receptors for defense against mycobacterial infection. TLR2 has been reported to have very broad functions in infectious diseases and also in other diseases, such as chronic and acute inflammatory diseases, cancers, and even metabolic disorders. However, TLR2 has an unclear dual role in both the activation and suppression of innate immune responses. Moreover, in some studies, the function of TLR2 was shown to be controversial, and therefore its role in several diseases is still inconclusive. Therefore, although TLR2 has been shown to have an important function in innate immunity, its usefulness as a therapeutic target in clinical application is still uncertain. In this literature review, we summarize the knowledge of the functions of TLR2 in host–mycobacterial interactions, discuss controversial results, and suggest possibilities for future research.

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Section: Zebrafish As a Model To Investigate Tlr2 As A Therapeutic Ta...mentioning

confidence: 99%

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

Spaink

2022

Biology

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“…Due to constant technical progress, zebrafish also became a powerful tool for in vivo imaging of blood and lymphatic development [118,121]. The generation of transgenic zebrafish lines that express fluorescent labeled vasculature enabled high-resolution real-time imaging of vessels [122].…”

Section: Zebrafishmentioning

confidence: 99%

In Vitro, In Vivo, and In Silico Models of Lymphangiogenesis in Solid Malignancies

Bekisz

Baudin

Buntinx

et al. 2022

Cancers

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Lymphangiogenesis (LA) is the formation of new lymphatic vessels by lymphatic endothelial cells (LECs) sprouting from pre-existing lymphatic vessels. It is increasingly recognized as being involved in many diseases, such as in cancer and secondary lymphedema, which most often results from cancer treatments. For some cancers, excessive LA is associated with cancer progression and metastatic dissemination to the lymph nodes (LNs) through lymphatic vessels. The study of LA through in vitro, in vivo, and, more recently, in silico models is of paramount importance in providing novel insights and identifying the key molecular actors in the biological dysregulation of this process under pathological conditions. In this review, the different biological (in vitro and in vivo) models of LA, especially in a cancer context, are explained and discussed, highlighting their principal modeled features as well as their advantages and drawbacks. Imaging techniques of the lymphatics, complementary or even essential to in vivo models, are also clarified and allow the establishment of the link with computational approaches. In silico models are introduced, theoretically described, and illustrated with examples specific to the lymphatic system and the LA. Together, these models constitute a toolbox allowing the LA research to be brought to the next level.

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“…Good CCC can reduce adverse cardiovascular events and infarct size when coronary arteries are occluded [ 1 ]. The involvement of growth factors, cytokines, and shear stress in collateral circulation development has been previously reported [ 1 , 2 ]. However, the factors and predictors associated with collateral circulation are incompletely understood, with limited evidence on epigenetic impact.…”

Section: Introductionmentioning

confidence: 99%

Differentially hypomethylated cell-free DNA and coronary collateral circulation

Ahn

Heo²,

Ahn

et al. 2022

Clin Epigenet

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Background The factors affecting cardioprotective collateral circulation are still incompletely understood. Recently, characteristics, such as CpG methylation of cell-free DNA (cfDNA), have been reported as markers with clinical utility. The aim of this study was to evaluate whether cfDNA methylation patterns are associated with the grade of coronary collateral circulation (CCC). Result In this case–control study, clinical and angiographic data were obtained from 143 patients (mean age, 58 years, male 71%) with chronic total coronary occlusion. Enzymatic methyl-sequencing (EM-seq) libraries were prepared using the cfDNA extracted from the plasma. Data were processed to obtain the average methylation fraction (AMF) tables of genomic regions from which blacklisted regions were removed. Unsupervised analysis of the obtained AMF values showed that some of the changes in methylation were due to CCC. Through random forest preparation process, 256 differentially methylated region (DMR) candidates showing strong association with CCC were selected. A random forest classifier was then constructed, and the area under the curve of the receiver operating characteristic curve indicated an appropriate predictive function for CCC. Finally, 20 DMRs were identified to have significantly different AMF values between the good and poor CCC groups. Particularly, the good CCC group exhibited hypomethylated DMRs. Pathway analysis revealed five pathways, including TGF-beta signaling, to be associated with good CCC. Conclusion These data have demonstrated that differential hypomethylation was identified in dozens of cfDNA regions in patients with good CCC. Our results support the clinical utility of noninvasively obtained epigenetic signatures for predicting collateral circulation in patients with vascular diseases.

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Zebrafish Vascular Development: General and Tissue-Specific Regulation

Cited by 12 publications

References 87 publications

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

The Role of TLR2 in Infectious Diseases Caused by Mycobacteria: From Cell Biology to Therapeutic Target

In Vitro, In Vivo, and In Silico Models of Lymphangiogenesis in Solid Malignancies

Differentially hypomethylated cell-free DNA and coronary collateral circulation

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