β-PVDF Membranes Induce Cellular Proliferation and Differentiation in Static and Dynamic Conditions

Abstract

Bone marrow cells are a potential source to induce different lineage cells which can be used to rebuild or replace damaged tissues using a Tissue Engineering (TE) approach. However, TE strategies usually require the use of a material to support the development of a biological tissue. Beta-polyvinylidene fluoride (β-PVDF) is a biocompatible, thermoplastic with piezo-electrical properties that has been shown to provide a good cellular attachment and therefore might present advantageous properties as a scaffold material for cell seeding/culturing. The present study describes the characterization of β-PVDF membranes as a support material for growth and differentiation of goat marrow cells (GMCs) into osteoblasts, leading to the formation of substitutes for tissue regeneration. The obtained results suggest that β-PVDF piezoelectric properties influence cellular behavior. β- PVDF membranes not only enhance GMCs adherence and proliferation but also improve differentiation towards the osteogenic phenotype both in static and dynamic culture conditions. Furthermore, β-PVDF membranes exhibit very promising properties, suggesting that this material provides adequate support for the seeding and the development of undifferentiated cells towards a desired phenotype.

Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates

“…Among these polymers, poly(vinylidene fluoride) (PVDF) is the one with the largest piezo-, pyro- and ferroelectric responses [ 15 ]. Also, this biocompatible polymer shows good osteoinductive results, not only on dynamic, but also on static cultures, proving that it is suitable for stem cell culture and differentiation into the osteogenic lineage [ 16 , 17 , 18 , 19 , 20 ].…”

Modulation of biocompatibility on poly(vinylidene fluoride) and polysulfone by oxygen plasma treatment and dopamine coating

“…Poly(vinylidene fluoride) (PVDF) and polysulfone (PSf) are two thermoplastic polymers with excellent mechanical strength, good durability, and nontoxicity. For example, PVDF was used for the applications in tissue engineering,1 surgical mesh,2, 3 and synthetic blood vessels,4, 5 and nerve regeneration 6–8. PSf carbon fiber was used as implant materials beside its common applications for filtration membrane,9, 10 pervaporation,11–13 gas separation,14, 15 hemodialysis,16–19 and composite fuel cell membrane 20–22.…”

Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

“…The possibility of tailoring PVDF properties and microstructure, allows new and challenging applications in the biomedical area, not only in device applications but also induce targeted cell responses [6]. Previous studies investigated the biocompatibility of PVDF films and showed the PVDF as a very promising material for biomedical applications [6,7]. In this sense, the effect of the phase of PVDF and its polarization state has been previously studied [6].…”