ФPSII and NPQ to evaluate Bremia lactucae-infection in susceptible and resistant lettuce cultivars

Brabandt, H.; Bauriegel, Elke; Gärber, Ute; Herppich, Werner B.

doi:10.1016/j.scienta.2014.10.012

Cited by 13 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum photochemical efficiency (Fv/Fm) of PSII significantly decreased under BNTZ stress, indicating that the PSII reaction centers was impaired. ΦPSII reflects the actual photochemical efficiency of photosynthetic electron transport, which is the efficiency of PSII light energy capture when the PSII reaction center is closed . ΦPSII also showed a decreasing tendency under BNTZ stress, confirming that the photosynthetic efficiency and accumulated energy for the dark carbon assimilation were negatively affected under the stress of BNTZ.…”

Section: Discussionmentioning

confidence: 89%

“…ΦPSII reflects the actual photochemical efficiency of photosynthetic electron transport, which is the efficiency of PSII light energy capture when the PSII reaction center is closed. 32 ΦPSII also showed a decreasing tendency under BNTZ stress, confirming that the photosynthetic efficiency and accumulated energy for the dark carbon assimilation were negatively affected under the stress of BNTZ. NPQ signifies the situation that the light energy absorbed by the pigment of PSII antenna cannot be properly utilized for photosynthetic electron transport and dissipated in the form of heat.…”

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

Qiao

Chen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Bentazone (BNTZ) is a selective and efficient herbicide used in crop production worldwide. However, the persistence of BNTZ residues in the environment has led to their increasing accumulation in farmland and crops, posing a high risk to human health. To evaluate its impact on crop growth and environmental safety, a comprehensive study was conducted on BNTZ toxicity, metabolic mechanism, and resultant pathways in rice. The rice growth was compromised to the treatment with BNTZ at 0.2–0.8 mg/L (529.95–1060.05 g a.i./ha), while the activities of enzymes including SOD, POD, CAT, GST, GT, and CYP450 were elevated under BNTZ stress. A genome-wide RNA-sequencing (RNA-Seq) was performed to dissect the variation of transcriptomes and metabolic mechanisms in rice exposed to BNTZ. The degradative pathways of BNTZ in rice are involved in glycosylation, hydrolysis, acetylation, and conjugation processes catalyzed by the enzymes. Our data provided evidence that helps understand the BNTZ metabolic and detoxic mechanisms.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: ■ Discussionmentioning

confidence: 99%

Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

Qiao

Chen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…To investigate whether the NbRBP3a protein affects PSII function, we examined TRV‐NbRBP3 silenced plants using a chlorophyll fluorescence video imaging system. The electron transport rate (ETR) (Ghosh et al., 2017 ), actual quantum yield of PSII (Y(II)), redox state of the QA electron acceptor of PSII (1‐qP) and non‐photochemical quenching (NPQ) (Brabandt et al., 2014 ) were used to analyse the photosynthetic activity of different plants infected with pathogens, which elucidated the effect of pathogen infections on PSII activity (de Torres Zabala et al., 2015 ; Littlejohn et al., 2021 ). The results showed that TRV‐NbRBP3 silenced plants displayed a lower maximum quantum yield of PSII (Fv/Fm) compared to the TRV‐GFP silenced plants (Figures 4a and S11 ).…”

Section: Resultsmentioning

confidence: 99%

“…After silencing the NbRBP3a gene in N. benthamiana infected with TRV‐NBRBP3 , the plants had yellow leaves. PSII quantum yield (Y(II)), maximum quantum yield (Fv/Fm), electron transport rate (ETR) and non‐photochemical quenching (NPQ) have been used to analyse photosynthetic activity in different plant species infected with downy mildew and powdery mildew (Brabandt et al., 2014 ). Chlorophyll fluorescence analysis showed that silencing NBRBP3 decreased the activity of PSII and resulted in a decrease in photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…photochemical quenching (NPQ)(Brabandt et al, 2014) were used to analyse the photosynthetic activity of different plants infected with pathogens, which elucidated the effect of pathogen infections on PSII activity(de Torres Zabala et al, 2015;Littlejohn et al, 2021).The results showed that TRV-NbRBP3 silenced plants displayed a lower maximum quantum yield of PSII (Fv/Fm) compared to the TRV-GFP silenced plants (Figures4a and S11). Indeed, the Y(II) and ETR values were much lower in TRV-NbRBP3 silenced plants compared to the TRV-GFP silenced plants (Figure4a).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Phytophthora infestans RXLR effector Pi23014 targets host RNA‐binding protein NbRBP3a to suppress plant immunity

