β-Glucan from Lentinus edodes Inhibits Nitric Oxide and Tumor Necrosis Factor-α Production and Phosphorylation of Mitogen-activated Protein Kinases in Lipopolysaccharide-stimulated Murine RAW 264.7 Macrophages

Xu, Xiaojuan; Yasuda, Michiko; Nakamura‐Tsuruta, Sachiko; Mizuno, Masashi; Ashida, Hitoshi

doi:10.1074/jbc.m111.297887

Cited by 66 publications

(30 citation statements)

References 73 publications

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“…In vivo fungal β-glucans mostly elicit anti-inflammatory, anti-oxidant and anti-tumour effects [25]. Lentinan from shiitake can regulate MAP kinases JNK1/2 and ERK1/2 and enhance the nuclear translocation of NF-κB but without NO and TNF-α production in RAW 264.7 cells [26,27]. …”

Section: Discussionmentioning

confidence: 99%

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Crespo

Guillén²,

Pablo-Maiso

et al. 2017

Food & Nutrition Research

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ABSTRACTβ-glucans exhibited in cell walls of several pathogens as bacteria or fungi are sensed by pathogen recognition receptors such as scavenger receptors present in antigen presenting cells, i.e., macrophages. β-glucans obtained from Shiitake mushrooms were chemically characterized. A β-glucan supplemented diet was assayed for 30 days in rabbits aiming to characterize the immune response elicited in blood-derived macrophages. M1 and M2 profiles of macrophage differentiation were confirmed in rabbits by in vitro stimulation with IFN-γ and IL-4 and marker quantification of each differentiation pathway. Blood derived macrophages from rabbits administered in vivo with the β-glucan supplemented diet showed higher IL-4, IFN-γ and RAGE together with lower IL-10 relative expression, indicative of an ongoing immune response. Differences in IL-1β, IL-13 and IL-4 expression were also found in rabbit sera by ELISA suggesting further stimulation of the adaptive response. Recent challenges in the rabbit industry include the search of diet supplements able to elicit an immune stimulation with particular interest in facing pathogens such as viruses or bacteria. β–glucans from fungi may contribute to maintain an immune steady state favouring protection and thus reducing antibiotic treatment.

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Section: Discussionmentioning

confidence: 99%

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Crespo

Guillén²,

Pablo-Maiso

et al. 2017

Food & Nutrition Research

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“…The development of the inflammatory process involves the production of pro-inflammatory cytokines and inflammatory mediators, such as tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), nitric oxide (NO) and prostaglandin E2 (PGE2), which are the main factors that mediate the LPS-induced toxic effects [9][10][11]. The inhibition of the excess production of pro-inflammatory factors is required for decreasing inflammatory reactions, which are the main cause of inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

The effect of anti-inflammatory properties of ferritin light chain on lipopolysaccharide-induced inflammatory response in murine macrophages

Fan

Zhang

Cai

et al. 2014

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Ferritin light chain (FTL) reduces the free iron concentration by forming ferritin complexes with ferritin heavy chain (FTH). Thus, FTL competes with the Fenton reaction by acting as an antioxidant. In the present study, we determined that FTL influences the lipopolysaccharide (LPS)-induced inflammatory response. FTL protein expression was regulated by LPS stimulation in RAW264.7 cells. To investigate the role of FTL in LPS-activated murine macrophages, we established stable FTL-expressing cells and used shRNA to silence FTL expression in RAW264.7 cells. Overexpression of FTL significantly decreased the LPS-induced production of tumor necrosis factor alpha (TNF-α), interleukin 1β (IL-1β), nitric oxide (NO) and prostaglandin E2 (PGE2). Additionally, overexpression of FTL decreased the LPS-induced increase of the intracellular labile iron pool (LIP) and reactive oxygen species (ROS). Moreover, FTL overexpression suppressed the LPS-induced activation of MAPKs and nuclear factor-κB (NF-κB). In contrast, knockdown of FTL by shRNA showed the reverse effects. Therefore, our results indicate that FTL plays an anti-inflammatory role in response to LPS in murine macrophages and may have therapeutic potential for treating inflammatory diseases.

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“…As described in Materials and Methods, the chemical analysis has indicated that LE is chiefly made up of polysaccharides, which have been demonstrated to exhibit significant biological effects such as anti-inflammatory and anti-oxidative activity in previous reports [32,33]. In addition, several researchers have revealed that lentinan, β-glucan from Lentinus edodes, induces immunomodulatory and immunosuppressive behaviors under different conditions through MAPK signaling pathways mediated by ERK1/2 and JNK1 /2 [3,5]. Furthermore, the recent study provides evidence that lentinan could inhibit gut inflammation via modulation of NF-κB activity in the experimental models [34].…”

Section: Discussionmentioning

confidence: 99%

Lentinus edodesSuppresses Allergen-Induced Airway Hyperresponsiveness and Inflammation by Downregulating Nuclear Factor-kappa B Activity in a Murine Model of Allergic Asthma

Yan

Choi

2014

Korean J Phys Anthropol

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β-Glucan from Lentinus edodes Inhibits Nitric Oxide and Tumor Necrosis Factor-α Production and Phosphorylation of Mitogen-activated Protein Kinases in Lipopolysaccharide-stimulated Murine RAW 264.7 Macrophages

Cited by 66 publications

References 73 publications

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit

The effect of anti-inflammatory properties of ferritin light chain on lipopolysaccharide-induced inflammatory response in murine macrophages

Lentinus edodesSuppresses Allergen-Induced Airway Hyperresponsiveness and Inflammation by Downregulating Nuclear Factor-kappa B Activity in a Murine Model of Allergic Asthma

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