β-Cyclodextrin–hemin enhances tolerance against salinity in tobacco seedlings by reestablishment of ion and redox homeostasis

Zhang, Jing; Yang, Xincheng; Ren, Yanping; Yang, Bo; Liu, Ziwei; Benwu, You; Zhang, Hongxiu; Shen, Wenbiao; Chen, Xueping

doi:10.1007/s10725-016-0230-7

Cited by 7 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 ). A previous study suggested that this might be possible by upregulating the transcription of genes for enzymes related to the metabolism of the degradation products CO and GSH, which could increase the enzyme activity to help plants mitigate the oxidative damage caused by the stress ( Zhang et al, 2016 ). ZnPP could not be degraded to CO 2 in plants and reduce endogenous CO 2 production by blocking HO expression.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Hemin enhances the antioxidant defense system of rice by regulating the AsA-GSH cycle under NaCl stress

Meng,

Feng,

Zheng

et al. 2024

PeerJ

View full text Add to dashboard Cite

Abiotic stress caused by soil salinization remains a major global challenge that threatens and severely impacts crop growth, causing yield reduction worldwide. In this study, we aim to investigate the damage of salt stress on the leaf physiology of two varieties of rice (Huanghuazhan, HHZ, and Xiangliangyou900, XLY900) and the regulatory mechanism of Hemin to maintain seedling growth under the imposed stress. Rice leaves were sprayed with 5.0 μmol·L−1 Hemin or 25.0 μmol·L−1 ZnPP (Zinc protoporphyrin IX) at the three leaf and one heart stage, followed by an imposed salt stress treatment regime (50.0 mmol·L−1 sodium chloride (NaCl)). The findings revealed that NaCl stress increased antioxidant enzymes activities and decreased the content of nonenzymatic antioxidants such as ascorbate (AsA) and glutathione (GSH). Furthermore, the content of osmoregulatory substances like soluble proteins and proline was raised. Moreover, salt stress increased reactive oxygen species (ROS) content in the leaves of the two varieties. However, spraying with Hemin increased the activities of antioxidants such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) and accelerated AsA-GSH cycling to remove excess ROS. In summary, Hemin reduced the effect of salt stress on the physiological characteristics of rice leaves due to improved antioxidant defense mechanisms that impeded lipid peroxidation. Thus, Hemin was demonstrated to lessen the damage caused by salt stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Exogenous Hemin enhances the antioxidant defense system of rice by regulating the AsA-GSH cycle under NaCl stress

Meng,

Feng,

Zheng

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

“…The size, ζ-potential, PDI, and turbidity of the nanoparticles (0.5 mg/mL) were measured and compared with the initial values to evaluate the stability of the nanoparticles. The particle suspensions were divided into ten groups: six groups were adjusted to the desired pH values [2, 3, 5, 7, 9, 11], and using hydrochloric acid (0.1 M) or sodium hydroxide solution (0.1 M); three groups were heated to 25, 60, and 80 °C and then cooled to room temperature; another was conducted with different concentrations of sodium chloride (NaCl, 0, 10, 50, 100, 250, and 500 mM), respectively. The mixed solutions stood overnight at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Wang et al [6] indicated that crystalline hemin and L-arginate could prepare water-soluble hemin-arginate coacervation, which could be used as a new heme iron supplement in food additives, functional foods, and pharmaceuticals. Zhang et al [7] reported that hemin could combine with β-cyclodextrin by a cyclic oligosaccharide of seven α- linked glucose units [1, 4], leading to a significant improvement in hemin solubility. Although there was some progress on the solubility improvement of heme iron, industrialization was not easy due to the complicated preparation process.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Tadpole- and Sphere-Shaped Hemin Nanoparticles for Enhanced Solubility

