Zinc Blotting Assay for Detection of Zinc Binding Prolamin in Barley (Hordeum vulgare) Grain

Multi-method research strategy for understanding changes in barley grain protein composition and its relation to improved nutritional quality

“…Barley alcohol-soluble proteins (hordeins) were extracted from 50 mg of flour and the isolated proteins were separated on SDS-PAGE according to Uddin et al (2014 Kaczmarczyk et al (2012) Among the four hordein gene families, B-hordein was the most abundant transcript in both, parental and the transgenic lines, regardless the N dosage, representing up to 84.85%…”