The spread of farming from western Asia to Europe had profound long-term social and ecological impacts, but identification of the specific nature of Neolithic land management practices and the dietary contribution of early crops has been problematic. Here, we present previously undescribed stable isotope determinations of charred cereals and pulses from 13 Neolithic sites across Europe (dating ca. 5900-2400 cal B.C.), which show that early farmers used livestock manure and water management to enhance crop yields. Intensive manuring inextricably linked plant cultivation and animal herding and contributed to the remarkable resilience of these combined practices across diverse climatic zones. Critically, our findings suggest that commonly applied paleodietary interpretations of human and herbivore δ 15 N values have systematically underestimated the contribution of crop-derived protein to early farmer diets.agriculture | prehistoric | husbandry | paleodiet

Abstract:This paper presents the first study that combines the use of ancient crop and animal stable isotopes (carbon and nitrogen) and Zooarchaeology Mass Spectrometry species identification (ZooMS) for reconstructing early farming practices at Kouphovouno, a Middle-Late Neolithic village in southern Greece (c. 5950-4500 cal. BC). Debate surrounding the nature of early farming predominantly revolves around the intensity of crop cultivation: did early farmers move around the landscape while practicing temporary farming methods such as slash and burn agriculture or did they create more permanent fields by investing high labor inputs into smaller pieces of land that produced higher crop yields? The need to address these questions using a direct assessment of the intensity and scale of cultivation is apparent, and an integrated stable isotope approach provides such an opportunity. The results of this study support the model of small-scale mixed farming, where crop cultivation and animal husbandry are closely integrated. The farmers directed their intensive management towards crops grown for human consumption (free-threshing wheat), while growing fodder crop (hulled barley) more extensively. Pulses were cultivated under a high-manuring/high-watering regime, likely in garden plots in rotation with free-threshing wheat. The diets of the livestock enable us to investigate which parts of the landscape were used for browsing and grazing and indicate that animal management changed in the Late Neolithic. The sheep and goats were now kept in smaller numbers and grazed together and new pasture grasses were sought for the grazing of cattle. This study demonstrates that beyond its applicability for palaeodietary reconstruction, analysis of stable isotopes of archaeological crop and animal remains has important implications for understanding the relationship between humans, plants and animals in an archaeological context.

Though chickens are the most numerous and ubiquitous domestic bird, their origins, the circumstances of their initial association with people, and the routes along which they dispersed across the world remain controversial. In order to establish a robust spatial and temporal framework for their origins and dispersal, we assessed archaeological occurrences and the domestic status of chickens from ∼600 sites in 89 countries by combining zoogeographic, morphological, osteometric, stratigraphic, contextual, iconographic, and textual data. Our results suggest that the first unambiguous domestic chicken bones are found at Neolithic Ban Non Wat in central Thailand dated to ∼1650 to 1250 BCE, and that chickens were not domesticated in the Indian Subcontinent. Chickens did not arrive in Central China, South Asia, or Mesopotamia until the late second millennium BCE, and in Ethiopia and Mediterranean Europe by ∼800 BCE. To investigate the circumstances of their initial domestication, we correlated the temporal spread of rice and millet cultivation with the first appearance of chickens within the range of red junglefowl species. Our results suggest that agricultural practices focused on the production and storage of cereal staples served to draw arboreal red junglefowl into the human niche. Thus, the arrival of rice agriculture may have first facilitated the initiation of the chicken domestication process, and then, following their integration within human communities, allowed for their dispersal across the globe.