Zn 2+ induced molecular responses associated with oxidative stress, DNA damage and histopathological lesions in liver and kidney of the fish, Channa punctatus (Bloch, 1793)

Ratn, Arun; Prasad, R Raghu; Awasthi, Yashika; Kumar, Manoj; Misra, Abha; Trivedi, Sunil P.

doi:10.1016/j.ecoenv.2017.12.058

Cited by 54 publications

(30 citation statements)

References 41 publications

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“…The ZnO in coatings also exerts antibacterial effects by ROS generation (i.e., H 2 O 2 , OH-, and O 2 2-) [50]. In addition, free Zn ions also penetrate bacterial membranes and cause DNA damage [51]. Research has proven that dental implant failure was commonly associated to dental plaque formation.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Biocompatibility and Antibacterial Activity of Selective Laser Melting Titanium with Zinc-Doped Micro-Nano Topography

et al. 2019

Journal of Nanomaterials

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Selective laser melting (SLM) titanium is a suitable material for use in customized implants. However, long-term implant survival requires both successful osseointegration and promising antibacterial characteristics. Native SLM titanium thus requires proper modifications to improve its bioactivity and antibacterial efficacy. Micro-arc oxidation (MAO) was conducted on sandblasted SLM substrate to form a microporous TiO2 coating. Subsequently, hydrothermal treatment was applied to fabricate micro-nano zinc-incorporated coatings with different Zn content (1 mM-Zn and 100 μM-Zn). Surface characterization was performed using scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, a three-dimensional profilometer, and a contact angle measuring device. The osteoblast-like cell line MC3T3-E1, Subclone 14, was used in cell viability assays to evaluate adhesion, proliferation, and ALP activity. An antibacterial assay was conducted using Streptococcus sanguinis and Fusobacterium nucleatum. Zn-incorporated samples exhibited higher adhesion, proliferation, and differentiation than did SLM and MAO samples. 100 μM Zn samples exhibited the highest proliferation, and 1 mM-Zn samples manifested the highest ALP activity. In addition, Zn-incorporated samples exerted inhibitory effects on both Streptococcus sanguinis and Fusobacterium nucleatum. Combining micro-arc oxidation and hydrothermal treatment, we successfully fabricated a novel Zn-incorporated coating on a microporous SLM surface which possesses both outstanding bioactivity and antibacterial efficacy.

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Section: Discussionmentioning

confidence: 99%

Enhanced Biocompatibility and Antibacterial Activity of Selective Laser Melting Titanium with Zinc-Doped Micro-Nano Topography

et al. 2019

Journal of Nanomaterials

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“…Journal of Environmental Biology, May 2021m ethods are employed for this purpose. Nevertheless, simple, reliable, sensitive, and cost-effective methods such as micronucleus test, chromosomal aberration test and DNA damage tests are still popular and being routinely applied to assess the genotoxicity of various chemicals in fishes (Pandey et al, 2011;Ratn et al, 2018;Yadav and Trivedi, 2009a).…”

Section: O N L I N E C O P ÿmentioning

confidence: 99%

“…Micronuclei induction is a sensitive indicator of DNA damage and can be effectively utilized to have sight of double strand breaks, single strand breaks, base mismatch and DNA adducts. This makes micronucleus test a reliable tool for evaluating genetic damage induced by aquatic pollutants in fishes and other aquatic organisms (Bolognesi, and Cirillo, 2014;Ratn et al, 2018;Yadav and Trivedi, 2009b). Chromosomal aberrations are important cyto-toxic end markers against a number of xenobiotics, including metals.…”

Section: O N L I N E C O P ÿmentioning

confidence: 99%

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Efficacy evaluation of Rauwolfia serpentina against Chromium (VI) toxicity in fish, Channa punctatus

