Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus

Onyango, Maria G; Lange, Rachel E.; Bialosuknia, Sean M.; Payne, Anne F.; Mathias, Nicholas; Kuo, Lili; Vigneron, Aurélien; Nag, Dilip K.; Kramer, Laura D.; Ciota, Alexander T.

doi:10.1186/s13071-021-05069-7

Cited by 25 publications

(36 citation statements)

References 89 publications

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“…aegypti. Also, the number of bacterial ASVs shared by all groups for females (19) and males (24) was lower than the total number of bacterial ASVs for females (42) and males (71), suggesting a small core bacterial community that was not altered by the presence of MPs in the gut.…”

Section: Discussionmentioning

confidence: 95%

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Microplastic ingestion perturbs the microbiome of Aedes albopictus and Aedes aegypti

Edwards

McConnel

Ramos

et al. 2023

Preprint

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Microplastics (MPs) are common environmental pollutants; however, little is known about their effects after ingestion by insects. Here we exposed Aedes aegypti and Aedes albopictus mosquito larvae to 1 µm polystyrene MPs and examined the impacts MP ingestion on adult emergence rates, gut damage, and fungal and bacterial microbiota. Results show that ingested MPs accumulate in the larval guts, resulting in gut damage. However, little impact on adult emergence rates was observed. MPs are also found in adult guts post-emergence from the pupal stage, and adults expel MPs in their frass after obtaining sugar meals. Moreover, MPs effects on insect microbiomes need to be better defined. To address this knowledge gap, we investigated the relationship between MP ingestion and the gut microbial communities in Ae. albopictus and Ae. aegypti. Notably, the microbiota composition was altered by the ingestion of increasing concentrations of MPs. Amplicon sequence variants (ASV) that contributed to differences in the bacterial and fungal microbiota composition between MP treatments were from the genera Elizabethkingia and Aspergillus, respectively. Furthermore, a decrease in the alpha diversity of the fungal and bacterial microbiota was observed in treatments where larvae ingested MPs. These results highlight the potential for the bacterial and fungal constituents in the mosquito microbiome to respond differently to the ingestion of MPs as larvae. Based on our findings and the effects of MP ingestion on the mosquito host micro- and mycobiome, MP pollution could impact the vector competence of important mosquito-transmitted viruses and parasites that cause human and animal diseases.

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Section: Discussionmentioning

confidence: 95%

“…Elizabethkingia anophelis has been demonstrated to affect Plasmodium development in Anopheles mosquitoes and Zika virus infection rates in Ae. albopictus [42,43] . Elizabethkingia density changes as the result of MP ingestion suggests the potential for MPs to indirectly affect arbovirus transmission by Ae.…”

Section: Discussionmentioning

confidence: 99%

Microplastic ingestion perturbs the microbiome of Aedes albopictus and Aedes aegypti

Edwards

McConnel

Ramos

et al. 2023

Preprint

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“…aegypti with experimental ZIKV infection have found associated bacterial taxa such as the families Rhodobacteraceae and Desulfuromonadaceae and the genera Cellulosimicrobium, Elizabethkingia, Enterobacter, Pseudomonas, and Staphylococcus ; in Ae. albopictus , Elizabethkingia anophelis was associated with ZIKV infection [ 25 , 84 , 96 ]. Additionally, E. anophelis and Lysinibacillus reduced ZIKV infection, affecting vector competence [ 84 , 96 ].…”

Section: Discussionmentioning

confidence: 99%

“…albopictus , Elizabethkingia anophelis was associated with ZIKV infection [ 25 , 84 , 96 ]. Additionally, E. anophelis and Lysinibacillus reduced ZIKV infection, affecting vector competence [ 84 , 96 ].…”

