Zinc promotes proliferation and activation of myogenic cells via the PI3K/Akt and ERK signaling cascade

Ohashi, Kazuya; Nagata, Yoichi; Wada, Eitaro; Zammit, Peter S.; Shiozuka, Masataka; Matsuda, Ryoichi

doi:10.1016/j.yexcr.2015.03.003

Cited by 83 publications

(74 citation statements)

References 40 publications

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“…[26] We show that NPs are not released into the culture medium at the timescale we work with, but they are available on the material surface as time progresses. These changes in surface physicochemical properties, while the presentation of the nanoparticle is taking place, is enough to trigger higher cellular processes, such as cell differentiation.…”

Section: Introductionmentioning

confidence: 77%

“…Nevertheless, differences observed were not statistically significant, which suggests that the release of zinc ions at this time was not enough to enhance cell proliferation, as has been suggested to happen with zinc concentrations within 25-50 µM. [26] Myoblast differentiation was assessed by staining for sarcomeric myosin that, together with multinucleated myotube formation, is characteristic of the differentiation process (Figure 6b, e). Four days is the standard time required for C2C12 in vitro differentiation studies with myogenic differentiation medium; after that time, the percentage of differentiation was similar between PLLA and PLLA/ZnO (Figure 6c).…”

Section: Cell Proliferation and Differentiationmentioning

confidence: 92%

“…[55,56] Conversely, the release of zinc into the culture medium, as PLLA degradation proceeds, is very low compared to the amounts needed to promote C2C12 differentiation (~ 3 µM in our system), which is further diluted due to the periodical changes of the medium during culture, compare to 25-50 µM. [26] For cell differentiation to occur, cells have to leave the cell cycle and hence, stop proliferation. After 4 days, the combination of the differentiation medium and the surface of PLLA are the modulators of myoblast differentiation, as it has been shown previously, [57] i.e.…”

Section: Cell Proliferation and Differentiationmentioning

confidence: 94%

“…[24,25] More recently has been shown that zinc promotes myoblast proliferation and differentiation via the activation of the ERK/Akt signalling cascade. [26] ZnO NPs have demonstrated their preferential ability to kill high proliferative cells, like cancer cells, versus normal cells. [19,27] In addition, the oxidative stress properties of ZnO NPs have been also tested; [28] ZnO NPs can be sequestered by autophagy and this internalization could generate reactive oxygen species (ROS) intracellularly.…”

Section: Introductionmentioning

confidence: 99%

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PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Trujillo

Lizundia

Vilas

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Graphical abstract2 Highlights  Biodegradable poly(L-lactide) matrix loaded with ZnO nanorods. Dynamic presentation of nanorods triggered by PLLA degradation. Changes in surface properties as a function of time control cell behaviour. Nanorods exposure promotes cell differentiation.3 Keywords: PLLA, ZnO nanoparticle, C2C12 myoblast, nanocomposite, cell differentiation AbstractThis work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ~ 20 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles -loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.4

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Section: Introductionmentioning

confidence: 77%

Section: Cell Proliferation and Differentiationmentioning

confidence: 92%

Section: Cell Proliferation and Differentiationmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Trujillo

Lizundia

Vilas

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…(48,59) or Nox silencing by controlling cyclin D1 level through Erk1/ 2, PI-3 kinase/AKT, and NF-jB pathways (223). Furthermore, it has recently been shown that zinc promotes the MuSC proliferation in a dose-dependent manner through the positive control of PI3K/Akt and ERK signaling pathways, although it is not known whether this occurs through a redoxdependent mechanism (244).…”

Section: Proliferationmentioning

confidence: 99%

Redox Control of Skeletal Muscle Regeneration

Moal

Pialoux

Juban

et al. 2017

Antioxidants & Redox Signaling

152

120

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Skeletal muscle shows high plasticity in response to external demand. Moreover, adult skeletal muscle is capable of complete regeneration after injury, due to the properties of muscle stem cells (MuSCs), the satellite cells, which follow a tightly regulated myogenic program to generate both new myofibers and new MuSCs for further needs. Although reactive oxygen species (ROS) and reactive nitrogen species (RNS) have long been associated with skeletal muscle physiology, their implication in the cell and molecular processes at work during muscle regeneration is more recent. This review focuses on redox regulation during skeletal muscle regeneration. An overview of the basics of ROS/RNS and antioxidant chemistry and biology occurring in skeletal muscle is first provided. Then, the comprehensive knowledge on redox regulation of MuSCs and their surrounding cell partners (macrophages, endothelial cells) during skeletal muscle regeneration is presented in normal muscle and in specific physiological (exercise-induced muscle damage, aging) and pathological (muscular dystrophies) contexts. Recent advances in the comprehension of these processes has led to the development of therapeutic assays using antioxidant supplementation, which result in inconsistent efficiency, underlying the need for new tools that are aimed at precisely deciphering and targeting ROS networks. This review should provide an overall insight of the redox regulation of skeletal muscle regeneration while highlighting the limits of the use of nonspecific antioxidants to improve muscle function. Antioxid. Redox Signal. 27, 276-310.

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WDR13 promotes the differentiation of bovine skeletal muscle‐derived satellite cells by affecting PI3K/AKT signaling

Tong

et al. 2019

Cell Biology International

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Muscle satellite cells are usually at rest, and when externally stimulated or regulated, they can be further differentiated by cell fusion to form new myotubes and muscle fibers. WD repeat domain 13 (WDR13) is highly conserved in vertebrates. Studies have shown that mice lacking the Wdr13 gene develop mild obesity, hyperinsulinemia, and increased islet β cell proliferation. However, the role of WDR13 in bovine cells is unclear. Here, we investigated the effect of WDR13 on bovine skeletal muscle‐derived satellite cells (MDSCs). We found that WDR13 was upregulated in bovine MDSCs using western blotting and immunofluorescence experiments. Moreover, activation and inhibition of WDR13 expression increased and decreased cell differentiation, respectively, suggesting that WDR13 promotes bovine MDSC differentiation. To further understand the mechanism of action of WDR13, we examined changes in the PI3K/AKT signaling pathway following WDR13 activation or inhibition. In addition, cells were treated with a phosphoinositide kinase 3 (PI3K) inhibitor, LY294004, to observe cell differentiation. The results showed that activation of WDR13 inhibited the PI3K/AKT signaling pathway and enhanced cell differentiation. These data suggest that WDR13 can promote the differentiation of bovine MDSCs by affecting the PI3K/AKT signaling pathway.

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Zinc promotes proliferation and activation of myogenic cells via the PI3K/Akt and ERK signaling cascade

Cited by 83 publications

References 40 publications

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Redox Control of Skeletal Muscle Regeneration

WDR13 promotes the differentiation of bovine skeletal muscle‐derived satellite cells by affecting PI3K/AKT signaling

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