Yang

Xiong

et al. 2019

Nanoscale Res Lett

View full text Add to dashboard Cite

Hemin is a potent iron supplement. A major limitation of the applicability of hemin is its extremely low aqueous solubility and bioavailability. The aim of this work is to prepare hemin nanoparticles with improved solubility. Tra nsmission electron microscopic images showed that hemin nanoparticles with different initial concentrations of hemin (0.1 and 0.5 mg/mL) were tadpole-shaped (head of approximately 200 nm and tail of 100 nm) and sphere-shaped (50–100 nm), respectively. Moreover, hemin nanoparticles exhibited higher solubility than free hemin. The solubility of sphere-shaped nanoparticles was 308.2-fold higher than that of pure hemin at 25 °C. The hemin nanoparticles were stable in acidic conditions and displayed excellent thermal stability. These results suggested that hemin nanoparticles could serve as a potential iron supplement with potential applications in the food, biomedical, and photodynamic-photothermal therapy fields. Electronic supplementary material The online version of this article (10.1186/s11671-019-2880-7) contains supplementary material, which is available to authorized users.

show abstract

“…Hemin improved the SOD, POD, and APX activities of tobacco seedlings subjected to salt stress. Hemin treatment can effectively enhance the activity of antioxidant enzymes and induce ion regulation to reduce ion accumulation and alleviate the damage that salt stress causes to tobacco seedlings [ 21 ]. The plant root system is the first organ to sense cadmium stress signals, and studies suggest that hemin can promote the formation of lateral roots of maize [ 22 ] and pak choi [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Adaptations in Rapeseed: Hemin-Induced Resilience to NaCl Stress by Enhancing Growth, Photosynthesis, and Cellular Defense Ability

Lu,

Zheng,

Feng

et al. 2024

Metabolites

View full text Add to dashboard Cite

This study aimed to investigate whether presoaking with hemin (5 μmol·L−1) could alleviate NaCl stress during rapeseed seedlings’ growth and its role in the regulation of photosynthesis. In this experiment, ‘HUAYOUZA 62 (HYZ 62)’ and ‘HUAYOUZA 158R (158R)’ were used as materials for pot experiments to study the morphology, photosynthetic characteristics, antioxidant activity, and osmoregulatory factors of seedlings under different salt concentrations, as well as the regulatory effects of hemin-presoaked seeds. Our findings revealed that, compared the control, NaCl stress inhibited the growth of two rapeseed varieties, decreased the seedling emergence rate, and increased the content of malondialdehyde (MDA), the electrolyte leakage rate (EL) and antioxidant enzyme activity. Hemin soaking alleviated the adverse effects of salt stress and increased plant height, root elongation and dry matter accumulation. Compared with all NaCl treatments, hemin significantly enhanced photosynthetic indexes, including a percent increase of 12.99–24.36% and 5.39–16.52% in net photosynthetic rate (Pn), 17.86–48.08% and 8.6–23.44% in stomatal conductivity (Gs), and 15.42–37.94% and 11.09–19.08% in transpiration rate (Tr) for HYZ62 and 158R, respectively. Moreover, hemin soaking also increased antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), reducing the malondialdehyde, and thus resulting in the alleviation of oxidative damage caused by NaCl stress. Furthermore, hemin stimulated the formation of soluble protein, which effectively regulated the osmo-protective qualities. The current findings strongly elucidate that hemin soaking could effectively alleviate the negative impacts of NaCl stress by regulating the morphological, photosynthetic, and antioxidant traits. This study provides a new idea regarding the effect of Hemin on the salt tolerance of rapeseed, and provides a basis for the practical application of Hemin in saline–alkali soil to improve the salt tolerance of cultivated rapeseed.

show abstract

β-Cyclodextrin–hemin enhances tolerance against salinity in tobacco seedlings by reestablishment of ion and redox homeostasis

Cited by 7 publications

References 45 publications

Exogenous Hemin enhances the antioxidant defense system of rice by regulating the AsA-GSH cycle under NaCl stress

Exogenous Hemin enhances the antioxidant defense system of rice by regulating the AsA-GSH cycle under NaCl stress

Preparation and Characterization of Tadpole- and Sphere-Shaped Hemin Nanoparticles for Enhanced Solubility

Metabolic Adaptations in Rapeseed: Hemin-Induced Resilience to NaCl Stress by Enhancing Growth, Photosynthesis, and Cellular Defense Ability

Contact Info

Product

Resources

About