α-Tocopherol modifies tyrosine phosphorylation and capacitation-like state of cryopreserved porcine sperm

Satorre, M.M.; Breininger, Elizabeth; Beconi, M.T.; Beorlegui, N.

doi:10.1016/j.theriogenology.2007.06.021

Cited by 61 publications

(46 citation statements)

References 53 publications

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“…Contrary with other studies, addition of antioxidants in the present concentrations in this study was ineffective in improving the longevity and survivability of the Kolbroek boar semen while the addition of antioxidants such as a α-tocopherol, butylated hydroxytoluene, superoxide dismutase, catalase, cysteine or glutathione in extenders to both cooled and frozen-thawed semen have been reported to improve the semen quality in boar (Funahashi and Sano, 2005;Breininger et al, 2005;Satorre et al, 2007), bull (Bilodeau et al, 2001a), turkey (Donoghue and Donoghue, 1997), stallion (Ball et al, 2001b) and ram (Uysal and Bucak, 2007).…”

Section: Viabilitycontrasting

confidence: 99%

“…Studies have demonstrated that seminal plasma also contains a number of enzymatic antioxidants such as superoxide dismutase (SOD, glutathione peroxidase/glutathione reductase (GPX/GRD) and catalase which protect the spermatozoa against lipid peroxidation. Antioxidants are believed to counteract the negative effects of lipid peroxidation hence posing a very positive chance of increasing motility, survivability and the functional membrane permeability (Satorre et al, 2007;Bilodeau et al, 2001b). Antioxidants such as L-cysteine for example play a role in the intracellular protective mechanism against oxidative stress as membrane stabiliser and capacitation inhibitor (Johnson et al, 2000).…”

Section: Viabilitymentioning

confidence: 99%

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Effect of antioxidants (taurine, cysteine, -tocopherol) on liquid preserved Kolbroek boar semen characteristics

Chatiza¹,

Mokwena²,

Nedambale³

et al. 2018

Afr. J. Biotechnol.

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Successful artificial insemination depends on maintaining longevity of semen in the reproductive tract, and semen characteristics among others. The study compared efficacy of Androhep extender supplemented with 75 mM taurine, 5 m M-cysteine, or 200 mg/l alpha-tocophero on liquid preservation of Kolbroek boar semen characteristics over 24 and 48 h after storage at 17°C. Total motility was evaluated using computer aided sperm analyzer (CASA), viability using SYBR/propidium iodidemembrane function using HOST test and lipid peroxidation using the MDA test. Statistical analysis was done using PROC GLM procedure of SAS (1996). Data were analyzed using analysis of variance (ANOVA). Untreated samples showed higher sperm quality in terms of membrane integrity, functionality and motility. All antioxidant treated spermatozoa did not show increased longevity (p>0.05) after 48 h of storage compared with untreated samples. Supplementation of Androhep extender with antioxidants did not improve sperm survivability of kolbroek liquid preserved semen. Therefore, the antioxidants were ineffective in improving longevity of Kolbroek liquid preserved semen after 48 h storage at 17°C. Further studies are required to find the effective antioxidant concentration to elicit sperm protection for Kolbroek boar semen liquid preservation.

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Section: Viabilitycontrasting

confidence: 99%

Section: Viabilitymentioning

confidence: 99%

Effect of antioxidants (taurine, cysteine, -tocopherol) on liquid preserved Kolbroek boar semen characteristics

Chatiza¹,

Mokwena²,

Nedambale³

et al. 2018

Afr. J. Biotechnol.

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“…Portanto, resultados divergentes com o uso de antioxidantes são comuns, pois podem estar relacionados ao tipo de antioxidante, ao meio diluidor, à concentração do antioxidante e à espécie animal (5,7,13,14) .…”

