Zinc Oxide Nanoparticles Induce DNA Damage in Sand Dollar Scaphechinus mirabilis Sperm

Kukla, Sergey Petrovich; Челомин, В. П.; Mazur, Andrey Alexandrovich; Слободскова, Валентина Владимировна

doi:10.3390/toxics10070348

Cited by 9 publications

(3 citation statements)

References 57 publications

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“…However, the reason for the antagonism between nAg and nCuO remains unclear. DNA damage was also increased in the sperm of the sand dollar Scaphechinux mirabilis exposed to zinc oxide nanoparticles and zinc ions indicating that DNA damage could be produced by both the nanoparticle and dissolved zinc [ 43 ]. However, dissolved Zn did not change with the treatment groups and was not correlated with DNA damage.…”

Section: Discussionmentioning

confidence: 99%

Investigation on the Toxicity of Nanoparticle Mixture in Rainbow Trout Juveniles

et al. 2023

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The environmental impacts of nanoparticle mixtures in the aquatic environment is not well understood. The purpose of this study examined the sub-lethal toxicity of low concentrations (ug/L range) of selected nanoparticles alone and in mixtures in juvenile trout. Fish were exposed to to individual and two environmentally relevant mixtures of silver (nAg), copper oxide (nCuO) and cerium oxide (nCeO) nanoparticles for 96 h at 15 °C. After the exposure period, fish were depurated overnight and tissue levels in Ag, Ce, Cu and Zn were determined along with a suite of effects biomarkers such as oxidative stress/inflammation, denatured protein tagging (ubiquitin), DNA strand breaks (genotoxicity) and acetylcholinesterase (AChE) activity. The data showed that these nanoparticles behaved as suspended matter but were nevertheless bioavailable for fish with bioconcentration factors of 6, 8 and 2 for nAg, nCeO and nCuO respectively. Only nCuO alone increased malonaldehyde (lipid peroxidation) contents but all nanoparticles increased DNA damage, protein-ubiquitin labeling, and decreased AChE activity. Globally, the toxicity of nCeO and nCuO was generally stronger than nAg, and antagonist effects were found in the mixtures. The interactions involved in these antagonisms are not well understood but do not involve the liberation of free ions and labile zinc in tissues. In conclusion, the bioavailability and toxicity of these nanoparticles are influenced by mixtures of nanoparticles, which is likely to occur in contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Investigation on the Toxicity of Nanoparticle Mixture in Rainbow Trout Juveniles

et al. 2023

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“…However, several studies in the literature reported that some biosynthesized ZnO NPs may have hazardous potential on living organisms and the environment (Dimkpa et al, 2012; Song et al, 2014; Zukiene and Snitka, 2015). Additionally, recent findings revealed genotoxic potentials of ZnO NPs, resulting in genomic instabilities and/or epigenetic alterations, which eventually translated into a variety of diseases (Akbaba and Türkez, 2018; Kukla et al, 2022; Srivastav et al, 2017). In this context, the genotoxic potentials of the NPs synthesized in our study were also investigated with the Ames/ Salmonella , E. coli WP2, Yeast DEL, seed germination, and RAPD assay systems.…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxicity assessment of biosynthesized zinc oxide nanoparticles

et al. 2023

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There are various studies on the toxicological potentials of conventionally synthesized zinc oxide (ZnO) nanoparticles, which are useful tools for many medical applications. However, knowledge about the biologically synthesized ones is still limited. In this study, the potential of producing ZnO nanoparticles via a green synthesis method, which enables safer, environmentally, economical and controlled production by using the Symphoricarpos albus L. plant, was investigated. For this purpose, aqueous extract was obtained from the fruits of the plant and reacted with zinc nitrate precursor. Characterization of the synthesized product was carried out by SEM and EDAX analyzes. In addition, the biosafety of the product was also investigated by using the Ames/ Salmonella, E. coli WP2, Yeast DEL, seed germination, and RAPD test systems. The results obtained from SEM studies showed that spherical nanoparticles with an average diameter of 30 nm were synthesized as a result of the reaction. EDAX findings confirmed that these nanoparticles were composed of Zn and O elements. On the other hand, according to the findings of the biocompatibility tests, the synthesized nanoparticle did not show any toxic and genotoxic effects up to a concentration of 640 μg/ml in any of the test systems. Accordingly, considering the findings of our study, it was concluded that the aqueous extract of S. albus fruits can be used for the green synthesis of ZnO nanoparticles, the products obtained successfully passed the biocompatibility tests in our study, and additionally, more comprehensive biocompatibility tests should be performed before industrial scale production.

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“…Reproductive and developmental toxicity are crucial components of overall toxicology and have received increasing attention in recent years . If exogenous substances are injected into the body of a male animal, it may adversely affect sperm quality, motility, and testicular morphology, thereby reducing fertility and affecting the health of offspring. − Several studies have demonstrated a decline in animal reproductive output after exposure to nickel, , gold nanoparticles, nano-titanium dioxide (TiO 2 ), − silicon dioxide (SiO 2 ) nanoparticles, − and carbon nanotubes …”

Section: Introductionmentioning

confidence: 99%

Reproductive Toxicity Evaluation of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes on Reproduction and Offspring Health

et al. 2023

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A new graphene-based fluorescent nanoprobe for tumor cell nucleus (GTTNs) was synthesized in our laboratory that penetrates the cell membrane and particularly targets cancer cell nucleus and displays tremendous potential for clinical applications. Although acute and subacute toxicity studies have been conducted on GTTNs, a primary result could be drawn that GTTNs appear to have almost no acute and subacute toxicity. However, as an important part of safety evaluation, the influences on reproductive and offspring developmental toxicity are still absent. In this study, male mice were injected intravenously with GTTNs, and the survival status, histopathology of the testes and epididymides, proliferation and apoptosis of testicular tissue, and sperm motility of mice were measured. To evaluate the short- and long-term fertility in male mice, different male mice resided with untreated female mice on days 1 and 30 after the end of the last treatment, and the offspring health parameters were assessed by measuring pup numbers, body weight, and organ indexes of the pups. The results indicated that GTTNs-exposed male mice retained good fertility, healthy structure of testes and epididymides, and production of healthy sperm. Meanwhile, there were no significant differences between the offspring and the control group. In consideration of GTTNs with broad prospects for biomedical applications, our results contribute a basis for further understanding of its biosafety.

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Zinc Oxide Nanoparticles Induce DNA Damage in Sand Dollar Scaphechinus mirabilis Sperm

Cited by 9 publications

References 57 publications

Investigation on the Toxicity of Nanoparticle Mixture in Rainbow Trout Juveniles

Investigation on the Toxicity of Nanoparticle Mixture in Rainbow Trout Juveniles

In vitro genotoxicity assessment of biosynthesized zinc oxide nanoparticles

Reproductive Toxicity Evaluation of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes on Reproduction and Offspring Health

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