Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth

Jackson, Henry Wells; Prakash, Divakar; Litaker, Mark S.; Ferreira, Tracie; Jezewski, Peter A.

doi:10.4236/ajmb.2015.53006

Cited by 6 publications

(4 citation statements)

References 73 publications

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“… 91 )) of the WNT-signaling pathway. Defects or expression disruptions of wnt9b , tpbg , sfrp5 and pou2f1 genes lead to deficiency in the WNT signal pathway, and bilateral craniofacial asymmetry and skull malformation in vertebrates 92 , 93 , including zebrafish 94 . Our analyses also revealed substantial changes in physicochemical properties and three-dimensional structure of these WNT components in RFP (for example, T212P and P428S cause polarity changes of amino acids in pou2f1; T169K caused charge changes in tpbg; K236R caused protein structure changes in sfrp5) (Supplementary Figs.…”

Section: Resultsmentioning

confidence: 99%

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

et al. 2021

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The evolutionary and genetic origins of the specialized body plan of flatfish are largely unclear. We analyzed the genomes of 11 flatfish species representing 9 of the 14 Pleuronectiforme families and conclude that Pleuronectoidei and Psettodoidei do not form a monophyletic group, suggesting independent origins from different percoid ancestors. Genomic and transcriptomic data indicate that genes related to WNT and retinoic acid pathways, hampered musculature and reduced lipids might have functioned in the evolution of the specialized body plan of Pleuronectoidei. Evolution of Psettodoidei involved similar but not identical genes. Our work provides valuable resources and insights for understanding the genetic origins of the unusual body plan of flatfishes.

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Section: Resultsmentioning

confidence: 99%

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

et al. 2021

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“…The third gene, wnt9b , is a member of the WNT gene family encoding a secreted signaling protein required for dorso-ventral patterning of oral jaw and outgrowth of upper jaw in zebrafish [42, 56]. Expectedly, we found higher oral jaw expression of wnt9b in all species except the herbivorous species from LT, also, more similar expression patterns for wnt9b were found in the jaws of species from LM and LV.…”

Section: Discussionmentioning

confidence: 51%

Divergence in larval jaw gene expression reflects differential trophic adaptation in haplochromine cichlids prior to foraging

et al. 2019

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Background Understanding how variation in gene expression contributes to morphological diversity is a major goal in evolutionary biology. Cichlid fishes from the East African Great lakes exhibit striking diversity in trophic adaptations predicated on the functional modularity of their two sets of jaws (oral and pharyngeal). However, the transcriptional basis of this modularity is not so well understood, as no studies thus far have directly compared the expression of genes in the oral and pharyngeal jaws. Nor is it well understood how gene expression may have contributed to the parallel evolution of trophic morphologies across the replicate cichlid adaptive radiations in Lake Tanganyika, Malawi and Victoria. Results We set out to investigate the role of gene expression divergence in cichlid fishes from these three lakes adapted to herbivorous and carnivorous trophic niches. We focused on the development stage prior to the onset of exogenous feeding that is critical for understanding patterns of gene expression after oral and pharyngeal jaw skeletogenesis, anticipating environmental cues. This framework permitted us for the first time to test for signatures of gene expression underlying jaw modularity in convergent eco-morphologies across three independent adaptive radiations. We validated a set of reference genes, with stable expression between the two jaw types and across species, which can be important for future studies of gene expression in cichlid jaws. Next we found evidence of modular and non-modular gene expression between the two jaws, across different trophic niches and lakes. For instance, prdm1a , a skeletogenic gene with modular anterior-posterior expression, displayed higher pharyngeal jaw expression and modular expression pattern only in carnivorous species. Furthermore, we found the expression of genes in cichlids jaws from the youngest Lake Victoria to exhibit low modularity compared to the older lakes. Conclusion Overall, our results provide cross-species transcriptional comparisons of modularly-regulated skeletogenic genes in the two jaw types, implicating expression differences which might contribute to the formation of divergent trophic morphologies at the stage of larval independence prior to foraging. Electronic supplementary material The online version of this article (10.1186/s12862-019-1483-3) contains supplementary material, which is available to authorized users.

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“…The following MOs were injected into the yolk at the one-cell stage in 1 nl total volume: wnt9a (5′-AAGAATTGTCCTGCCTACCCGAAGT-3′) (1 ng/embryo) ( Paolini et al, 2021 ), wnt9b (5′-ACCTGTAAGCCTAACGAAAACACAA-3′) (1 ng/embryo) ( Jackson et al, 2015 ), klf2a (5′-CTCGCCTATGAAAGAAGAGAGGATT-3′) (1 ng/embryo) ( Nicoli et al, 2010 ), klf2b (5′-AAAGGCAAGGTAAAGCCATGTCCAC-3′) (5 ng/embryo) ( Renz et al, 2015 ), cloche/npas4l (5′-GAGTCTCCGCAGCTCATCTCACA-3′) (1 ng/embryo) ( Reischauer et al, 2016 ). Control embryos (WT) were always injected with 1 nl of water.…”

Section: Methodsmentioning

confidence: 99%

Wnt9 directs zebrafish heart tube assembly via a combination of canonical and non-canonical pathway signaling

Paolini,

Sharipova,

Lange

et al. 2023

Development

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During zebrafish heart formation, cardiac progenitor cells converge at the embryonic midline where they form the cardiac cone. Subsequently, this structure transforms into a heart tube. Only little is known about the molecular mechanisms that control these morphogenetic processes. Here, we use light sheet microscopy and combine genetic, molecular biological and pharmacological tools to show that the paralogous genes wnt9a/b are required for the assembly of the nascent heart tube. In wnt9a/b double mutants, cardiomyocyte progenitor cells are delayed in their convergence towards the embryonic midline, the formation of the heart cone is impaired and the transformation into an elongated heart tube fails. The same cardiac phenotype occurs when both canonical and non-canonical Wnt signaling pathways are simultaneously blocked by pharmacological inhibition. This demonstrates that Wnt9a/b and canonical and non-canonical Wnt signaling regulate the migration of cardiomyocyte progenitor cells and control the formation of the cardiac tube. This can be partly attributed to their regulation of the timing of cardiac progenitor cell differentiation. Our study demonstrates how these morphogens activate a combination of downstream pathways to direct cardiac morphogenesis.

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Zebrafish Wnt9b Patterns the First Pharyngeal Arch into D-I-V Domains and Promotes Anterior-Medial Outgrowth

Cited by 6 publications

References 73 publications

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

Large-scale sequencing of flatfish genomes provides insights into the polyphyletic origin of their specialized body plan

Divergence in larval jaw gene expression reflects differential trophic adaptation in haplochromine cichlids prior to foraging

Wnt9 directs zebrafish heart tube assembly via a combination of canonical and non-canonical pathway signaling

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