Zinc finger transcription factor Sp7/Osterix acts on bone formation and regulates col10a1a expression in zebrafish

Niu, Pei; Zhong, Zhaomin; Wang, Mingyong; Huang, Guodong; Xu, Shuhao; Hou, Yi; Yan, Yi‐Lin; Wang, Han

doi:10.1016/j.scib.2017.01.009

Cited by 21 publications

(21 citation statements)

References 65 publications

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“…In teleost fish, the role of sp7 in bone and teeth has been recently reported (Kague et al, 2016;Niu et al;Yu et al, 2017;Zhong et al, 2016). We have described previously that zebrafish null for sp7 show severe defects in bone growth and mineralization, consistent with the roles ascribed to the gene through GWAS in humans, mouse and medaka fish.…”

Section: Introductionsupporting

confidence: 72%

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Kague

Witten

Soenens

et al. 2018

Preprint

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2 SummaryThe capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

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Section: Introductionsupporting

confidence: 72%

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Kague

Witten

Soenens

et al. 2018

Preprint

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“…Collagens are produced: collagen type I and collagen type II are the principal components of cartilage, minor collagens such as type X and XI help to stabilize the structure [ 19 ]. In zebrafish, col10a1a is an ortholog of mammalian Col10a1 encoding the alpha chain of type X collagen, a short collagen chain expressed in hypertrophic chondrocytes during endochondral ossification; in zebrafish it is a direct downstream target of osx [ 20 ].…”

Section: Skeletal Tissue and Bone Formationmentioning

confidence: 99%

“…In fact, in zebrafish osteoblasts, the Hh signaling pathway is an upstream inhibitor of atg5 gene, while the autophagic pathway inhibits osteoblast-related proteins Osterix, BMP2, and Col10a1 [ 21 ]. Furthermore, Shh (Sonic Hedgehog) stimulates osteoblastic differentiation and bone formation, upregulating Sp7 [ 21 ] which, in turn, directly upregulates col10a1 [ 20 ].…”

Section: Skeletal Tissue and Bone Formationmentioning

confidence: 99%

“…Importantly, a morpholino-mediated twist gene knockout in zebrafish has shown that both twist 1a and twist 1b but neither twist 2 nor twist 3 are crucial for correct skeletal development [ 16 ]. Niu et al applied the genome-editing tool TALEN to create a zebrafish sp7 mutant line, providing a useful tool to investigate the SP7 / Osterix gene [ 20 ]. The authors so demonstrated that sp7/osterix regulates col10a1a expression in zebrafish [ 20 ].…”

Section: Genes Involved In Bone Formation and Zebrafish Modelsmentioning

confidence: 99%

“…Niu et al applied the genome-editing tool TALEN to create a zebrafish sp7 mutant line, providing a useful tool to investigate the SP7 / Osterix gene [ 20 ]. The authors so demonstrated that sp7/osterix regulates col10a1a expression in zebrafish [ 20 ]. In addition, by applying the CRISPR/Cas9 genome-editing tool, two additional knockout SP7 lines were obtained which contributed to demonstrate that SP7 regulates dlx2b and bglap genes expression and, consequently, tooth development and mineralization [ 26 ].…”

Section: Genes Involved In Bone Formation and Zebrafish Modelsmentioning

confidence: 99%

See 2 more Smart Citations

Zebrafish: A Suitable Tool for the Study of Cell Signaling in Bone

Valenti

Marchetto

Mottes

et al. 2020

Cells

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In recent decades, many studies using the zebrafish model organism have been performed. Zebrafish, providing genetic mutants and reporter transgenic lines, enable a great number of studies aiming at the investigation of signaling pathways involved in the osteoarticular system and at the identification of therapeutic tools for bone diseases. In this review, we will discuss studies which demonstrate that many signaling pathways are highly conserved between mammals and teleost and that genes involved in mammalian bone differentiation have orthologs in zebrafish. We will also discuss as human diseases, such as osteogenesis imperfecta, osteoarthritis, osteoporosis and Gaucher disease can be investigated in the zebrafish model.

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Gene Editing Technology for Fish Health Management

Gupta

2021

Biotechnological Advances in Aquaculture Health Management

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Zinc finger transcription factor Sp7/Osterix acts on bone formation and regulates col10a1a expression in zebrafish

Cited by 21 publications

References 65 publications

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Zebrafish: A Suitable Tool for the Study of Cell Signaling in Bone

Gene Editing Technology for Fish Health Management

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