β-Lactoglobulin Gene Polymorphisms in Sheep and Effects on Milk Production Traits: A Review

Selvaggi, Maria

doi:10.14737/journal.aavs/2015/3.9.478.484

Cited by 23 publications

(26 citation statements)

References 22 publications

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“…The results of current study are in line with the findings of previous researchers ( Huma et al, 2018 ; Ozrenk and Inci, 2008 ; Strzałkowska et al, 2014 ). Sheep milk is rich in casein and whey proteins 4.2–5.2 g/100 g and 1.02–1.3 g/100 g respectively ( Barros et al., 2017 ; Selvaggi et al, 2015 ; Selvaggi et al, 2017 ) and protein content in camel milk was between 2.15%–4.90% ( Al Kanhal and AL haj, 2010 ). The current results of casein and whey proteins in camel milk are also similar with the findings of other researchers ( Babiker and El-Zubeir, 2014 ; El-Agamy et al, 2009 ; Khaskheli et al, 2005 ; Musaad et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

Yasmin

Iqbal

Liaqat

et al. 2020

Food Sci Anim Resour

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The aim of study was to scrutinize the physicochemical and protein profile of milk obtained from local Pakistani breeds of milch animals such as Nilli-Ravi buffalo, Sahiwal cow, Kajli sheep, Beetal goat and Brela camel. Physicochemical analysis unveiled maximum number of total solids and protein found in sheep and minimum in camel. Buffalo milk contains the highest level of fat (7.45%) while camel milk contains minimum (1.94%). Ash was found maximum in buffalo (0.81%) and sheep (0.80%) while minimum in cow’s milk (0.71%). Casein and whey proteins were separated by subjecting milk to isoelectric pH and then analyzed through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed heterogeneity among these species. Different fractions including α S1 , α S2 , κ-casein, β-casein and β-lactoglobulen (β-Lg) were identified and quantitatively compared in all milk samples. Additionally, this electrophoretic method after examining the number and strength of different protein bands (α S1 , α S2 , β-CN, α-LAC, BSA, and β-Lg, etc.), was helpful to understand the properties of milk for different processing purposes and could be successfully applied in dairy industry. Results revealed that camel milk was best suitable for producing allergen free milk protein products. Furthermore, based on the variability of milk proteins, it is suggested to clarify the phylogenetic relationships between different cattle breeds and to gather the necessary data to preserve the genetic fund and biodiversity of the local breeds. Thus, the study of milk protein from different breed and species has a wide range of scope in producing diverse protein based dairy products like cheese.

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Section: Resultsmentioning

confidence: 99%

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

Yasmin

Iqbal

Liaqat

et al. 2020

Food Sci Anim Resour

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“…The β-LG gene polymorphism can be used as a molecular marker for milk yield and milk composition traits (Selvaggi et al, 2015). The β-LG genotype in this study was not significant differences on milk protein percentage in Saanen goat.…”

Section: Resultsmentioning

confidence: 44%

“…The results of these studies are possible affected by several factors, some of them were population size, type of livestock, frequency of genotypes, and statistical analysis models. This different of result may be to breed difference leading to different structure of β-LG gene and the frequency of genotypes, number of population and difference in statistical analysis models used (Selvaggi et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Some of the previous studies indicate that the β-LG genotype (AA and BB) is associated with milk proteinls, while in this study showed showed that β-LG genotype (AA, AG, GG) was not associated with milk protein. Not all of the results from different studies can be compared with each other for several reasons, including differences in livestock breeding types and genotype frequencies (Selvaggi et al, 2015). Furthermore Selvaggi et al (2015) stated that the results of research on the association of the β-LG gene with different milk production traits can be caused by a lack of relationship information in the livestock of sample population (differences in the background of the origin of the livestock population).…”

Section: Resultsmentioning

confidence: 99%

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Polymorphism of β-lactoglobulin (β-LG)|SacII gene and its association to milk protein and milk production in Saanen goats

Ambarwati

Sutopo

Kurnianto

2019

J. Indonesian Trop. Anim. Agric.

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The objectives of this study were to analyze and identify the β-LG genotype and examine its association with milk protein and milk production of Saanen goats. Materials used in this study were DNA that originated from 22 Saanen does and its milk protein and milk production recording that was taken at 2,5; 3,0; 3,5 and 4,0 years old of Saanen does. DNA was analyzed using PCR-RFLP method with SacII restriction enzyme to identify genotype of B-LG gene and then were sequenced for each genotype. Sequencing data was analyzed using Clustal W in MEGA7.0, while association of B-LG gene genotype with milk protein and milk production was analyzed using General Linear Model (GLM) in SAS 9.0. The results showed that two alleles (A and G), forming two homozygous genotypes (AA and GG) and one heterozygous genotype (AG) were found, but there were no significant different within genotype on milk protein and milk production. In conclusion, there was no association between β-LG genotype and milk protein and milk production of Saanen goats.

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“…Based on published studies of ruminants [16,17,41], we have assumed that equine LGB gene variants can serve as valuable markers of equine milk composition, including LGB content and gross milk composition traits. The most repeatable association recorded in our study was the relationship between particular SNPs (such as LGB1 rs1143515669, rs1144647991 and rs1140322039) and basic milk composition traits (total milk protein or total milk fat concentration).…”

Section: Discussionmentioning

confidence: 99%

Genes encoding equine β-lactoglobulin (LGB1 and LGB2): Polymorphism, expression, and impact on milk composition

et al. 2020

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β-lactoglobulin is one of the most abundant milk whey proteins in many mammal species, including the domestic horse. The aim of this study was to screen for polymorphism in the equine LGB1 and LGB2 gene sequences (all exons, introns, and 5'-flanking region) and to assess potential relationship of particular genotypes with gene expression levels (measured in milk somatic cells) and milk composition traits (protein, fat, lactose, and total β-lactoglobulin content). Direct DNA sequencing analysis was performed for twelve horse breeds: Polish Primitive Horse (PPH), Polish Coldblood Horse (PCH), Polish Warmblood Horse (PWH), Silesian, Hucul, Fjording, Haflinger, Shetland Pony, Welsh Pony, Arabian, Thoroughbred, and Percheron-and revealed the presence of 83 polymorphic sites (47 and 36 for LGB1 and LGB2 genes, respectively), including eight that were previously unknown. Association analysis of the selected polymorphisms, gene expression, and milk composition traits (conducted for the PPH, PCH, and PWH breeds) showed several statistically significant relationships; for example, the two linked LGB1 SNPs (rs1143515669 and rs1144647991) were associated with total milk protein content (p < 0.01). Our study also confirmed that horse breed had significant impact on both gene transcript levels (p < 0.01) and on milk LGB content (p < 0.05), whereas an influence of lactation period was seen only for gene relative mRNA abundances (p < 0.01).

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β-Lactoglobulin Gene Polymorphisms in Sheep and Effects on Milk Production Traits: A Review

Cited by 23 publications

References 22 publications

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

Polymorphism of β-lactoglobulin (β-LG)|SacII gene and its association to milk protein and milk production in Saanen goats

Genes encoding equine β-lactoglobulin (LGB1 and LGB2): Polymorphism, expression, and impact on milk composition

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