Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil

Ayres, Constância Flávia Junqueira; Guedes, Duschinka Ribeiro Duarte; Paiva, Marcelo Henrique Santos; Morais-Sobral, Mariana C.; Krokovsky, Larissa; Machado, Laís Ceschini; Melo-Santos, Maria Alice Varjal de; Crespo, Mônica Maria; Oliveira, Cláudia Maria Fontes de; Ribeiro, Ricardo Silva; Cardoso, Orlei Amaral; Menezes, Ana Lúcia Barbosa; Laperrière-Jr, Roberto Costa; Luna, Carlos Feitosa; Oliveira, André Luiz Sá de; Leal, Walter Soares; Wallau, Gabriel Luz

doi:10.1186/s13071-019-3461-4

Cited by 24 publications

(16 citation statements)

References 58 publications

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“…aegypti, (28) although Culex quinquefasciatus females have been collected naturally infected with ZIKV. (29) Besides that, the presence of Ae. albopictus in Brazil was first recorded in 1986 in Rio de Janeiro, (30) while in Recife it was detected only after 1996, within PNCD larval surveillance.…”

Section: Discussionmentioning

confidence: 99%

Infestation of an endemic arbovirus area by sympatric populations of Aedes aegypti and Aedes albopictus in Brazil

Barbosa

Melo-Santos

Silveira

et al. 2020

Mem. Inst. Oswaldo Cruz

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BACKGROUND Aedes aegypti and Aedes albopictus are the most important arbovirus vectors in the world. OBJECTIVES This study aimed to investigate and compare the infestation pattern of these species in a neighbourhood of Recife, Brazil, endemic for arboviruses in 2005 (T1) and 2013 (T2). METHODS Infestation, distribution and relative abundance of these sympatric species were recorded by egg collection using a network of 59 sentinel ovitraps (s-ovt) at fixed sampling stations for 12 months in T1 and T2. FINDINGS A permanent occupation pattern was detected which was characterised by the presence of egg-laying females of one or both species with a high ovitrap positivity index (94.3 to 100%) throughout both years analysed. In terms of abundance, the total of eggs collected was lower (p < 0.005) in T2 (146,153) than in T1 (281,103), although ovitraps still displayed a high index of positivity. The spatial distribution showed the presence of both species in 65.1% of the 148 s-ovt assessed, while a smaller number of traps exclusively contained Ae. aegypti (22%) or Ae. albopictus (13.2%) eggs. MAIN CONCLUSIONS Our comparative analysis demonstrated the robustness of the spatial occupation and permanence of Ae. aegypti and Ae. albopictus populations in this endemic urban area.

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Section: Discussionmentioning

confidence: 99%

Infestation of an endemic arbovirus area by sympatric populations of Aedes aegypti and Aedes albopictus in Brazil

Barbosa

Melo-Santos

Silveira

et al. 2020

Mem. Inst. Oswaldo Cruz

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“…aegypti cooccurs with other domestic and peridomestic mosquitoes such as Culex quinquefasciatus and Aedes albopictus that frequently bite humans. Natural ZIKV infections have been reported in these species, and thus, they came under suspicion as alternative vectors [ 24 , 25 , 26 , 27 ]. Concerning Ae.…”

Section: Introductionmentioning

confidence: 99%

Vector Competence of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus from Brazil and New Caledonia for Three Zika Virus Lineages

et al. 2020

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Zika virus (ZIKV) has caused severe epidemics in South America beginning in 2015, following its spread through the Pacific. We comparatively assessed the vector competence of ten populations of Aedes aegypti and Ae. albopictus from Brazil and two of Ae. aegypti and one of Culex quinquefasciatus from New Caledonia to transmit three ZIKV isolates belonging to African, Asian and American lineages. Recently colonized mosquitoes from eight distinct sites from both countries were orally challenged with the same viral load (107 TCID50/mL) and examined after 7, 14 and 21 days. Cx. quinquefasciatus was refractory to infection with all virus strains. In contrast, although competence varied with geographical origin, Brazilian and New Caledonian Ae. aegypti could transmit the three ZIKV lineages, with a strong advantage for the African lineage (the only one reaching saliva one-week after challenge). Brazilian Ae. albopictus populations were less competent than Ae. aegypti populations. Ae. albopictus generally exhibited almost no transmission for Asian and American lineages, but was efficient in transmitting the African ZIKV. Viral surveillance and mosquito control measures must be strengthened to avoid the spread of new ZIKV lineages and minimize the transmission of viruses currently circulating.

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“…In another 16 studies, ZIKV infection was confirmed but without infected salivary glands or transmission (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42). In a study conducted in Kédougou, Senegal (30), the ZIKV was identified in a single female Culex perfuscus (Edwards) mosquito.…”

Section: Study Selectionmentioning

confidence: 98%

Vector competence of Culex mosquitoes (Diptera: Culicidae) in Zika virus transmission: an integrative review

Rosa

Regis

Santos

2020

Revista Panamericana De Salud Pública

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Objective. To identify studies on the competence of Culex mosquitoes as vectors for the transmission of Zika virus (ZIKV) around the globe. Methods. We performed an integrative review to identify relevant articles on specific experiments to determine whether Culex mosquitoes are vectors for ZIKV. The sources we used for our research were the Brazilian Periódicos CAPES electronic portal (MEDLINE/PubMed, ScienceDirect Journals, Nature Publishing Group, SciELO, Springer Link, and 250 other databases) and gray literature. Results. We identified 344 studies, of which 36 were considered for this review. In 8 studies, infection in salivary glands of Culex quinquefasciatus, Culex restuans, Culex tarsalis, and Culex coronator was detected. Cx. quinquefasciatus was the most studied among those confirmed as potential ZIKV vectors, and only strains of Asian lineages (THA/2014/SV0127-14; SZ01 (2016)) and American lineages (BRPE243 (2015); PRVABC59 (2015)) can infect the salivary glands of Culex mosquitoes. The tested African strains (MR766 and DAK AR 41525) were unable to infect salivary glands. Conclusions. There is still a lack of compelling evidence that indicates Culex spp. are a competent ZIKV vector, but they should remain a target for further monitoring studies, especially regarding ZIKV transmission to other species. Furthermore, studies should not be limited to studying whether their salivary glands are infected.

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Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil

Cited by 24 publications

References 58 publications

Infestation of an endemic arbovirus area by sympatric populations of Aedes aegypti and Aedes albopictus in Brazil

Infestation of an endemic arbovirus area by sympatric populations of Aedes aegypti and Aedes albopictus in Brazil

Vector Competence of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus from Brazil and New Caledonia for Three Zika Virus Lineages

Vector competence of Culex mosquitoes (Diptera: Culicidae) in Zika virus transmission: an integrative review

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