α‐tocopherol pretreatment alleviates cerebral ischemia‐reperfusion injury in rats

Lv, Shitao; Yang, Haiyan; Jing, Pengcheng; Song, Haiying

doi:10.1111/cns.13814

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…20,31 Second, the role of specific glial cell surface molecules in lymphatic flow is investigated by molecular biology methods such as gene editing and protein labeling. 20,32 It can also be combined with immunohistochemistry to observe the distribution of cerebrospinal fluid tracers and the expression of specific molecules. 33 Finally, neurobehavioral experiments were used to assess the effects of abnormal glymphatic system function on animal behavior, such as cognition and emotion regulation.…”

Section: Research Methods Of the Glymphatic Systemmentioning

confidence: 99%

“…First, using advanced microscopic imaging techniques and magnetic resonance imaging, 30 such as two‐photon microscopy, researchers can observe in real time the dynamic processes of astrocytes and the flow patterns of cerebrospinal fluid and interstitial fluid 20,31 . Second, the role of specific glial cell surface molecules in lymphatic flow is investigated by molecular biology methods such as gene editing and protein labeling 20,32 . It can also be combined with immunohistochemistry to observe the distribution of cerebrospinal fluid tracers and the expression of specific molecules 33 .…”

Section: Glymphatic Systemmentioning

confidence: 99%

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Advances in the study of the glymphatic system and aging

Xiong,

Yu,

Zhi

et al. 2024

CNS Neurosci Ther

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The glymphatic system is cerebrospinal fluid–brain tissue fluid exchange flow mediated by aquaporin‐4 (AQP4) on the end feet of astrocytes for a system, which is capable of rapidly removing brain metabolites and thus maintaining brain homeostasis, and is known as the central immune system. Dysfunction of the glymphatic system causes accumulation of misfolded and highly phosphorylated proteins (amyloid‐β and Tau proteins), which destabilizes the proteins, and the body's neuroinflammatory factors are altered causing aging of the immune system and leading to neurodegenerative diseases. Damage to the glymphatic system and aging share common manifestations, as well as unstudied biological mechanisms that are also linked, such as mitochondria, oxidative stress, chronic inflammation, and sleep. In this paper, we first summarize the structure, function, and research methods of the glymphatic system and the relationship between the glymphatic system and the peripheral immune system, and second, sort out and summarize the factors of the glymphatic system in removing metabolites and resolving aging‐related diseases and factors affecting aging, to explore its related biological mechanisms, and moreover, to provide a new way of thinking for treating or intervening aging‐related diseases.

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Section: Research Methods Of the Glymphatic Systemmentioning

confidence: 99%

Section: Glymphatic Systemmentioning

confidence: 99%

Advances in the study of the glymphatic system and aging

Xiong,

Yu,

Zhi

et al. 2024

CNS Neurosci Ther

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“…The methodology for conducting western blot analysis was guided by previously published literature. 23,24 In brief, the procedure involves the collection of protein samples derived from damaged brain tissue, followed by protein electrophoresis and PVDF membrane transfer.…”

Section: Western Blotmentioning

confidence: 99%

CD4⁺CD11b⁺ T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling

Jiang,

Liu,

Chen

et al. 2024

CNS Neurosci Ther

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AimWe aim to identify the specific CD4+ T‐cell subtype influenced by brain‐to‐CLN signaling and explore their role during the acute phase of traumatic brain injury (TBI).MethodCervical lymphadenectomy or cervical afferent lymphatic ligation was performed before TBI. Cytokine array and western blot were used to detect cytokines, while the motor function was assessed using mNss and rotarod test. CD4+ T‐cell subtypes in blood, brain, and CLNs were analyzed with Cytometry by time‐of‐flight analysis (CyTOF) or fluorescence‐activated cell sorting (FACS). Brain edema and volume changes were measured by 9.4T MRI. Neuronal apoptosis was evaluated by terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining.ResultsCervical lymphadenectomy and ligation of cervical lymphatic vessels resulted in a decreased infiltration of CD4+ T cells, specifically CD11b‐positive CD4+ T cells, within the affected region. The population of CD4+CD11b+ T cells increased in ligated CLNs, accompanied by a decrease in the average fluorescence intensity of sphingosine‐1‐phosphate receptor‐1 (S1PR1) on these cells. Administration of CD4+CD11b+ T cells sorted from CLNs into the lateral ventricle reversed the attenuated neurologic deficits, brain edema, and lesion volume following cervical lymphadenectomy.ConclusionThe infiltration of CD4+CD11b+ T cells exacerbates secondary brain damage in TBI, and this process is modulated by brain‐to‐CLN signaling.