Section: Discussionmentioning

confidence: 99%

Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations

Arévalo-Cortés

Damania

Granada

et al. 2022

Viruses

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Introduction: Aedes aegypti is the vector of several arboviruses such as dengue, Zika, and chikungunya. In 2015–16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome. This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector. The conditions of cocirculation of these three arboviruses, failure in vector control due to insecticide resistance, limitations in dengue management during the COVID-19 pandemic, and lack of effective treatment or vaccines make it necessary to identify changes in mosquito midgut bacterial composition and predict its functions through the infection. Its study is fundamental because it generates knowledge for surveillance of transmission and the risk of outbreaks of these diseases at the local level. Methods: Midgut bacterial compositions of females of Colombian Ae. aegypti populations were analyzed using DADA2 Pipeline, and their functions were predicted with PICRUSt2 analysis. These analyses were done under the condition of natural ZIKV infection and resistance to lambda–cyhalothrin, alone and in combination. One-step RT-PCR determined the percentage of ZIKV-infected females. We also measured the susceptibility to the pyrethroid lambda–cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene. Results: We found high ZIKV infection rates in Ae. aegypti females from Colombian rural municipalities with deficient water supply, such as Honda with 63.6%. In the face of natural infection with an arbovirus such as Zika, the diversity between an infective and non-infective form was significantly different. Bacteria associated with a state of infection with ZIKV and lambda–cyhalothrin resistance were detected, such as the genus Bacteroides, which was related to functions of pathogenicity, antimicrobial resistance, and bioremediation of insecticides. We hypothesize that it is a vehicle for virus entry, as it is in human intestinal infections. On the other hand, Bello, the only mosquito population classified as susceptible to lambda–cyhalothrin, was associated with bacteria related to mucin degradation functions in the intestine, belonging to the Lachnospiraceae family, with the genus Dorea being increased in ZIKV-infected females. The Serratia genus presented significantly decreased functions related to phenazine production, potentially associated with infection control, and control mechanism functions for host defense and quorum sensing. Additionally, Pseudomonas was the genus principally associated with functions of the degradation of insecticides related to tryptophan metabolism, ABC transporters with a two-component system, efflux pumps, and alginate synthesis. Conclusions: Microbiota composition may be modulated by ZIKV infection and insecticide resistance in Ae. aegypti Colombian populations. The condition of resistance to lambda–cyhalothrin could be inducing a phenome of dysbiosis in field Ae. aegypti affecting the transmission of arboviruses.

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“…inhibits viral and parasite infection in A. aegypti cells and A. gambiae mosquitoes through protease or depsipeptide synthesis [61,62]. It was already shown that Elizabethkingia anophelis , a bacterium of the phylum Bacteroidetes, artificially fed to A. albopictus reduces ZKV infection rates [63]. On the other hand, Serratia odorifera gut colonization increases A. aegypti susceptibility to Dengue virus infection [64], Penicillium chrysogenum fungus facilitates A. gambiae infection with Plasmodium via up-regulation of an arginine digestion enzyme preventing the production of nitric oxide, a known microbicidal radical [65].…”

Section: Discussionmentioning

confidence: 99%

An Aedes aegypti seryl-tRNA synthetase paralog controls bacteroidetes growth in the midgut

Silveira

Talyuli

Walter-Nuno

et al. 2022

Preprint

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Insect gut microbiota plays important roles in host physiology, such as nutrition, digestion, development, fertility, and immunity. We have found that in the intestine of Aedes aegypti, SLIMP (seryl-tRNA synthetase like insect mitochondrial protein) knockdown followed by a blood meal promotes dysbiosis, characterized by the overgrowth of a specific bacterial phylum, Bacteroidetes. In turn, the latter decreased both infection rates and Zika virus prevalence in the mosquitoes. Previous work in Drosophila melanogaster showed that SLIMP is involved in protein synthesis and mitochondrial respiration in a network directly coupled to mtDNA levels. There are no other reports on this enzyme and its function in other insect species. Our work expands the knowledge of the role of these SerRS paralogs. We show that A. aegypti SLIMP (AaeSLIMP) clusters with SLIMPs of the Nematocera sub-order, which have lost both the tRNA binding domain and active site residues, rendering them unable to activate amino acids and aminoacylate tRNAs. Knockdown of AaeSLIMP did not significantly influence the mosquitoes’ survival, oviposition, or eclosion. It also neither affected midgut cell respiration nor mitochondrial ROS production. However, it caused dysbiosis, which led to the activation of Dual oxidase and resulted in increased midgut ROS levels. Our data indicate that the intestinal microbiota can be controlled in a blood-feeding vector by a novel, unprecedent mechanism, impacting also mosquito vectorial competence towards zika virus and possibly other pathogens as well.

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Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus

Cited by 25 publications

References 89 publications

Microplastic ingestion perturbs the microbiome of Aedes albopictus and Aedes aegypti

Microplastic ingestion perturbs the microbiome of Aedes albopictus and Aedes aegypti

Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Aedes aegypti Populations

An Aedes aegypti seryl-tRNA synthetase paralog controls bacteroidetes growth in the midgut

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