β-Tricalcium phosphate promotes cell proliferation, osteogenesis and bone regeneration in intrabony defects in dogs

Neamat, Amany; Gawish, Ahmed; Gamal‐Eldeen, Amira M.

doi:10.1016/j.archoralbio.2009.09.003

Cited by 33 publications

(20 citation statements)

References 21 publications

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“…β-TCP 3D scaffolds could promote ostognenic differentiation of human adipose stem cells in vitro without addition of ostogenic differentiation media [15] . In line with previous study [8,16,17] , in present work after co-cultured with β-TCP for 3 days, there was a signifi cant induction of ossifi cation of osteoblast without adding osteogenic media. Furthermore, [Ca 2+ ] i increased significantly after co-cultured with β-TCP.…”

Section: Resultssupporting

confidence: 93%

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

Zhang

Dai

et al. 2011

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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β-TCP, as one of calcium phosphates ceramics, exerts perfect biocompatibility and osteoconductivity, and is clinically used as a bone graft substitute for decades. Consequently, the effects of β-TCP ceramics on intracellular Ca 2+ concentration, mineralization of osteoblast and BSA protein structure were studied. Results showed that β-TCP could increase the intracelluar Ca 2+ concentration and mineralization of osteoblast, indicating that β-TCP ceramics could take part in the organic metabolism and the degradation product had no detrimental effect on osteoblast in vitro. Furthermore, β-TCP ceramics could increase the content of α-helix and β-pleated sheet and change BSA into more ordering structure, those changes might be favorable for the biomineralization after β-TCP ceramics implanted.

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Section: Resultssupporting

confidence: 93%

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

Zhang

Dai

et al. 2011

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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“…Moreover, there was no statistical difference between the groups regarding the amount of alumina used (P > 0.05). These results indicate that different amounts of alumina within β-TCP did not affect the cell viability, which was already expected as β-TCP and Al 2 O 3 have been investigated and exhibited high biocompatibility [51][52][53] . Genotoxicity was evaluated in this study through the micronucleus test.…”

Section: Resultssupporting

confidence: 69%

Preparation, Characterization and Biological Studies of Β-TCP and Β-TCP/Al2O3 Scaffolds Obtained by Gel-Casting of Foams

et al. 2017

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Replacement tissues for tissue engineering can be produced by seeding human cells onto scaffolds. In order to guarantee adequate bio-compatibility, porosity and mechanical resistance for promoting cellular growth, proliferation and differentiation within scaffold structures, it is necessary to investigate and improve materials and processing routes. β-tricalcium phosphate can be considered a very suitable bio-ceramic material for bone therapy because of its biocompatibility, osteo-conductivity and neovascularization potential. Alumina is commonly used as a sintering additive. In this study, β-TCP and β-TCP/Al 2 O 3 scaffolds were obtained by gel-casting method. The scaffolds showed high porosity (86-88%) and pore sizes ranging from 200 to 500 μm. Even though alumina did not promote improvement in β-TCP/Al 2 O 3 scaffolds in terms of mechanical performance, they showed great cytocompatibility as there was no cytotoxic and genotoxic effect. Therefore, β-TCP and β-TCP/Al 2 O 3 scaffolds are good candidates for application in tissue engineering.

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“…Not only should the material be porous to serve as a scaffold for capillary growth, the material should have excellent biocompatibility, osteoconductivity, and a complete lack of antigenicity [7]. Ideally, a scaffold will offer mechanical support and will be resorbed as new bone and tissue growth occur.…”

Section: Introductionmentioning

confidence: 99%

Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds

2012

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Objectives To evaluate the effects of SiO2 (0.5 wt %) and ZnO (0.25 wt %) dopants on the mechanical and biological properties of tricalcium phosphate (TCP) scaffolds with three dimensionally (3D) interconnected pores. Methods Scaffolds were created with a commercial 3D printer. Post sintering phase analysis was determined by x-ray diffraction. Surface morphology of the scaffolds was examined by field emission electron microscopy. Mechanical strength was evaluated with a screw driven universal testing machine. MTT assay was used for cellular proliferation characteristics and cellular morphology was examined by field emission electron microscopy. Results Addition of dopants into TCP increased the average density of pure TCP from 90.8 ± 0.8% to 94.1 ± 1.6% and retarded the β to α phase transformation at high sintering temperatures, which resulted in up to 2.5 fold increase in compressive strength. In vitro cell-materials interaction studies, carried out using hFOB cells, confirmed that the addition of SiO2 and ZnO to the scaffolds facilitates faster cell proliferation when compared to pure TCP scaffolds. Significance Addition of SiO2 and ZnO dopants to the TCP scaffolds showed increased mechanical strength as well as increased cellular proliferation.

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β-Tricalcium phosphate promotes cell proliferation, osteogenesis and bone regeneration in intrabony defects in dogs

Cited by 33 publications

References 21 publications

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

Effects of β-TCP ceramics on intracellular Ca2+ concentration, mineralization of osteoblast and protein structure

Preparation, Characterization and Biological Studies of Β-TCP and Β-TCP/Al2O3 Scaffolds Obtained by Gel-Casting of Foams

Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds

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