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“… 1 Nowadays, immediate restoration of the blood supply is the main strategy against IS, while it may cause the brain injury for sake of CI/R. 2 Indeed, the therapeutic effects of surgery and drug administration against CI/R remain not ideal. Therefore, it is essential to find novel strategies against CI/R injury.…”

Section: Introductionmentioning

confidence: 99%

Exosomal miR-133a-3p Derived from BMSCs Alleviates Cerebral Ischemia-Reperfusion Injury via Targeting DAPK2

Yang¹,

Xu²,

Lan³

et al. 2023

IJN

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Background: Cerebral ischemia-reperfusion (CI/R) injury is a subtype of complication after treatment of ischemic stroke. It has been reported that exosomes derived from BMSCs could play an important role in CI/R injury. However, whether BMSCs-derived exosomes could regulate CI/R injury via carrying miRNAs remains to be further explored. Methods: RNA sequencing was performed to identify the differentially expressed miRNAs. To mimic CI/R in vitro, SH-SY5Y cells were exposed to oxygen glucose deprivation/reoxygenation (OGD/R). The viability of SH-SY5Y cells was tested by CCK8 assay, and TUNEL staining was performed to detect the cell apoptosis. Results: MiR-133a-3p was identified to be reduced in exosomes derived from the plasma of patients with IS. Upregulation of miR-133a-3p significantly reversed OGD/R-induced SH-SY5Y cell growth inhibition. Consistently, BMSCs-derived exosomal miR-133a-3p could restore OGD/R-decreased SH-SY5Y cell proliferation via inhibiting apoptosis. Meanwhile, DAPK2 was a direct target of miR-133a-3p. In addition, OGD/R notably upregulated the level of DAPK2 and weakened the expressions of p-Akt and p-mTOR in SH-SY5Y cells, whereas exosomal miR-133a-3p derived from BMSCs notably reversed these phenomena. Exosomal miR-133a-3p derived from BMSCs could reverse OGD/R-induced cell apoptosis via inhibiting autophagy. Furthermore, exosomal miR-133a-3p derived from BMSCs markedly alleviated the symptom of CI/R injury in vivo. Conclusion: Exosomal miR-133a-3p derived from BMSCs alleviates CI/R injury via targeting DAPK2/Akt signaling. Thus, our study might shed new light on discovering new strategies against CI/R injury.

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α‐tocopherol pretreatment alleviates cerebral ischemia‐reperfusion injury in rats

Abstract: α‐tocopherol showed antioxidant, anti‐inflammatory and anti‐apoptotic abilities in rat brain tissue, thus alleviating cerebral ischemia‐reperfusion injury‐induced nerve damage.

Cited by 5 publications

References 33 publications

Advances in the study of the glymphatic system and aging

Advances in the study of the glymphatic system and aging

CD4⁺CD11b⁺ T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling

Exosomal miR-133a-3p Derived from BMSCs Alleviates Cerebral Ischemia-Reperfusion Injury via Targeting DAPK2

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α‐tocopherol pretreatment alleviates cerebral ischemia‐reperfusion injury in rats

Abstract: α‐tocopherol showed antioxidant, anti‐inflammatory and anti‐apoptotic abilities in rat brain tissue, thus alleviating cerebral ischemia‐reperfusion injury‐induced nerve damage.

Cited by 5 publications

References 33 publications

Advances in the study of the glymphatic system and aging

Advances in the study of the glymphatic system and aging

CD4+CD11b+ T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling

Exosomal miR-133a-3p Derived from BMSCs Alleviates Cerebral Ischemia-Reperfusion Injury via Targeting DAPK2

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CD4⁺CD11b⁺ T cells infiltrate and aggravate the traumatic brain injury depending on brain‐to‐cervical lymph node signaling