ΔSCOPE: A new method to quantify 3D biological structures and identify differences in zebrafish forebrain development

Schwartz, Morgan; Schnabl, Jake; Litz, Mackenzie P.H.; Baumer, Benjamin S.; Barresi, Michael

doi:10.1016/j.ydbio.2019.11.014

Cited by 3 publications

(23 citation statements)

References 40 publications

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“…Although, little documentation currently exists to support the existence of astroglial subtypes in zebrafish, the no-isthmus (noi; pax2a) mutants have a loss of reticulate astrocytes in the glia limitans at the optic nerve, which results in pathfinding defects and impaired fasciculation by axons of the optic chiasm and POC (Macdonald et al, 1997). As alluded to previously, Gfap+ astroglial fibers develop into condensed midline bridges in the diencephalon and telencephalon, and directly interact with commissural and retinal axons during and after midline crossing (Barresi et al, 2005;Schwartz et al, 2020).…”

Section: Introductionmentioning

confidence: 61%

“…We have previously shown that the forebrain commissures develop in direct contact with astroglial cells expressing Gfap (Figure 1 A, green; (Barresi et al, 2005;Schwartz et al, 2020)). At 48 hpf, Gfap densely populated the most superficial/basal regions of the forebrain and specifically overlapped with and interlaced through each commissure (Figure 1 A-E, brackets; Sup.…”

Section: Defining the Differentiated Forebrainmentioning

confidence: 88%

“…• C and staged in hours post-fertilization (hpf) (Kimmel, Ballard, et al, 1995). Immunoctyochemistry was carried out as described previously (Johnson et al 2014;Johnson et al, 2016;Schwartz et al, 2020). Briefly, embryos were fixed with 4% paraformaldehyde with 10% dimethylsulfoxide (DMSO) for 3 hrs at room temp or 1 hour at room temp followed by 16 hours at 4…”

Section: Whole Mount Immunocytochemistrymentioning

confidence: 99%

“…AT and Zrf1-4 channels were then processed using ilastik to improve the signal to noise ratios and generate signal probability values for each pixel. Output ilastik files for Zrf1-4 experimental groups were finally processed using the ∆ Scope workflow identical to (Schwartz et al, 2020).…”

Section: Scope Data Processingmentioning

confidence: 99%

“…Prior to commissure formation, processes of these Gfap+ cells appear to ubiquitously cover the basal or superficial most extents of the forebrain, however Gfap labeling soon condenses into midline spanning swaths of astroglia corresponding to the presumptive diencephalic and telencephalic commissural regions (Barresi et al, 2005). Moreover, axonal growth is quantitatively correlated with the movement of these astroglial cells over the course of commissure formation, strongly suggesting that these "glial bridges" provide a supportive substrate for pathfinding commissural axons (Barresi et al, 2005;Schwartz et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl

Litz

Schneider

et al. 2020

Preprint

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During embryonic development of bilateral organisms, neurons send axons across the midline at specific points to connect the two halves of the nervous system with a commissure. Little is known about the cells at the midline that facilitate this tightly regulated process. We exploit the con served process of vertebrate embryonic development in the zebrafish model system to elucidate the identity of cells at the midline that may facilitate postoptic (POC) and anterior commissure (AC) development. We have discovered that three differentgfap+ astroglial cell morphologies persist in contact with pathfinding axons throughout commissure formation. Similarly, olig2+ progenitor cells occupy delineated portions of the postoptic and anterior commissures. These early olig2+ progenitors demonstrate glial-like morphologies despite the lack of a myelination marker. Moreover, we conclude that both the gfap+ and olig2+progenitor cells give rise to neuronal populations in both the telencephalon and diencephalon. Interestingly, these varied cell populations showed significant developmental heterochrony between the telencephalon and diencephalon. Lastly, we also showed that fli1a+ mesenchymal cells migrate along the presumptive commissure regions before and during midline axon crossing. Furthermore, following commissure maturation, specific blood vessels formed at the midline of the POC and immediately ventral and parallel to the AC. This comprehensive account of the cellular populations that correlate with the timing and position of commissural axon pathfinding has supported the conceptual modeling and identification of the early forebrain architecture that may be necessary for proper commissure development.

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Section: Introductionmentioning

confidence: 61%

Section: Defining the Differentiated Forebrainmentioning

confidence: 88%

Section: Whole Mount Immunocytochemistrymentioning

confidence: 99%

Section: Scope Data Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl

Litz

Schneider

et al. 2020

Preprint

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Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl

Litz

Schneider

et al. 2021

Developmental Neurobiology

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Development of the central nervous system (CNS) is a complex process that requires the coordinated generation of the correct number, position, and differentiation of neuronal and glial lineages. Vertebrate brain development involves the creation of a diverse array of cell types, the formation of complex neural circuits, and cell migration with tissue level morphogenesis (Kuzmicz-Kowalska & Kicheva, 2020;Lindsey et al., 2018;Namba & Huttner, 2017). The zebrafish, Danio rerio, enables direct and transparent access to the early embryo and possesses growing stockpiles of genetic tools to

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Machine Perception of Political Manifestos in Predicting Performance of Public Office Holders

Olajide Ajayi,

Akinrolabu

2023

JPPUMA

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Electorates expect politicians seeking public office to make known in advance, their intended programs in form of proposal. This is usually presented in speech in form of manifesto. Times within number, manifestos have always precede voting proper whereby the electorates evaluate politicians based on their manifestos. While intention is socially difficult to measure, this study adopts artificial neural network machine learning approach to map-measure the manifestos of politicians and their eventual performance in office. Due to changes in political names and structure, the study could only utilized the manifesto data of the two most popular political parties in Nigeria, from 2007 to 2019. The result of the empirical analysis shows that the model evaluation accuracy stood at 67%. With more adequate data, this result can be improved upon by subsequent research work.

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ΔSCOPE: A new method to quantify 3D biological structures and identify differences in zebrafish forebrain development

Cited by 3 publications

References 40 publications

Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Machine Perception of Political Manifestos in Predicting Performance of Public Office Holders

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