β-Adrenergic-cyclic AMP signalling pathway modulates cell function at the transcriptional level in 3T3-F442A adipocytes

Antras, J; Lasnier, Françoise; Pairault, Jacques

doi:10.1016/0303-7207(91)90030-v

Cited by 42 publications

(35 citation statements)

References 52 publications

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“…We did not identify any line speci®c activity of the Ap2 gene, which was extremely different between individuals within every line. In contrast to GDC-1 and LPL, for which the idea of a coordinate induction or suppression of the expression during differentiation and by hormonal stimuli in vitro 4,15,41 is in line with our observations, no such relation was applicable to Ap2.…”

Section: Discussionsupporting

confidence: 76%

“…Among these candidate genes putatively in¯uencing the complex quantitative traits are those encoding lipoprotein lipase (Lpl, chr 8), glycerolphosphate dehydrogenase 1 (GDC-1, chr 15), and adipocyte protein 2 (Ap2, chr 3), which control crucial steps in fatty acids uptake in adipocytes, triglyceride synthesis and intracellular lipid storage. These genes have expression patterns characteristic for cell maturity and metabolic condition, and are subject to a complex regulation by hormones and second messengers, in particular, by insulin, glucocorticoids and cAMP, as shown with cultured cells 15,16 and for in vivo models. 17 If these genes contribute to obesity, this is due to a DNA sequence variation affecting expression or function.…”

Section: Introductionmentioning

confidence: 99%

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Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Timtchenko

Kratzsch

Sauerwein³

et al. 1999

Int J Obes

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OBJECTIVE: For a detailed understanding of the complex traits growth and fat storage, a dissection into single genetic entities is mandatory. Therefore, blood plasma concentrations of hormones and the expression of selected genes were measured in extremely differentiated mouse lines. Genes were selected as candidates which might in¯uence the complex traits body weight and fat accumulation, and which are located in chromosomal regions recently identi®ed to affect trait differences between the lines. SUBJECTS AND MEASUREMENTS: The mouse lines were selected for high body weight (DU6), high carcass protein content (DU6P) and unselected controls (DUKs). In the selected lines DU6 and DU6P, mean body weights at the age of six weeks were about twice as high as the DUKs, whereas total fat weight was increased 2.2-fold in DU6 mice compared to DU6P and 3.2-fold in comparison to DUKs. Blood plasma concentrations of insulin-like growth factor 1 (IGF-1), growth hormone (GH), insulin and leptin, were measured in all lines at three weeks and at six weeks of age. Expression patterns of the genes encoding growth hormone (Gh), insulin-like growth factor 1 (Igf1), lipoprotein lipase (Lpl), glycerolphosphate dehydrogenase 1 (GDC-1), and adipocyte protein 2 (Ap2) were analyzed by Northern blot hybridization. RESULTS: In line DU6, highly signi®cant increased concentrations of insulin and leptin were observed at six weeks of age; at this stage, IGF-1 concentrations were elevated in the two selected lines compared to controls with maximal concentrations of IGF-1 and GH in DU6P. The amount of mRNA for GH in the pituitary gland, for Igf1 in the liver and for LPL in epididymal fat tissue was signi®cantly elevated in the two selected lines compared to controls at the age of three weeks, but not at six weeks. IGF-1 and GDC-1 mRNA concentrations were signi®cantly higher in the DU6 mice than in the DU6P (P`0.01) and the DUKs (P`0.001) mice examined at both ages. CONCLUSIONS: The results prove line-speci®c concentrations of the analyzed hormones and the transcription amounts of Gh, Igf1, GDC-1 and Lpl. The measured differences are either direct genetic effects or secondary changes, resulting from different food consumption.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Timtchenko

Kratzsch

Sauerwein³

et al. 1999

Int J Obes

View full text Add to dashboard Cite

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“…serum-free media) for 24 h prior to the addition of IL-6, forskolin, or inhibitors. After incubation with these compounds, whole-cell lysates were prepared as described previously (39). Equal amounts of protein (40 g) from each sample were electrophoresed on SDS-PAGE (8 or 10%) followed by transfer to a nitrocellulose membrane for Western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

Activation of the Androgen Receptor N-terminal Domain by Interleukin-6 via MAPK and STAT3 Signal Transduction Pathways

Ueda

Bruchovsky

Sadar

2002

Journal of Biological Chemistry

348

336

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“…In murine 3T3-L1 adipocytes, an AP-1-like sequence (Ϫ1,856 to Ϫ1,850) was responsible for the inhibition of LPL gene transcription by estrogen (100). Transcription of the LPL gene increases during adipocyte differentiation (101), and decreases in mature adipocytes after treatment with TNF-␣ (102, 103) and agents that increase cAMP (104,105). In the mouse, the inhibition of LPL gene transcription by TNF-␣ is secondary to the binding of at least two DNA binding proteins, NF-Y to the CCAAT box, and an octamer binding protein other than Oct-1 (102).…”

Section: Lpl Gene Transcriptionmentioning

confidence: 99%

Lipoprotein lipase

Merkel

Eckel

Goldberg

2002

Journal of Lipid Research

464

113

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Lipoprotein lipase (LPL) regulates the plasma levels of triglyceride and HDL. Three aspects are reviewed. 1 ) Clinical implications of human LPL gene variations: common mutations and their effects on plasma lipids and coronary heart disease are discussed. 2 ) LPL actions in the nervous system, liver, and heart: the discussion focuses on LPL and tissue lipid uptake. 3 ) LPL gene regulation: the LPL promoter and its regulatory elements are described. -Merkel, M., R. H. Eckel, and I. J. Goldberg. Lipoprotein lipase: genetics, lipid uptake, and regulation.

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β-Adrenergic-cyclic AMP signalling pathway modulates cell function at the transcriptional level in 3T3-F442A adipocytes

Cited by 42 publications

References 52 publications

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Activation of the Androgen Receptor N-terminal Domain by Interleukin-6 via MAPK and STAT3 Signal Transduction Pathways

Lipoprotein lipase

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