The Tibet-III air shower array, consisting of 533 scintillation detectors, has been operating successfully at Yangbajing in Tibet, China since 1999. Using the dataset collected by this array from 1999 November through 2005 November, we obtained the energy spectrum of γ-rays from the Crab Nebula, expressed by a power law as (dJ/dE) = (2.09 ± 0.32) × 10 −12 (E/3 TeV) −2.96±0.14 cm −2 s −1 TeV −1 in the energy range of 1.7 to 40 TeV. This result is consistent with other independent γ-ray observations by imaging air Cherenkov telescopes. In this paper, we carefully checked and tuned the performance of the Tibet-III array using data on the moon's shadow in comparison with a detailed Monte Carlo simulation. The shadow is shifted to the west of the moon's apparent position as an effect of the geomagnetic field, although the extent of this displacement depends on the primary energy positively charged cosmic rays. This finding enables us to estimate the systematic error in determining the primary energy from its shower size. This error is estimated to be less than ±12% in our experiment. This energy scale estimation is the first attempt among cosmic-ray experiments at ground level. The systematic pointing error is also estimated to be smaller than 0. • 011. The deficit rate and position of the moon's shadow are shown to be very stable within a statistical error of ±6% year by year. This guarantees the long-term stability of point-like source observation with the Tibet-III array. These systematic errors are adequately taken into account in our study of the Crab Nebula.

AnnongS-1, a thermo-sensitive genic male-sterile (TGMS) rice line, has a new TGMS gene. Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5. In our previous studies based on an F(2) population from the cross between AnnongS-1 and Nanjing11, tms5 was mapped on chromosome 2. Recently, a RIL (recombinant inbred line) population from the same cross was developed and used for the fine mapping of the tms5 gene. Molecular marker techniques combined with BSA (bulked segregant analysis) were used. As a result, two AFLP markers (AF10, AF8), one RAPD marker (RA4), one STS marker (C365-1), one CAPs marker (G227-1) and four SSR markers (RM279, RM492, RM327, RM324) were found to be closely linked to tms5 gene. The DNA sequences of the RFLP marker of C365 and G227 were found in GenBank, and on the basis of these sequences, many primers were designed to amplify the two parents and their RIL population plants. Finally, the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1.04 cM from C365-1 and 2.08 cM from G227-1.

Southern corn rust (SCR), Puccinia polysora Underw, is a destructive disease in maize ( Zea mays L.). Inbred line Qi319 is highly resistant to SCR. Results from the inoculation test and genetic analysis of SCR in five F(2) populations and five BC(1)F(1 )populations derived from resistant parent Qi319 clearly indicate that the resistance to SCR in Qi319 is controlled by a single dominant resistant gene, which was named RppQ. Simple sequence repeat (SSR) analysis was carried out in an F(2) population derived from the cross "Qi319x340". Twenty SSR primer pairs evenly distributed on chromosome10 were screened at first. Out of them, two primer pairs, phi118 and phi 041, showed linkage with SCR resistance. Based on this result, eight new SSR primer pairs surrounding the region of primers phi118 and phi 041 were selected and further tested regarding their linkage relation with RppQ. Results indicated that SSR markers umc1,318 and umc 2,018 were linked to RppQ with a genetic distance of 4.76 and 14.59 cM, respectively. On the other side of RppQ, beyond SSR markers phi 041 and phi118, another SSR marker umc1,293 was linked to RppQ with a genetic distance of 3.78 cM. Because the five linkage SSR markers (phi118, phi 041, umc1,318, umc 2,018 and umc1,293) are all located on chromosome 10, the RppQ gene should also be located on chromosome 10. In order to fine map the RppQ gene, AFLP (amplified fragment length polymorphism) analysis was carried out. A total 54 AFLP primer combinations were analyzed; one AFLP marker, AF1, from the amplification products of primer combination E-AGC/M-CAA, showed linkage with the RppQ gene in a genetic distance of 3.34 cM. Finally the RppQ gene was mapped on the short arm of chromosome 10 between SSR markers phi 041 and AFLP marker AF1 with a genetic distance of 2.45 and 3.34 cM respectively.