β-asarone induces cell apoptosis, inhibits cell proliferation and decreases migration and invasion of glioma cells

Wang, Nanbu; Han, Yufeng; Luo, Lan; Zhang, Qinxin; Ning, Baile; Fang, Yongqi

doi:10.1016/j.biopha.2018.06.169

Cited by 14 publications

(5 citation statements)

References 21 publications

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“…For instance, β-asarone exerts antifungal and anthelmintic activity, regulates the nervous system and blocks cholesterol synthesis (27)(28)(29). In addition, previous studies have also highlighted the inhibitory function of β-asarone on tumor metastasis in glioma, lung cancer and gastric cancer cells (10)(11)(12). Furthermore, the antiproliferative property of β-asarone has been reported in gastric cancer and LoVo colon cancer cells in a previous study (13,14).…”

Section: Discussionmentioning

confidence: 80%

“…The authors' previous study demonstrated that modified Si-Jun-Zi Decoction (SJZ), a Chinese medicinal formula, could inhibit colon cancer liver metastasis by increasing the content of macrophage cells (9). A second Chinese medicine, acorus calamus, or its bioactive phytochemical β-asarone, has been widely reported to possess antitumor and chemopreventive activities in multiple carcinomas, including lung cancer (10), gastric cancer (11) and glioma (12). The suppressive role of β-asarone in gastric cancer cell proliferation has also been indicated (13).…”

Section: Introductionmentioning

confidence: 99%

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β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

Chen

Zhuang

et al. 2021

Oncol Lett

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Studies have revealed that β-asarone exerts a powerful inhibitory effect on the proliferation of human cancer cells. The authors' previous study demonstrated that β-asarone could induce LoVo colon cancer cell apoptosis in vitro and in vivo, indicating its anticancer properties. The present study aimed to determine the antineoplastic effect of β-asarone in HCT116 colon cancer cells. An in vitro proliferation assay using a real time cell analyzer demonstrated that β-asarone effectively decreased HCT116 cell proliferation in a dose-dependent manner. Bioinformatics analysis revealed that differentially expressed genes following β-asarone inhibition were involved in the 'cell cycle', 'cell division', 'cell proliferation' and 'apoptosis'. Subsequently, a xenograft assay evidenced the inhibitory effect of β-asarone on the growth of HCT116 tumors in vivo. Further detection of immune-associated cytokines and cells suggested that β-asarone might be involved in the antitumor immune response by stimulating granulocyte-colony stimulating factor and increasing the number of macrophage cells in the spleen. Additionally, a murine model of splenic-transplantation verified the strong suppressive role of β-asarone in colon cancer liver metastasis in vivo. Taken together, the results of the current study revealed that β-asarone decreased HCT116 colon cancer cell proliferation and liver metastasis potentially by activating the innate immune system, supporting the multi-system regulation theory and providing a basis for further mechanistic studies on colon cancer.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

Chen

Zhuang

et al. 2021

Oncol Lett

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“…Cancer cells can proliferate without limitation because of the loss of cell-cycle regulation, 41 and modern research has aimed to develop novel drugs or adjuvants for cancer treatments by inducing cell apoptosis. Mitochondria can trigger the apoptosis of tumor cells, 42 resulting in a series of apoptotic indicators appearing, including cell shrinkage and nuclear fragmentation.…”

Section: Discussionmentioning

confidence: 99%

Selenium-Modified Chitosan Induces HepG2 Cell Apoptosis and Differential Protein Analysis

Sun¹,

Deng²,

Peng³

et al. 2022

CMAR

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Introduction:Chitosan is the product of the natural polysaccharide chitin removing part of the acetyl group, and exhibits various physiological and bioactive functions. Selenium modification has been proved to further enhance the chitosan bioactivities, and has been a hot topic recently. Methods: The present study aimed to investigate the potential inhibitory mechanism of selenium-modified chitosan (SMC) on HepG2 cells through MTT assays, morphological observation, annexin V-FITC/PI double staining, mitochondrial membrane potential determination, cell-cycle detection, Western blotting, and two-dimensional gel electrophoresis (2-DE). Results:The results indicated that SMC can induce HepG2 cell apoptosis with the cell cycle arrested in the S and G 2 /M phases and gradual disruption of mitochondrial membrane potential, reduce the expression of Bcl2, and improve the expression of Bax, cytochrome C, cleaved caspase 9, and cleaved caspase 3. Also, 2-DE results showed that tubulin α 1 B chain, myosin regulatory light chain 12A, calmodulin, UPF0568 protein chromosome 14 open reading frame 166, and the cytochrome C oxidase subunit 5B of HepG2 cells were downregulated in HepG2 cells after SMC treatment. Discussion: These data suggested that HepG2 cells induced apoptosis after SMC treatment via blocking the cell cycle in the S and G 2 /M phases, which might be mediated through the mitochondrial apoptotic pathway. These results could be of benefit to future practical applications of SMC in the food and drug fields.

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“…Consistently, β-asarone treatment suppresses neuronal apoptosis through CaMKII/CREB/Bcl-2 pathway in Alzheimer disease models [ 29 ]. Moreover, it can induce apoptosis of glioma cells [ 30 ]. Thus, β-asarone could attenuate neuronal apoptosis in ischemic stroke.…”

Section: Discussionmentioning

confidence: 99%

Effects of β-Asarone on Ischemic Stroke in Middle Cerebral Artery Occlusion Rats by an Nrf2-Antioxidant Response Elements (ARE) Pathway-Dependent Mechanism

Pan

Cai

et al. 2021

Med Sci Monit

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Background This study assessed the effects and underlying molecular mechanisms of β-asarone on ischemic stroke model rats. Material/Methods Ischemic stroke was induced by middle cerebral artery occlusion (MCAO) in rats. Before and after modeling, cognitive function was evaluated via fear conditioning test and neurological deficit was determined via Longa and Bederson scores. Following treatment with β-asarone or nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor for 20 consecutive days, the cerebral infarction was detected via TTC staining and Cresyl Violet staining in brain tissues. TUNEL staining and western blot analysis for apoptosis-related proteins were performed to assess the apoptosis of neurons. Nrf2-antioxidant response elements (ARE) pathway-related proteins were examined by RT-qPCR or western blot. Results The cognitive and neurological function was defective in MCAO rats. The infarction volumes and the apoptosis of cortical neurons were significantly increased in brain tissues of model rats, which were ameliorated after treatment with β-asarone. Meanwhile, the increase in pro-apoptotic proteins and decrease in anti-apoptotic proteins were found in brain tissues of model rats, which were markedly ameliorated by β-asarone treatment. However, Nrf2 inhibitor worsened the cerebral infarction and the apoptosis of neurons. Western blot results showed that β-asarone treatment activated the Nrf2-ARE pathway-related proteins in model rats, which was inhibited by Nrf2 inhibitor. Conclusions Our findings suggest that β-asarone treatment ameliorated the cerebral infarction in MCAO rats, which could be related to activation of the Nrf2-ARE pathway.

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β-asarone induces cell apoptosis, inhibits cell proliferation and decreases migration and invasion of glioma cells

Cited by 14 publications

References 21 publications

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

β‑asarone suppresses HCT116 colon cancer cell proliferation and liver metastasis in part by activating the innate immune system

Selenium-Modified Chitosan Induces HepG2 Cell Apoptosis and Differential Protein Analysis

Effects of β-Asarone on Ischemic Stroke in Middle Cerebral Artery Occlusion Rats by an Nrf2-Antioxidant Response Elements (ARE) Pathway-Dependent Mechanism

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