β-amyrin as an analgesic component of the leaves of Callistemon citrinus (Curtis) Skeels: Chemical, biological and in silico studies

Ahmed, Farhana; Rahman, Mohammed Z.; Khan, Mohammad Firoz; Rashid, Mohammad A.; Rahman, Mohammad Sharifur

doi:10.3329/bjb.v48i2.47685

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Prostaglandin is subsequently metabolized by the downstream tissue-specific synthases into potent signaling molecules that play fundamental roles in both the regulation of physiological homeostasis as well as in disease states such as nociceptive inflammation and cancer [45]. The compounds 1-3 are known for their potential analgesic activity [26][27][28] and have shown a high binding affinity and in silico inhibitory activity against COX-2 [29 -31]. In this study, the binding efficiency of the 3 ,4 ,6 -triacetylated pentanoic acid glucoside (compound 4) was calculated.…”

Section: Docking Studies For Analgesic Activitymentioning

confidence: 99%

“…Furthermore, stigmasterol and β-sitosterol have been shown to inhibit intraperitoneal acetic acid-induced chemical nociception [26,27]. Moreover, in a mouse model, β-amyrin was shown to possess peripheral analgesia and anti-inflammatory membrane stabilizing activity [28]. These compounds also showed a high binding affinity, and in silico inhibitory activity against cyclooxygenase 2 (COX-2) receptor-substrate [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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In Vivo and In Silico Analgesic Activity of Ficus populifolia Extract Containing 2-O-β-D-(3′,4′,6′-Tri-acetyl)-glucopyranosyl-3-methyl Pentanoic Acid

Mohammed

Khan

2023

IJMS

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Natural product-based structural templates have immensely shaped small molecule drug discovery, and new biogenic natural products have randomly provided the leads and molecular targets in anti-analgesic activity spheres. Pain relief achieved through opiates and non-steroidal anti-inflammatory drugs (NSAIDs) has been under constant scrutiny owing to their tolerance, dependency, and other organs toxicities and tissue damage, including harm to the gastrointestinal tract (GIT) and renal tissues. A new, 3′,4′,6′-triacetylated-glucoside, 2-O-β-D-(3′,4′,6′-tri-acetyl)-glucopyranosyl-3-methyl pentanoic acid was obtained from Ficus populifolia, and characterized through a detailed NMR spectroscopic analysis, i.e., 1H-NMR, 13C-DEPT-135, and the 2D nuclear magnetic resonance (NMR) correlations. The product was in silico investigated for its analgesic prowess, COX-2 binding feasibility and scores, drug likeliness, ADMET (absorption, distribution, metabolism, excretion, and toxicity) properties, possible biosystem’s toxicity using the Discovery Studio®, and other molecular studies computational software programs. The glycosidic product showed strong potential as an analgesic agent. However, an in vivo evaluation, though at strong levels of pain-relieving action, was estimated on the compound’s extract owing to the quantity and yield issues of the glycosidic product. Nonetheless, the F. populifolia extract showed the analgesic potency in eight-week-old male mice on day seven of the administration of the extract’s dose in acetic acid-induced writhing and hot-plate methods. Acetic acid-induced abdominal writhing for all the treated groups decreased significantly (p < 0.0001), as compared to the control group (n = 6) by 62.9%, 67.9%, and 70.9% of a dose of 100 mg/kg (n = 6), 200 mg/kg (n = 6), and 400 mg/kg (n = 6), respectively. Similarly, using the analgesia meter, the reaction time to pain sensation increased significantly (p < 0.0001), as compared to the control (n = 6). The findings indicated peripheral and central-nervous-system-mediated analgesic action of the product obtained from the corresponding extract.

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Section: Docking Studies For Analgesic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vivo and In Silico Analgesic Activity of Ficus populifolia Extract Containing 2-O-β-D-(3′,4′,6′-Tri-acetyl)-glucopyranosyl-3-methyl Pentanoic Acid

Mohammed

Khan

2023

IJMS

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Pharmacological action and underlying molecular mechanism of Callistemon: A genus of promising medicinal herbs

Rathore

Rai²

2022

Phytomedicine

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Characterization by LC-MS/MS and Antioxidant Activity of Extract and its Different Fractions of Calotropis procera Young Leaves

Agrawal,

Murti

2024

Scopus Indexed

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Objective: Plants are extremely useful as a source of medication. Calotropis procera is a plant that is used to treat a variety of illnesses in traditional medical and folklore systems. In this work, bioassay guided liquid-liquid fractionation, phytochemical and molecular spectroscopic analysis and the antioxidant activity of young leaves of Calotropis procera were evaluated. Methods: Bioassay-guided fractionation was carried out on the basis of polarity of solvents and UV, FTIR, LCMS profiling were conducted by using the standard methods. The DPPH scavenging assay was used to determine the antioxidant activity. Results: The extractive value for the ethyl acetate fraction was 9.14 % w/w. The Total phenolic content was highest in the water fraction 83.63±0.0788 GAE/g of dry weight of extract. UV spectroscopy was used to standardize the fractions. The Presence of various functional groups in the extract/fraction was confirmed by FTIR spectroscopy. Based on LCMS, fifty compounds were identified in the hydro-ethanolic extract/fractions. Among all the fractions, the chloroform fraction showed the greatest antioxidant properties (IC50 values=8.03 mg/mL). Conclusion: On the basis of the result of the study it can be concluded that Calotropis procera have the potential to become a promising herb for the treatment of various ailments.

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β-amyrin as an analgesic component of the leaves of Callistemon citrinus (Curtis) Skeels: Chemical, biological and in silico studies

Cited by 3 publications

References 16 publications

In Vivo and In Silico Analgesic Activity of Ficus populifolia Extract Containing 2-O-β-D-(3′,4′,6′-Tri-acetyl)-glucopyranosyl-3-methyl Pentanoic Acid

In Vivo and In Silico Analgesic Activity of Ficus populifolia Extract Containing 2-O-β-D-(3′,4′,6′-Tri-acetyl)-glucopyranosyl-3-methyl Pentanoic Acid

Pharmacological action and underlying molecular mechanism of Callistemon: A genus of promising medicinal herbs

Characterization by LC-MS/MS and Antioxidant Activity of Extract and its Different Fractions of Calotropis procera Young Leaves

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