β-Sitosterol, a phytocompound from
 Parthenium hysterophorus,
 reveals anti-diabetic properties through α-Amylase inhibition: an i
 n-silico
 and
 in-vitro
 analysis

Ravi, Lokesh; Girish, Shabari; Bk, Anirudh Sreenivas; R, Shree Kumari G; Archana, O; Manjunathan, Reji

doi:10.1080/07391102.2023.2186703

Cited by 6 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, in silico investigations revealed a large number of molecular interactions with key α-amylase amino acid residues. Moreover, β-Sitosterol ( Figure 9 f) was identified as an effective α-amylase inhibitor from Parthenium hysterophorus leaf extract [ 163 ]. According to the molecular dynamics analyses, β-sitosterol has a larger binding energy than acarbose and the highest stability with α-amylase enzymes.…”

Section: Secondary Metabolites Isolated From Various Plant Sources As...mentioning

confidence: 99%

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

Kashtoh

Baek

2023

Plants

View full text Add to dashboard Cite

The rising predominance of type 2 diabetes, combined with the poor medical effects seen with commercially available anti-diabetic medications, has motivated the development of innovative treatment approaches for regulating postprandial glucose levels. Natural carbohydrate digestion enzyme inhibitors might be a viable option for blocking dietary carbohydrate absorption with fewer side effects than manufactured medicines. Alpha-amylase is a metalloenzyme that facilitates digestion by breaking down polysaccharides into smaller molecules such as maltose and maltotriose. It also contributes to elevated blood glucose levels and postprandial hyperglycemia. As a result, scientists are being urged to target α-amylase and create inhibitors that can slow down the release of glucose from carbohydrate chains and prolong its absorption, thereby resulting in lower postprandial plasma glucose levels. Natural α-amylase inhibitors derived from plants have gained popularity as safe and cost-effective alternatives. The bioactive components responsible for the inhibitory actions of various plant extracts have been identified through phytochemical research, paving the way for further development and application. The majority of the findings, however, are based on in vitro investigations. Only a few animal experiments and very few human investigations have confirmed these findings. Despite some promising results, additional investigation is needed to develop feasible anti-diabetic drugs based on plant-derived pancreatic α-amylase inhibitors. This review summarizes the most recent findings from research on plant-derived pancreatic α-amylase inhibitors, including plant extracts and plant-derived bioactive compounds. Furthermore, it offers insights into the structural aspects of the crucial therapeutic target, α-amylases, in addition to their interactions with inhibitors.

show abstract

Section: Secondary Metabolites Isolated From Various Plant Sources As...mentioning

confidence: 99%

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

Kashtoh

Baek

2023

Plants

View full text Add to dashboard Cite

show abstract

“…The presence of ɣ-sitosterol as a major compound in hexane extract could be implicated in the cholinesterase, butyrylcholinesterase, and amylase inhibition activity as a recent study revealed that β-sitosterol was effective against Alzheimer's [68] and diabetes. [69] Furthermore, it was found that linoleic acid, which was accumulated in the hexane extract, contributed to the enhancement of digestive enzyme ability. [70] 2 previous study on the antiproliferative activity of Thymus species was mainly associated with the composition of their essential oils.…”

Section: Enzyme Inhibitionmentioning

confidence: 99%

LC‐MS/MS and GC‐MS profiling, antioxidant, enzyme inhibition, and antiproliferative activities of Thymus leucostomus Hausskn. & Velen. extracts

Fayez,

Fahmy,

Zengin

et al. 2023

Archiv der Pharmazie

View full text Add to dashboard Cite

The chemical composition as well as antioxidant, antiproliferative, and enzyme inhibition activities of extracts from aerial parts of Thymus leucostomus H ausskn. & V elen. obtained with hexane, methanol, and water were evaluated. Results showed that the methanol extract had significantly (p < 0.05) the highest total phenolic content (TPC; 107.80 mg GAE/g) and total flavonoids content (TFC; 25.21 mg RE/g) followed by the aqueous extract (102.72 mg GAE/g and 20.88 mg RE/g, respectively). LC‐MS/MS‐guided profiling of the three extracts revealed that rosmarinic acid (34.8%), hesperetin (42.9%), and linoleic acid (18%) were the dominant compounds in the methanol, aqueous and hexane extracts, respectively. GC‐MS analysis of the hexane extract showed that ɣ‐sitosterol (29.9%) was the major constituent. The methanol extract displayed significantly (p < 0.05) the highest Cu++, Fe+++, and Mo(VI) ions scavenging and reducing properties while the aqueous extract exerted significantly (p < 0.05) the highest metal chelating power (42.51 mg EDTAE/g). Both the hexane and methanol extracts effectively inhibited the acetylcholinesterase enzyme (2.63 and 2.65 mg GALAE/g, respectively) while the former extract exerted significantly (p < 0.05) the highest butyrylcholinesterase (2.32 mg GALAE/g), tyrosinase (19.73 mg KAE/g), and amylase (1.16 mmol ACAE/g) inhibition capacity. The aqueous extract exhibited the best glucosidase inhibition property (0.49 mmol ACAE/g). The methanol and hexane extracts exerted a higher cytotoxic effect on HT‐29 (IC50: 8.12 µg/mL) and HeLa (IC50 = 8.08 µg/mL) cells, respectively. In conclusion, these results provide valuable insight into the potential use of T. leucostomus bioactive extracts in different pharmaceutical applications.

show abstract

Phytomedicine approach for management of diabetes mellitus: an overview of scientifically confirmed medicinal plants with hypoglycaemic properties and their probable mechanism of action

Okoduwa,

Mhya,

Abdulwaliyu

et al. 2024

Phytochem Rev

View full text Add to dashboard Cite

β-Sitosterol, a phytocompound from Parthenium hysterophorus, reveals anti-diabetic properties through α-Amylase inhibition: an i n-silico and in-vitro analysis

Cited by 6 publications

References 25 publications

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

New Insights into the Latest Advancement in α-Amylase Inhibitors of Plant Origin with Anti-Diabetic Effects

LC‐MS/MS and GC‐MS profiling, antioxidant, enzyme inhibition, and antiproliferative activities of Thymus leucostomus Hausskn. & Velen. extracts

Phytomedicine approach for management of diabetes mellitus: an overview of scientifically confirmed medicinal plants with hypoglycaemic properties and their probable mechanism of action

Contact Info

Product

Resources

About