Zoledronic acid decreases bone formation without causing osteocyte death in mice

The Role of Bisphosphonates in Multiple Myeloma: Mechanisms, Side Effects, and the Future

“…The presence of BPs in the osteocyte lacuna, however, is not enough to confirm the effect of the drug on these cells, and in vitro studies are controversial. Both prosurvival [27][28][29] and proapoptotic [30 -32] effects have been observed in vitro, and data in favor of increased bone deposition or impaired bone formation have been reported in vivo [33,34]. In vitro studies by Orriss et al [30] showed inhibition of OB viability and function at 100 nM and toxicity at concentrations Ͼ10 M. Similar effects were observed for Pam and Clo.…”

Preclinical evidence of potential craniofacial adverse effect of zoledronic acid in pediatric patients with bone malignancies

“…The craniofacial suture enlargement which represents the likely consequence of osteoblast differentiation and mineralization defect [29,30], and the domed form of the skull were gradually normalized. Moreover, the second and third molar eruptions were finally effective.…”

Low-magnitude high-frequency loading, by whole-body vibration, accelerates early implant osseointegration in ovariectomized rats

“…The indices included mineral apposition rate (MAR, mm/day) = distance of two labeled line/number of interval days; the ratio of mineralizing surface to bone surface (MS/BS, μm/μm) = [(0.5 single labeled perimeter + double labeled perimeter) × 100]/bone perimeter; and bone formation rate per unit of bone surface [BFR/BS, μm 3 /(μm 2 day)] = MS/BS × BMR (25,26). …”