Ω-shaped fiber optic LSPR biosensor based on mismatched hybridization chain reaction and gold nanoparticles for detection of circulating cell-free DNA

Wei, Ning; Zhang, Chuyan; Tian, Ziyi; Luo, Zewei; Hu, Shunming; Qiu, Ren; Li, Yongxin

doi:10.1016/j.bios.2023.115175

Cited by 22 publications

(5 citation statements)

References 30 publications

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“…The sensing behavior agreed with the values estimated for the bulk refractive index sensitivity (RIS) determined for these two systems [56,57], where an almost two-fold increase from 80 ± 4 to 147.7 ± 0.9 nm/RIU was found when Ag was added to Au to form bimetallic nanoparticles. Therefore, as expected, a highly sensitive LSPR-based thin film is required to monitor such functionalization events.…”

Section: Thin Film's Surface Functionalization With Sh-oligonucleotidesupporting

confidence: 83%

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Costa,

Pereira-Silva,

Sousa

et al. 2024

Biosensors

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This work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in a TiO2 dielectric matrix, produced cost-effectively and sustainably by magnetron sputtering. The study focused on the immobilization kinetics of thiolated oligonucleotide probes as biorecognition elements, followed by the evaluation of hybridization events with the target probe. The interaction between the thiolated oligonucleotide probe and the transducer’s surface was assessed by monitoring the LSPR signal with successive additions of probe solution through a microfluidic device. The device was specifically designed and fabricated for this work and adapted to a high-resolution LSPR spectroscopy system with portable characteristics. Benefiting from the synergetic characteristics of Ag and Au in the form of bimetallic nanoparticles, the Au-Ag/TiO2 thin film proved to be more sensitive to thiolated oligonucleotide binding events. Despite the successful surface functionalization with the biorecognition element, the detection of complementary oligonucleotides revealed electrostatic repulsion and steric hindrance, which hindered hybridization with the target oligonucleotide. This study points to an effect that is still poorly described in the literature and affects the design of LSPR biosensors based on nanoplasmonic thin films.

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Section: Thin Film's Surface Functionalization With Sh-oligonucleotidesupporting

confidence: 83%

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Costa,

Pereira-Silva,

Sousa

et al. 2024

Biosensors

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show abstract

“…Moreover, some reported nucleic acid detection strategies (Fig. 11) used for SPR sensors are also applicable to fiber LSPR sensors, including competitive detection of micro ribose nucleic acid (miRNA), sandwich-based detection of ssDNA, and aptamer-based detection, among others [79][80][81][82]. As shown in Fig.…”

Section: Applications Of the Folspr Biosensormentioning

confidence: 99%

“…As shown in Fig. 11(a), in the presence of target cfDNA, the hairpins H 1 -AuNPs and H 2 are triggered by the hybridization chain reaction (HCR), and then the Ω-shaped FOLSPR biosensor fabricated with the capture DNA captures the HCR product for synergistically amplified the LSPR signal [79]. S. Y. Qian et al [82] proposed a phenylboronic acid (PBA) probe for miRNA detection, as shown in Fig.…”

Section: Applications Of the Folspr Biosensormentioning

confidence: 99%

Review of Fiber-Optic Localized Surface Plasmon Resonance Sensors: Geometries, Fabrication Technologies, and Bio-Applications

Lu,

Wang,

Fan

et al. 2024

Photonic Sens

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Localized surface plasmon resonance (LSPR) biosensors, which enable nanoscale confinement and manipulation of light, offer the enhanced sensitivity and electromagnetic energy localization. The integration of LSPR with the fiber-optic technology has led to the development of compact and versatile sensors for miniaturization and remote sensing. This comprehensive review explores various sensor configurations, fiber types, and geometric shapes, highlighting their benefits in terms of sensitivity, integration, and performance improvement. Fabrication techniques such as focused non-chemical bonding strategies and self-assembly of nanoparticles are discussed, providing control over nanostructure morphology and enhancing sensor performance. Bio-applications of fiber-optic LSPR (FOLSPR) sensors are detailed, specifically in biomolecular interactions and analysis of proteins, pathogens and cells, nucleic acids (DNA and RNA), and other small molecules (organic compounds and heavy metal ions). Surface modification and detection schemes are emphasized for their potential for label-free and real-time biosensing. The challenges and prospects of FOLSPR sensors are addressed, including the developments in sensitivity, fabrication techniques, and measurement reliability. Integration with emerging technologies such as nanomaterials is highlighted as a promising direction for future research. Overall, this review provides insights into the advancements and potential applications of FOLSPR sensors, paving the way for sensitive and versatile optical biosensing platforms in various fields.

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“…To circumvent this problem, the functional DNA sequences for amplification should be preferentially linked to the non−sticky end of the hairpins, which will avoid interfering with hybridization. For example, Ning et al modified the poly−A sequence at the non−sticky end of the hairpin probe to connect AuNPs [23], effectively triggering the HCR reaction along with satisfactory products. Similarly, Sun et al possessed an overhang complementary sequence of the aptamer at the 5′ end of the hairpins (non−sticky end) [24], enriching the quantity of the aptamer on the HCR nanowires without affecting the hybridization performance due to the absence of additional strands at the sticky end of the hairpins.…”

Section: Introductionmentioning

confidence: 99%

Improved Catalytic Activity of Spherical Nucleic Acid Enzymes by Hybridization Chain Reaction and Its Application for Sensitive Analysis of Aflatoxin B1

Wang,

Li,

Zeng

et al. 2024

Sensors

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Conventional spherical nucleic acid enzymes (SNAzymes), made with gold nanoparticle (AuNPs) cores and DNA shells, are widely applied in bioanalysis owing to their excellent physicochemical properties. Albeit important, the crowded catalytic units (such as G-quadruplex, G4) on the limited AuNPs surface inevitably influence their catalytic activities. Herin, a hybridization chain reaction (HCR) is employed as a means to expand the quantity and spaces of G4 enzymes for their catalytic ability enhancement. Through systematic investigations, we found that when an incomplete G4 sequence was linked at the sticky ends of the hairpins with split modes (3:1 and 2:2), this would significantly decrease the HCR hybridization capability due to increased steric hindrance. In contrast, the HCR hybridization capability was remarkably enhanced after the complete G4 sequence was directly modified at the non-sticky end of the hairpins, ascribed to the steric hindrance avoided. Accordingly, the improved SNAzymes using HCR were applied for the determination of AFB1 in food samples as a proof-of-concept, which exhibited outstanding performance (detection limit, 0.08 ng/mL). Importantly, our strategy provided a new insight for the catalytic activity improvement in SNAzymes using G4 as a signaling molecule.

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Ω-shaped fiber optic LSPR biosensor based on mismatched hybridization chain reaction and gold nanoparticles for detection of circulating cell-free DNA

Cited by 22 publications

References 30 publications

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Review of Fiber-Optic Localized Surface Plasmon Resonance Sensors: Geometries, Fabrication Technologies, and Bio-Applications

Improved Catalytic Activity of Spherical Nucleic Acid Enzymes by Hybridization Chain Reaction and Its Application for Sensitive Analysis of Aflatoxin B1

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