Li,

Liu,

Huang

et al. 2024

Molecular Plant Pathology

View full text Add to dashboard Cite

Phytophthora infestans is a destructive oomycete that causes the late blight of potato and tomato worldwide. It secretes numerous small proteins called effectors in order to manipulate host cell components and suppress plant immunity. Identifying the targets of these effectors is crucial for understanding P. infestans pathogenesis and host plant immunity. In this study, we show that the virulence RXLR effector Pi23014 of P. infestans targets the host nucleus and chloroplasts. By using a liquid chromatogrpahy‐tandem mass spectrometry assay and co‐immunoprecipitation assasys, we show that it interacts with NbRBP3a, a putative glycine‐rich RNA‐binding protein. We confirmed the co‐localization of Pi23014 and NbRBP3a within the nucleus, by using bimolecular fluorescence complementation. Reverse transcription‐quantitative PCR assays showed that the expression of NbRBP3a was induced in Nicotiana benthamiana during P. infestans infection and the expression of marker genes for multiple defence pathways were significantly down‐regulated in NbRBP3‐silenced plants compared with GFP‐silenced plants. Agrobacterium tumefaciens‐mediated transient overexpression of NbRBP3a significantly enhanced plant resistance to P. infestans. Mutations in the N‐terminus RNA recognition motif (RRM) of NbRBP3a abolished its interaction with Pi23014 and eliminated its capability to enhance plant resistance to leaf colonization by P. infestans. We further showed that silencing NbRBP3 reduced photosystem II activity, reduced host photosynthetic efficiency, attenuated Pi23014‐mediated suppression of cell death triggered by P. infestans pathogen‐associated molecular pattern elicitor INF1, and suppressed plant immunity.

show abstract

Flexible Self‐Powered Organic Photodetector with High Detectivity for Continuous On‐Plant Sensing

Schrickx,

Gyurek,

Moore

et al. 2024

Advanced Optical Materials

View full text Add to dashboard Cite

Organic photodetectors (OPDs) exhibit performance on par with inorganic detectors (e.g., Si) but can be ultrathin, ultra‐lightweight, flexible, and mechanically resilient, opening up opportunities for novel applications including optical sensors for continuous human and plant health monitoring. Here, a high‐performance flexible self‐powered OPD designed for on‐plant optical sensing is developed. The OPD employs an electrode consisting of Ag nanowires (NWs) embedded in a UV‐curable resin to achieve a flexible and thin form factor. In addition, the OPD active layer consisting of D18‐Cl and Y6 is sequentially cast to reduce dark current. The flexible OPD is sensitive to 400–950 nm wavelengths and exhibits photodetector characteristics comparable to state‐of‐the‐art rigid OPDs. The responsivity reaches values of 0.47 A W−1 and specific detectivity exceeds 1012 Jones. Owing to the embedded Ag NW electrodes in a thin substrate (t = 20 µm) and sequentially cast active layers, the detector demonstrates excellent bending stability. The photocurrent remains steady across 4000 cycles with a bending radius of 2 mm. The flexible OPD is demonstrated to effectively detect plant uptake of the rare‐earth metal terbium and sense time‐dependent chlorophyll fluorescence. Thus, this work highlights the potential for OPDs as on‐plant sensors to advance precision agriculture.

show abstract

ФPSII and NPQ to evaluate Bremia lactucae-infection in susceptible and resistant lettuce cultivars

Cited by 13 publications

References 34 publications

Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

Multiple Metabolism Pathways of Bentazone Potentially Regulated by Metabolic Enzymes in Rice

Phytophthora infestans RXLR effector Pi23014 targets host RNA‐binding protein NbRBP3a to suppress plant immunity

Flexible Self‐Powered Organic Photodetector with High Detectivity for Continuous On‐Plant Sensing

Contact Info

Product

Resources

About