β-Galactoside binding lectin from caddisfly larvae, Stenopsyche kodaikanalensis with selective modes of antibacterial activity: Purification and characterization

Bhuvaragavan, Sreeramulu; Arumugam, Ganesh; Paulchamy, Ramaraj; Hilda, Karuppiah; Janarthanan, Sundaram

doi:10.1016/j.ijbiomac.2018.04.158

Cited by 10 publications

(6 citation statements)

References 80 publications

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“…In addition, lectins can induce the formation of pores in the cell wall and cell membrane, leading to leakage of cytoplasmic content (Proc opio et al 2017a). The water-soluble Moringa oleifera lectin caused a reduction in the number of cells and malformations in S. marcescens (Moura et al 2017b) and the lectin from the insect Stenopsyche kodaikanalensis caused agglutination and shrinkage associated with rupture of the cell membrane in Bacillus flexus (Sreeramulu et al 2018).…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes

Silva

et al. 2021

J Appl Microbiol

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Aims: To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined. Methods and Results: PgTeL showed bacteriostatic and bactericidal effects on the strains L. monocytogenes N53-1 and EGD-e, causing morphometric alterations, cell aggregation, strong deformation and cell disruption. PgTeL inhibited biofilm formation by EGD-e and N53-1 and also interfered with the adhesion and invasion processes of EGD-e and N53-1 in HeLa cells. Finally, the chitinase activity of L. monocytogenes EGD-e was reduced in the presence of PgTeL, which can be involved in the inhibition of adhesion process. Conclusion:PgTeL is an antibacterial agent against L. monocytogenes, inhibiting growth and promoting cell death, as well as impairing biofilm formation and bacterial adhesion and invasion into human cells. Significance and Impact of the Study: The results stimulate future investigations on the potential of PgTeL for protection of contamination in food products.

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Section: Discussionmentioning

confidence: 99%

“…2017b) and the lectin from the insect Stenopsyche kodaikanalensis caused agglutination and shrinkage associated with rupture of the cell membrane in Bacillus flexus (Sreeramulu et al . 2018).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes

Silva

et al. 2021

J Appl Microbiol

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“…) and Stenopsyche kodaikanalensis (Sreeramulu et al . ) by showing the ability to agglutinate either Gram‐negative and Gram‐positive bacteria, or both.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Arumugam

Hilda

Bhuvaragavan

et al. 2019

Entomological Science

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Insects depend on lectins for non‐self recognition and clearance of invading pathogens. Naturally occurring lectin showing specificity for galactose was purified from the serum of lepidopteran pest Parasa lepida by affinity chromatography using Sepharose 6B coupled with galactose as a gel matrix. Preliminary studies on crude serum agglutinin revealed that the agglutinin molecule showed varying degrees of specificity to avian and mammalian red blood cells tested. Among them, the highest titer of 128 was recorded against rabbit red blood cell type. The agglutinin molecule in the crude serum was stable up to 60°C and at pH between 6 and 9. Also, the hemagglutinating activity was neither dependent on divalent cations nor sensitive to ethylenediaminetetraacetic acid treatment. Galactose inhibited the hemagglutinating activity at minimum inhibitory concentration of 12.5 mM and hence it was used as a ligand for affinity chromatography. Native polyacrylamide gel electrophoresis analysis revealed a single band and the molecular weight of the lectin was found to be approximately 90 kDa. Bacterial agglutination activity of the purified lectin with two significant toxin bacteria, namely Salmonella typhi and Bacillus thuringiensis, was observed.

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“…Considerando a presença de ambas as classes de moléculas no contexto infeccioso e de inflamação (Farnworth et al 2008; Cao e Guo 2016), sugerimos que galectinas possam atuar junto a esses peptídeos na promoção eliminação de patógenos específicos, trazendo maior complexidade às propriedades antibacterianas de galectinas e especificidade ao repertório de elementos microbicidas da imunidade inata. Se isso for verdade, as galectinas poderia tanto atuar na resolução de patógenos humanos, como também para o controle de infecções em animais invertebrados que dependem quase que exclusivamente de elementos da imunidade inata (Zhang et al 2020;García-Maldonado, Cano-Sánchez, e Hernández-Santoyo 2017;Sreeramulu et al 2018). Ainda, vale notar que muitos desses organismos apresentam um grande repertório de galectinas (Takeuchi 2018), ressaltando uma importância evolutiva desta família de lectinas nesse contexto.…”

Section: Discussionunclassified

“…No entanto, o reconhecimento não se resume à presença de α-gal e a lista de bactérias reconhecidas por galectinas humanas, assim como os efeitos antibacterianos devidos a este reconhecimento, se encontra na Tabela 2. Ainda, na última década diversos trabalhos caracterizaram galectinas de animais como equinodermos (Zhang et al 2020), mexilhões (García-Maldonado, Cano-Sánchez, e Hernández-Santoyo 2017) e insetos (Sreeramulu et al 2018) com potencial de provocar a morte de patógenos, destacando a importância evolutiva dos efeitos antimicrobianos dessas lectinas na imunidade de organismos que não apresentam imunidade adaptativa (Loker et al 2004).…”

Section: Galectinas E Seu Papel No Controle De Doenças Bacterianasunclassified

Novos aspectos do efeito antibacteriano de galectinas ligantes de lipopolissacarídeo

Amstalden¹

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Ao meu orientador, Prof. Dr. Marcelo Dias Baruffi, pela orientação e por todas as discussões que tangem e extrapolam o ambiente científico. Minha gratidão por ter me aceito em seu grupo e por todos os ensinamentos. À Ana Lúcia e ao Henrique, secretários do Programa de Pós Graduação em Biociências e Biotecnologia, pelo apoio e por sempre se mostrarem prestativos em ajudar e esclarecer dúvidas.À minha família: meus pais Maria Alvina Krähenbühl e Domingos Sávio Amstalden e meus irmãos Ignácio Krähenbühl Amstalden e Maria Cecília Krähenbühl Amstalden por todo o carinho e apoio à minha carreira.

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β-Galactoside binding lectin from caddisfly larvae, Stenopsyche kodaikanalensis with selective modes of antibacterial activity: Purification and characterization

Cited by 10 publications

References 80 publications

Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes

Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Novos aspectos do efeito antibacteriano de galectinas ligantes de lipopolissacarídeo

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