βIII Spectrin Regulates the Structural Integrity and the Secretory Protein Transport of the Golgi Complex

Salcedo-Sicilia, Laia; Granell, Susana; Jović, Marko; Sicart, Adrià; Mato, Eugènia; Johannes, Ludger; Balla, Tamás; Egea, Gustavo

doi:10.1074/jbc.m112.406462

Cited by 19 publications

(22 citation statements)

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“…Cofilin is associated with the TGN, and plays a role in cargo sorting[118, 119]. There is also suggestion of a spectrin-actin meshwork at or close to the Golgi, with its integrity affecting Golgi function[108, 120, 121]. Finally, multiple myosins (in the I, II, VI and XVIII families) play roles in Golgi structure/function[113, 117, 122].…”

Section: Golgimentioning

confidence: 99%

“…Two issues should be raised here. First, these proteins often “enrich” at Golgi, but are also abundant in vesicular structures surrounding Golgi or elsewhere[115, 121]. Sometimes, the proteins shown to affect Golgi structure have little or no actual enrichment there[110, 111].…”

Section: Golgimentioning

confidence: 99%

“…Suppression of βIII spectrin disrupts Golgi structure[121]. However, the localization pattern of βIII spectrin is not confined to Golgi, and appears rich on vesicular structures elsewhere in the cytoplasm[121, 133]. This feature might suggest a role for spectrin more in transport to/from Golgi rather than directly in Golgi morphology.…”

Section: Golgimentioning

confidence: 99%

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Connecting the Cytoskeleton to the Endoplasmic Reticulum and Golgi

2014

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A tendency in cell biology is to divide and conquer. For example, decades of painstaking work have led to an understanding of endoplasmic reticulum (ER) and Golgi structure, dynamics, and transport. In parallel, cytoskeletal researchers have revealed a fantastic diversity of structure and cellular function in both actin and microtubules. Increasingly, these areas overlap, necessitating an understanding of both organelle and cytoskeletal biology. This review addressesconnections between the actin/microtubule cytoskeletons and organelles in animal cells, focusing on threetopics: ER structure/function, ER-to-Golgi transport; and Golgi structure/function. Making these connections has been challenging, due to 1) the small sizes and dynamic characteristics of some components, 2) the fact that organelle-specific cytoskeleton can easily be obscured by more abundant cytoskeletal structures, and 3) the difficulties in imaging membranes and cytoskeleton simultaneously, especially at the ultra-structural level. One major concept is that the cytoskeleton is frequently used to generate force for membrane movement, with two potential consequences: translocation of the organelle, or deformation of the organelle membrane. While initially discussing issues common to metazoan cells in general, we subsequently highlight specific features of neurons, since these highly polarized cells present unique challenges for organellar distribution and dynamics.

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Section: Golgimentioning

confidence: 99%

Section: Golgimentioning

confidence: 99%

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Connecting the Cytoskeleton to the Endoplasmic Reticulum and Golgi

2014

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“…Using a spectrin III subunit containing the green fluorescent protein tag in transfected COS cells, spectrin decorated the tubular-vesicular transport intermediates moving along microtubules between the endoplasmic reticulum and the Golgi apparatus, requiring the participation of the MAD1 domain [45]. Salcedo-Sicilia et al [46] suggested that III spectrin helps to maintain the structural integrity and secretory activity of the Golgi complex by associating with phosphatidylinositol 4-phosphate (PI4P).…”

Section: Spectrin and Intracellular Trafficmentioning

confidence: 99%

Spectrin: Structure, function and disease

Zhang

Zhao

et al. 2013

Sci. China Life Sci.

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Spectrin is a large, cytoskeletal, and heterodimeric protein composed of modular structure of and subunits, it typically contains 106 contiguous amino acid sequence motifs called "spectrin repeats". Spectrin is crucial for maintaining the stability and structure of the cell membrane and the shape of a cell. Moreover, it contributes to diverse cell functions such as cell adhesion, cell spreading, and the cell cycle. Mutations of spectrin lead to various human diseases such as hereditary hemolytic anemia, type 5 spinocerebellar ataxia, cancer, as well as others. This review focuses on recent advances in determining the structure and function of spectrin as well as its role in disease.erythrocyte, spectrin, cell cycle, mass spectrometry, disease Citation:Zhang R, Zhang C Y, Zhao Q, et al. Spectrin: Structure, function and disease.