Section: Resultsunclassified

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

et al. 2017

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ResumoObjetivou-se determinar o efeito e o melhor nível de inclusão de ácido ascórbico em meio diluidor TRIS-Gema para criopreservação de sêmen caprino. Foram utilizados cinco ejaculados de três bodes da raça Anglo Nubiana. Cada ejaculado foi dividido em quatro alíquotas de 200 µL, compondo quatro tratamentos: um sem adição de ácido ascórbico (controle) e os demais com 0,0528; 0,1056 e 0,1584mg/mL de ácido ascórbico no diluidor TRIS-Gema. Avaliaram-se motilidade espermática progressiva e vigor espermático pós-diluição, pós-resfriamento e pós-descongelamento; integridade de membrana (HOST); integridade acrossomal e teste de termorresistência lento (TTR). Os dados foram submetidos à Análise de Regressão a 5% de probabilidade. Não houve diferença para motilidade progressiva e para vigor espermático, repectivamente, na pós-diluição; pós-resfriamento e pós-descongelamento, assim como para os testes complementares HOST e após 120 minutos do TTR, para motilidade e vigor, no pós-descongelamento (P>0,05). Houve comportamento quadrático com a inclusão do ácido ascórbico para integridade acrossomal (61,58%), com nível ótimo de 0,1006mg/mL de ácido ascórbico (P<0,05). A inclusão de 0,1006mg/mL de ácido ascórbico no diluidor TRIS-Gema melhorou a integridade acrossomal durante o processo de criopreservação, podendo ser uma alternativa na composição dos diluidores seminais caprinos. Palavras-chave: antioxidantes; espermatozoide; estresse oxidativo; TRIS-gema. AbstractThe objective of this study was to determine the optimal inclusion level of ascorbic acid in TRISyolk extenders for cryopreservation of goat semen. We used five ejaculates of three Anglo Nubian goats. Semen from each animal was divided into four 200μL aliquots, composing four treatments: no

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“…Once the sample was considered suitable for freezing by presenting less than 3.5 crosses on masal motility and 80% of individual motility (Caballero et al, 2004;Satorre et al, 2007), freezing was made place, following the method pointed by Sellos (2001). For the freezing process, in all treatments, it was considered to add the corresponding LEY volume for reaching a final concentration of 1500 million sperm cells mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Osmolarity of Coconut Water (Cocos nucifera) Based Diluents and their Effect Over Viability of Frozen Boar Semen

Luzardo¹,

Gamboa²,

López³

et al. 2010

American J. of Animal and Veterinary Sciences

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Problem statement: Boar sperm cells are sensitive to the freezing process, which compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing sperm cell damage caused by the temperature and osmolarity changes during the freezing process, crioprotective and antioxidant substances have been added to the freezing media, such as coconut water, in order to increase the viability of frozen-thawed swine semen. The addition of any substance to the freezing diluent, directly affects osmolarity of the media, which can have positive or negative effects over the sperm cell. Approach: There are no published studies currently that indicate the effect of adding coconut water over the osmolarity of freezing media and their effect over viability of sperm cells, therefore, the objective of the present study was to evaluate the effect of coconut water based diluents osmolarity over the Motility (Mot), Acrosome Integrity (AI), Membrane Integrity (MI) and Mitochondrial Activity (MA) of thawed boar sperm cells. The treatments used were control T1 (LEY with bidistilled water + LEYGO) with an osmolarity range of 296-368 mOsmol Kg −1 , T2 (LEY and deionized coconut water + LEYGO) between 381 and 480 mOsmol Kg −1 and T3 (LEY and in natura coconut water + LEYGO) between 519 and 1041 mOsmol Kg −1 . The Westendorf modified method was the freezing method used. The obtained data were statistically analyzed by GLM, using the SAS software (SAS, 2000). Results: A significant difference was observed on T2 compared to T1 regarding Mot 41.9 Vs 36.9% and MI 58.0 Vs 50.2. T3 had a detrimental effect on all studied variables. Conclusion: Under our study conditions, the osmolarity range of T2, due to the non ionic solutes content, contributed to improve the viability of frozen-thawed sperm cells.

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α-Tocopherol modifies tyrosine phosphorylation and capacitation-like state of cryopreserved porcine sperm

Cited by 61 publications

References 53 publications

Effect of antioxidants (taurine, cysteine, -tocopherol) on liquid preserved Kolbroek boar semen characteristics

Effect of antioxidants (taurine, cysteine, -tocopherol) on liquid preserved Kolbroek boar semen characteristics

Ácido Ascórbico No Diluidor Para Criopreservação De Sêmen Caprino

Osmolarity of Coconut Water (Cocos nucifera) Based Diluents and their Effect Over Viability of Frozen Boar Semen

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