This paper examines the distribution of mono- and sesquiterpene
secondary metabolites in 47 plants
present in grazing areas from which cheeses are produced. In
total, 54 terpenoid volatiles, derived
from both highland and lowland plants, were obtained by a gaseous
purge-and-trap system without
the use of organic solvents and then identified by automated GC/MSD-FID
analysis using mass
spectral libraries, retention indices, and data acquired by the authors
with authentic substances.
Single ion monitoring proved valuable in identifying overlapping
compounds with similar spectra
for the subsequent semiquantitative determination of compositions.
These are displayed as radar
plots. It is suggested that flavor/aroma differences in cheeses
produced from animals grazing in
different areas and seasons arise from species (n = 38),
phenological stages (n = 8), locations (n
=
4), and seasonal variation in terpenoids present (the most common were
trans-β-ocimene, limonene,
and trans-β-caryophyllene). This hypothesis is
supported by the fact that distinct variations in
terpenoids with differing odor descriptors and abundances were found.
It is also possible that
terpenes with a higher polarity are less efficiently recovered by the
purge-and-trap method than
compounds of lower polarity. As the radar plots display relative
concentrations based on detection
limits, the relative efficiency of detection for different classes of
compounds needs to be taken into
consideration.
Keywords: GC/MS; flavor; volatile; monoterpene; sesquiterpene; plant;
highland; lowland; pasture;
retention index
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