Zeta potential vs apoptotic marker: which is more suitable for ICSI sperm selection?

Zahedi, Atena; Tavalaee, Marziyeh; Deemeh, Mohammad Reza; Azadi, Leila; Fazilati, Mohammad; Nasr-Esfahani, Mohammad Hossein

doi:10.1007/s10815-013-0022-1

Cited by 26 publications

(16 citation statements)

References 29 publications

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“…The identification and elimination of “apoptotic” spermatozoa, those showing phosphatidylserine (PS) transposition, is a relatively common procedure in human fertility clinics. It is believed that the elimination of these apoptotic spermatozoa using magnetic beads increases fertility (Zahedi et al., ). The membrane of the mammalian spermatozoa can experience damage that is not detected using classical dye exclusion assays.…”

Section: The Membrane: Increased Permeability Phospholipid Transposimentioning

confidence: 99%

Flow cytometry analysis of spermatozoa: Is it time for flow spermetry?

Peña

Rodriguez

Gil

et al. 2018

Reprod Domestic Animals

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Contents Flow cytometry is increasingly used in research and also in clinical andrology. Recent developments in instrumentation, availability of probes and bioinformatics expand the possibilities of flow cytometry well beyond the classical two parametric analyses in use. In this paper, an overview of recent developments in flow cytometry will be presented under the perspective of the authors; aspects such a multicolor assays and computational cytometry will be discussed as well.

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Section: The Membrane: Increased Permeability Phospholipid Transposimentioning

confidence: 99%

Flow cytometry analysis of spermatozoa: Is it time for flow spermetry?

Peña

Rodriguez

Gil

et al. 2018

Reprod Domestic Animals

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show abstract

“…Externalization of PS from inner to outer layer of plasma membrane can be assessed by Annexin V and is considered as one of the early signs of apoptosis before capacitation. [43] However, this process is a nor- Steps of acrosome reaction mal phenomenon observed during capacitation. This dual functioning role of PS in human spermatozoa makes interpretation and test design very difficult.…”

Section: Hyaluronan Binding Assay (Hba)mentioning

confidence: 99%

“…It has been emphasized that the MACS assay has the potential to isolate spermatozoa with normal acrosomes and protamine content and to have a higher significance in severe teratozoospermic patients. [46] Zeta sperm selection A mature sperm possess a surface electric charge ranging from -16 to -20 mV, termed as the zeta potential which decreases with capacitation. [47] Normally differentiated sperms are charged electronegatively ( Figure 7).…”

Section: Hyaluronan Binding Assay (Hba)mentioning

confidence: 99%

Sperm function tests in clinical practice

Kızılay¹,

Altay²

2017

Turkish Journal of Urology

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Conventional semen analysis solely is not completely adequate to predict pregnancy outcomes. Therefore, advanced sperm function tests have been developed and introduced to clinical practice. These tests use different methods and techniques to evaluate different stages of fertilization steps. In this review, we reported some commonly used sperm function tests: sperm penetration assay, sperm-zona pellucida binding test (hemizona assay), acrosomal reaction test, hyaluronan binding test, hypo-osmotic swelling test, magneticactivated cell sorting and zeta sperm selection. We discussed the literature concerning these tests, the utilization techniques and also purpose and mechanism of each test. We emphasized the importance of sperm function tests in predicting in vitro fertilization and pregnancy outcomes and in the management of infertile couples and also the limitations of these tests. Along with improvements in molecular biology techniques, we believe that more applicative and beneficial tests will be developed in the near future.

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“…Slides were observed under a microscope (Zeiss, Oberkochen, Germany) equipped with a 100-magnification lens. [54][55][56][57][58][59][60] Spermatozoa with fragmented DNA had browncolored nuclei, whereas the other cells were blue-gray (counter coloration with Harris's haematoxylin) [7,[61][62][63]. On each slide, approximately 500 cells were counted, and the DNA fragmentation index (DFI) was calculated ( Figure 2).…”

Section: Measurement Of Dna Fragmentationmentioning

confidence: 99%

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Ben Ali

2017

Andrology

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We aimed to compare the effect of TGFB1 and glutathione on sperm necrosis in three groups of patients with increased necrospermia. The study included 120 men aged 37.6 ± 4.6 years consulting for sterility of the couple. Patients were subdivided into three groups according to the percentage of necrotic spermatozoa: necrospermia<30%; N=40), moderate necrozoospermia (50-80%, n=45) and severe necrozoospermia (>80%) For each patient, the sperm parameters were evaluated according to WHO standards, TGFB1 and glutathione Oxidized and reduced was carried out by the ELISA technique and by spectrophotometry respectively. We found a significant increase in the levels of TGFβ1 and DFI in moderate and severe necrospermia groups and positive correlations between these two factors and sperm parameters (numeration, mobility and morphology). In addition, a significant decrease in seminal GHSt was observed in the necrozoospermic groups compared to the control group. A strong correlation was observed between the degree of necrozoospermia and the fragmentation of sperm DNA (r=0.878, p=0.001). In addition, a significant positive correlation between TGFβ1 and DFI in the two groups of patients with moderate necrospermia (r=0.43, p<0.05) and severe necrospermia (r=0.52, p<0.05). This confirms that seminal TGFβ1 is a factor in the fragmentation of spermatozoa DNA. These results suggest that decreased glutathione and increased seminal TGFβ1 are important risk factors that can lead to a succession of morphological and functional alterations of spermatozoa resulting in necrozoospermia. Their perturbation in seminal plasma can lead to mediocre results of medically assisted procreation.

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Zeta potential vs apoptotic marker: which is more suitable for ICSI sperm selection?

Cited by 26 publications

References 29 publications

Flow cytometry analysis of spermatozoa: Is it time for flow spermetry?

Flow cytometry analysis of spermatozoa: Is it time for flow spermetry?

Sperm function tests in clinical practice

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

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