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“…It is known that at a short pulse duration accumulation of radicals and active particles in the substrate runs slower [4] and, therefore, erythrocytes also hemolyze slower in such situation, and a greater quantity of cells retain the viability. A protein cytoskeleton of erythrocytes responsible for elasticity and integrity of the membrane [12] is subjected to destructive changes to the greater extent after exposure to spark discharge plasma with 1,500 μs pulse duration and ultraviolet radiation of the mercury lamp.…”

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confidence: 99%

Analysis of Cytotoxic Effects of Medical Gas-Discharge Devices

Astafyeva¹,

Иванова²

2017

Sovrem Tehnol Med

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Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian FederationThe aim of the investigation was to analyze cytotoxic effects of various gas-discharge technologies. Materials and Methods. The following gas-discharge devices were used in the work: Pilimin series IR-1, IR-10 and Brig -radiation of spark discharge plasma; DBK-9 low-pressure mercury lamp -ultraviolet radiation; Corona device for darsonvalization -silent electric corona discharge. The objects of investigation were erythrocytes of Wistar rats, and lymphoid cells of Pliss lymphosarcoma.Results. All examined gas-discharge devices were established to possess membranotoxic and cytotoxic effects. The total number of erythrocytes and lymphoid cells diminished, while the number of nonviable cells grew with the increase of exposure time. Erythrocyte membrane was least resistant to the radiation of the spark discharge plasma with 1,500 μs pulse duration and to ultraviolet radiation of the mercury lamp, whereas erythrocytes were less resistant to the radiation by the gas-discharge devices than lymphoid cells. Pliss lymphosarcoma cells appeared to be more sensitive to the action of spark discharge plasma and corona discharge with 1-10 μs pulse duration. Radiation of spark discharge plasma with 150 μs pulse duration exhibited equally membranotoxic and cytotoxic effect.Conclusion. The results of assessing cytotoxic effect of various gas-discharge devices help to determine the direction of investigations when mechanisms of action of gas-discharge technologies are explored, and to recommend, in particular, a more profound study of such parameter as pulse discharge duration.Key words: gas-discharge technologies; radiation of spark discharge plasma; ultraviolet radiation; Darsonval corona discharge.For contacts: Irina P. Ivanova, e-mail: ivanova.ip@mail.ru Analysis of Cytotoxic Effect of Medical Gas-Discharge DevicesThe increasing number of neoplastic and infectious processes resistant to traditional methods of treatment (radiotherapy, chemotherapy, antibiotic therapy) stipulate the search and development of novel effective methods of inhibiting proliferative activity of neoplastic and prokaryotic cells, which possess cytotoxic effect and do not cause pronounced toxic effect on the organism as a whole. In this connection, gas-discharge plasma technologies applied both in Russia and abroad are of great interest [1][2][3]. Such medical gas-discharge devices as quartz lamp (discharge of mercury vapors) [4] and apparatus for darsonvalization (silent corona discharge) [5] are widely being used in Russia. Biological effects of recently developed gas-discharge Brig devices (Sarov, Nizhny Novgorod region, Russia) and Pilimin (Moscow, Russia), based on radiation of plasma spark discharge, are being investigated [6][7][8][9].To assess the efficacy of cytotoxic effect of any physical or chemical factor, it is necessary to carry out a primary screening to evaluate the resistance of the membrane and the cell as a whole to the acti...

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βIII Spectrin Regulates the Structural Integrity and the Secretory Protein Transport of the Golgi Complex

Cited by 19 publications

References 50 publications

Connecting the Cytoskeleton to the Endoplasmic Reticulum and Golgi

Connecting the Cytoskeleton to the Endoplasmic Reticulum and Golgi

Spectrin: Structure, function and disease

Analysis of Cytotoxic Effects of Medical Gas-Discharge Devices

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