Zinc oxide nanocomb biosensor for glucose detection

Wang, J. X.; Sun, Xiao Wei; Wei, Anne; Yang, Lei; Cai, Xianpeng; Li, Chang Ming; Dong, Zhili

doi:10.1063/1.2210078

Cited by 568 publications

(328 citation statements)

References 24 publications

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“…3͑b͔͒ of the fabricated biosensor increases linearly ͑50-400 mg/ dL͒ with increasing glucose concentration with correlation coefficient ͑R͒ as 0.987 indicating good electrocatalytic behavior of GOx / CeO 2 / Au bioelectrode. Compared to similar glucose biosensors, [8][9][10] this sensor shows higher sensitivity ͑0.00287 A / mg dL −1 cm −2 ͒ and low detection limit ͑12.0 M͒.…”

Section: -mentioning

confidence: 99%

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Ansari

Solanki

Malhotra

2008

Applied Physics Letters

145

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Sol-gel derived nanostructured cerium oxide ͑CeO 2 ͒ film deposited on gold ͑Au͒ electrode has been utilized for physisorption of glucose oxidase ͑GOx͒. X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived CeO 2 / Au electrode and GOx / CeO 2 / Au bioelectrode. The response characteristics of the glucose bioelectrode ͑GOx / CeO 2 / Au͒ indicate linearity, detection limit and shelf-life as 50-400 mg/ dL, 12.0 M, and 12 weeks, respectively. The value of apparent Michaelis-Menten constant ͑K m ͒ of GOx / CeO 2 / Au bioelectrode has been found to be 13.55 M.

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Section: -mentioning

confidence: 99%

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Ansari

Solanki

Malhotra

2008

Applied Physics Letters

145

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“…[20][21][22][23][24][25][26] To summarize, we fabricated a glucose biosensor based on the combination of glucose oxidase and InN QDs. The presented biosensor utilizes the substantial advantages of high surface charge density and low-dimensionality of the InN QDs for highly sensitive and rapid response.…”

Section: -mentioning

confidence: 99%

Highly efficient potentiometric glucose biosensor based on functionalized InN quantum dots

Alvi¹,

Rodriguez

Gómez

et al. 2012

Applied Physics Letters

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We present a fast, highly sensitive, and efficient potentiometric glucose biosensor based on functionalized InN quantum-dots (QDs). The InN QDs are grown by molecular beam epitaxy. The InN QDs are bio-chemically functionalized through physical adsorption of glucose oxidase (GOD). GOD enzyme-coated InN QDs based biosensor exhibits excellent linear glucose concentration dependent electrochemical response against an Ag/AgCl reference electrode over a wide logarithmic glucose concentration range (1 x 10(-5) M to 1 x 10(-2) M) with a high sensitivity of 80mV/decade. It exhibits a fast response time of less than 2 s with good stability and reusability and shows negligible response to common interferents such as ascorbic acid and uric acid. The fabricated biosensor has full potential to be an attractive candidate for blood sugar concentration detection in clinical diagnoses. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4758701]

Funding Agencies|BBVA foundation||

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“…ZnO is a well-established material for bio-sensing applications due to its unique features such as high catalytic efficiency, bio-compatibility, strong adsorbance, electrochemical activity, easy availability and long term stability [1][2][3]. It has other advantages over other metal oxide thin films, including the possibility of low temperature deposition, facile fabrication of nanostructures and attractive optoelectronic and piezoelectric properties [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…Because of these well-known properties, GOx is an ideal model enzyme for studying the electron transfer pathway for new material interfaces such as ZnO/PC. Especially, ZnO has a high iso-electric point of 9.3, which provides a suitable interface for GOx with an iso-electric point of 4.5 [2]. As the GOx electron transfer mechanism depends on the distance between the catalytic site and the surface charges on the electrode, it was interesting to investigate how this may influence the reactivity of GOx immobilized on ZnO/PC or AZO/PC to glucose without a mediator.…”

Section: Introductionmentioning

confidence: 99%

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

V.T.K.P.

Inguva

Krishnamurthy

et al. 2017

Enzyme and Microbial Technology

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Research Highlights: Electrochemical interaction of ZnO and AZO thin films with the enzyme glucose oxidase was studied.  Nature of interaction of GOx with AZO was explored using XPS.  Mechanism of the electron transfer between GOx and AZO/ZnO was studied using EIS.  The results show that AZO is preferable to ZnO for GOx immobilization and glucose sensor development. Abstract:Al doped and undoped ZnO thin films were deposited by pulsed-laser deposition on polycarbonate sheets. The films were characterized by optical transmission, Hall effect measurement, XRD and SEM. Optical transmission and surface reflectometry studies showed good transparency with thicknesses ~100 nm and surface roughness of 10 nm. Hall effect measurements showed that the sheet carrier concentration was -1.44 x 10 15 cm -2 for AZO and -6 x 10 14 cm -2 for ZnO. The films were then modified by drop-casting glucose oxidase (GOx) without the use of any mediators. Higher protein concentration was observed on ZnO as compared to AZO with higher specific activity for ZnO (0.042 U mg -1 ) compared to AZO (0.032 U mg -1 ), and was in agreement with cyclic voltemmetry (CV). X-ray photoelectron spectroscopy (XPS) suggested that the protein was bound by dipole interactions between AZO lattice oxygen and the amino group of the enzyme. Chronoamperometry showed sensitivity of 5.5 µA mM -1 cm -2 towards glucose for GOx/AZO and 2.2 µA mM -1 cm -2 for GOx/ZnO. The limit of detection (LoD) was 167 µM of glucose for GOx/AZO, as compared to 360 µM for GOx/ZnO. The linearity was 0.28-28 mM for GOx/AZO whereas it was 0.6-28 mM for GOx/ZnO with a response time of 10s. Possibly due to higher enzyme loading, the decrease of impedance in presence of glucose was larger for GOx/ZnO as compared to GOx/AZO in electrochemical impedance spectroscopy (EIS). Analyses with clinical blood serum samples showed that the systems had good reproducibility and accuracy. The characteristics of novel ZnO and AZO thin films with GOx as a model enzyme, should prove useful for the future fabrication of inexpensive, highly sensitive, disposable electrochemical biosensors for high throughput diagnostics.

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Zinc oxide nanocomb biosensor for glucose detection

Cited by 568 publications

References 24 publications

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Sol-gel derived nanostructured cerium oxide film for glucose sensor

Highly efficient potentiometric glucose biosensor based on functionalized InN quantum dots

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports

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