Trivedi¹,

Kumar²,

Singh³

et al. 2021

JEB

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Aim: To explore the efficacy potential of ethanolic root extract of Rauwolfia serpentina against Chromium (VI) toxicity in fish, Channa punctatus. Methodology: Acclimatized fish were divided into six groups, each having 15 specimens. Group I served as a control, while Group II and III fish were exposed to ethanolic root extract of Rauwolfia serpentina (3 mg l-1) and Cr (VI) (96 h LC50/10; 7.68 mg l-1), respectively. Groups IV, V and VI fish were exposed to three different concentrations of ethanolic root extract of Rauwolfia serpentina (1, 2 and 3 mg l-1), simultaneously with 7.68 mg l-1 of Cr (VI). The induction of micronuclei, chromosomal aberrations, protein levels and liver enzymes-SGOT, SGPT and ALP- were assessed in fish of all six groups after designated exposure periods. Results: A significant induction (p<0.05) in chromosomal aberrations, micronuclei frequency and activities of liver enzymes (SGOT, SGPT and ALP) coupled with reduced protein level was recorded in Group III as compared to the control. Whereas, a significant (p<0.05) decrease in the frequency of chromosomal aberrations, micronuclei induction and activities of liver enzymes together with an increase in protein level was observed in Group IV, V and VI with respect to control and Group III. Interpretation: Present investigation evince the ameliorative potential of ethanolic root extract of Rauwolfia serpentina against Cr (VI) induced chromosomal aberrations and micronuclei induction, changes in protein levels and biochemical alterations of liver enzymes in Channa punctatus. Thus would help present work helps in saving the aquatic biodiversity and increasing the production of protein rich food fish.

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“…Furthermore, few reports are available about the molecular changes due to bisphenol A in liver, brain, kidneys, gills reproductive tissues of sh (Kumari and Khare, 2018;Wei et al, 2018). Hematological and biochemical parameters can be used as useful bio-indicators in sh and are well known targeted organs of toxicity after exposure to xenobiotics (Singh and Srivastava, 2010;Gul et al 2017;Ratn et al, 2018;Sisodiya et al, 2018). Moreover, estimation of variations in biochemical parameters such as protein, enzymes and glucose are commonly used to evaluate the physiological changes in aquatic environments to explain stress conditions (Abdel-Latif and Khashaba 2017; Abdel-Tawwab and Hamed, 2018).…”

Section: Introductionmentioning

confidence: 99%

New Insights Into Hematological, Serum Biochemical and Histopathological Toxicity of Bisphenol a on Bighead Carp (Aristichthys Nobils) Under Long-Term Exposure

Akram

Iqbal

Hussain

et al. 2021

Preprint

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Bisphenol A (BPA) is one of the highest volume chemicals produced worldwide and is frequently used in dental sealants, water bottles, food and beverage packaging. Due to persistant application, BPA has become potential threat to variety of organisms including public health. In this study for the first time 80 bighead carps were randomly placed in different four groups (A-D). Fish in groups (B-D) were treated with BPA 60 days while fish in group A served as control group. Body weight, absolute and relative weight of different visceral organs of fish exposed to higher concentrations (1500 µg/L) of BPA decreased significantly (p < .05). Results on proximate analysis showed significantly decreased in crude proteins, lipid contents and moisture contents in muscles while increased ash contents. Red blood cells count, hemoglobin concentration, lymphocytes and monocytes were significantly decreased while leukocytes counts and neutrophil counts was significantly increased in treated fish. Results on different serum biochemistry parameters like serum albumin and total proteins decreased significantly (p < .05) while alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), urea creatinine, glucose, cholesterol and lactate dehydrogenase (LDH) increased significantly (p < .05) in treated fish. Histopathological ailments like pyknosis, degeneration glomeruli, increased Bowman’s space, ceroid formation in kidneys while ceroid formation, hemorrhages, pyknosis, karyorrhexis, karyolysis, binucleated hepatocytes, nuclear hypertrophy and eccentric nuclei in liver were observed in treated fish. Histological observation of ndifferent sections of brain of treated fish exhibited degenerated neurons in cerebellum, lipofuscin deposition, microgliosis, necrotic neurons, inflammatory cell and severe hemorrhage. Results on light microscopic observation of different sections of heart of bighead carp revealed necrosis, inflammatory reaction, neutrophilic myocarditis and hemorrhages. In conclusion, it is suggested that BPA induces adverse effects on physical, blood-biochemical parameters and histopathological changes in multiple visceral tissues of exposed fish.

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Zn 2+ induced molecular responses associated with oxidative stress, DNA damage and histopathological lesions in liver and kidney of the fish, Channa punctatus (Bloch, 1793)

Cited by 54 publications

References 41 publications

Enhanced Biocompatibility and Antibacterial Activity of Selective Laser Melting Titanium with Zinc-Doped Micro-Nano Topography

Enhanced Biocompatibility and Antibacterial Activity of Selective Laser Melting Titanium with Zinc-Doped Micro-Nano Topography

Efficacy evaluation of Rauwolfia serpentina against Chromium (VI) toxicity in fish, Channa punctatus

New Insights Into Hematological, Serum Biochemical and Histopathological Toxicity of Bisphenol a on Bighead Carp (Aristichthys Nobils) Under Long-Term Exposure

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