Zika virus as a causative agent for primary microencephaly: the evidence so far

Tang, Bor Luen

doi:10.1007/s00203-016-1268-7

Cited by 26 publications

(18 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a result, our work emphasizes the need to focus research efforts on unraveling the true source of these microcephaly cases, keeping in mind that it may involve a complex combination of causes. As the ZIKV infection is now becoming more and more strongly correlated with the emergence of these microcephaly phenotypes42525354, we recommend that the scientific community continue to pursue research along this path.…”

Section: Discussionmentioning

confidence: 99%

The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos

Dzieciolowska

Larroque

Kranjec

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Although the zika virus (ZIKV) has now been strongly correlated with emerging cases of microcephaly in the Americas, suspicions have been raised regarding the use of pyriproxyfen, a larvicide that prevents mosquito development, in drinking water. The effects of this compound on neurodevelopment have not yet been addressed specifically in vertebrates. As a result, we aimed at addressing the effects, if any, of pyriproxyfen on neurodevelopment in the zebrafish embryo as a vertebrate model. Using zebrafish transgenic lines expressing GFP in different cell populations (elavl3 in newborn neurons, gfap and nestin in neural stem cells), we focused on the analysis of whole embryonic brain volume after confocal 3D-reconstruction and the quantification of purified neural stem cells during early neurodevelopment by FACS-cell sorting from whole in vivo embryos. Interestingly, though lethal at very high doses, pyriproxyfen did not cause brain malformation nor any significant changes in the number of observed stem cells in the developing central nervous system. Our data indicate that pyriproxyfen does not affect central nervous system development in zebrafish, suggesting that this larvicide on its own, may not be correlated with the increase in microcephaly cases reported recently.

show abstract

Section: Discussionmentioning

confidence: 99%

The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos

Dzieciolowska

Larroque

Kranjec

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…We examined three different HEK293-derivatives, HEK293-FIT, HEK293-T3Y and 293T cells, and found they supported varying degrees of ZIKV replication (see below). A notable feature of ZIKV infection in humans is its neurotropism [46][47][48]. We thus examined two human cell lines of neural origin, i.e., the neuroblastoma line SK-N-SH and SVGA, a fetal glial cell line, and found both to be permissive for ZIKV infection (S3 Fig).…”

Section: Broad Tropism Of Zikv In Cell Culturementioning

confidence: 99%

The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function

Yang

Wei

et al. 2019

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Infection by Zika virus (ZIKV) is linked to microcephaly and other neurological disorders, posing a significant health threat. Innate immunity is the first line of defense against invading pathogens, but relatively little is understood regarding host intrinsic mechanisms that guard against ZIKV. Here, we show that host tripartite motif-containing protein 56 (TRIM56) poses a barrier to ZIKV infection in cells of neural, epithelial and fibroblast origins. Overexpression of TRIM56, but not an E3 ligase-dead mutant or one lacking a short C-terminal portion, inhibited ZIKV RNA replication. Conversely, depletion of TRIM56 increased viral RNA levels. Although the C-terminal region of TRIM56 bears sequence homology to NHL repeat of TRIM-NHL proteins that regulate miRNA activity, knockout of Dicer, which abolishes production of miRNAs, had no demonstrable effect on ZIKV restriction imposed by TRIM56. Rather, we found that TRIM56 is an RNA-binding protein that associates with ZIKV RNA in infected cells. Moreover, a recombinant TRIM56 fragment comprising the C-terminal 392 residues captured ZIKV RNA in cell-free reactions, indicative of direct interaction. Remarkably, deletion of a short C-terminal tail portion abrogated the TRIM56-ZIKV RNA interaction, concomitant with a loss in antiviral activity. Altogether, our study reveals TRIM56 is an RNA binding protein that acts as a ZIKV restriction factor and provides new insights into the antiviral mechanism by which this E3 ligase tackles flavivirus infections.

show abstract

“…In pregnant women, the disease can cause fetal brain damage and microcephaly, whereas in adults it causes Guillain-Barré syndrome. Zika virus was also found in semen, and sexual transmission has been reported 34 . The attack rate is estimated at 14.6/1,000 inhabitants during epidemics; however, the exact risk for travelers remains unknown 35 .…”

Section: Other Arbovirus: Dengue Chikungunya and Zikamentioning

confidence: 99%

Preventing diseases in round-the-world travelers: a contemporary challenge for travel medicine advice

Rodrigues

Moreira

2018

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Providing advice for travelers embarking on long-term trips poses a challenge in travel medicine. A long duration of risk exposure is associated with underuse of protective measures and poor adherence to chemoprophylaxis, increasing the chances of acquiring infections. Recently, in our clinic, we observed an increase in the number of travelers undertaking round-the-world trips. These individuals are typically aged around 32 years and quit their jobs to embark on one-to-two-year journeys. Their destinations include countries in two or more continents, invariably Southeast Asia and Indonesia, and mostly involve land travel and visiting rural areas. Such trips involve flexible plans, increasing the challenge, especially with regard to malaria prophylaxis. Advising round-the-world travelers is time-consuming because of the amount of information that must be provided to the traveler. Advisors must develop strategies to commit the traveler to his/her own health, and verify their learnings on disease-prevention measures. Contacting the advisor after the appointment or during the trip can be helpful to clarify unclear instructions or diagnosis made and prescriptions given abroad. Infectious diseases are among the most frequent problems affecting travelers, many of which are preventable by vaccines, medicines, and precautionary measures. The dissemination of counterfeit medicines, particularly antibiotics and antimalarial medicines, emphasizes the need for travelers to carry medicines that they may possibly need on their trip. Additional advice on altitude, scuba diving, and other possible risks may also be given. Considering the difficulties in advising this group, we present a review of the main recommendations on advising these travelers.

show abstract

Zika virus as a causative agent for primary microencephaly: the evidence so far

Cited by 26 publications

References 68 publications

The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos

The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos

The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function

Preventing diseases in round-the-world travelers: a contemporary challenge for travel medicine advice

Contact Info

Product

Resources

About