The use of reclaimed wastewater (RWW) for the irrigation of crops may result in the continuous exposure of the agricultural environment to antibiotics, antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In recent years, certain evidence indicate that antibiotics and resistance genes may become disseminated in agricultural soils as a result of the amendment with manure and biosolids and irrigation with RWW. Antibiotic residues and other contaminants may undergo sorption/desorption and transformation processes (both biotic and abiotic), and have the potential to affect the soil microbiota. Antibiotics found in the soil pore water (bioavailable fraction) as a result of RWW irrigation may be taken up by crop plants, bioaccumulate within plant tissues and subsequently enter the food webs; potentially resulting in detrimental public health implications. It can be also hypothesized that ARGs can spread among soil and plant-associated bacteria, a fact that may have serious human health implications. The majority of studies dealing with these environmental and social challenges related with the use of RWW for irrigation were conducted under laboratory or using, somehow, controlled conditions. This critical review discusses the state of the art on the fate of antibiotics, ARB and ARGs in agricultural environment where RWW is applied for irrigation. The implications associated with the uptake of antibiotics by plants (uptake mechanisms) and the potential risks to public health are highlighted. Additionally, knowledge gaps as well as challenges and opportunities are addressed, with the aim of boosting future research towards an enhanced understanding of the fate and implications of these contaminants of emerging concern in the agricultural environment. These are key issues in a world where the increasing water scarcity and the continuous appeal of circular economy demand answers for a long-term safe use of RWW for irrigation.

Wastewater (WW) reuse for vegetable crops irrigation is regularly applied worldwide. Such a practice has been found to allow the uptake of pharmaceutical active compounds (PhACs) by plants and their subsequent entrance to the food web, representing an important alternative pathway for the exposure of humans to PhACs, with potential health implications. Herein we report the impacts of the long-term (three consecutive years) WW irrigation of a tomato crop with two differently treated effluents under real agricultural conditions, on (1) the soil concentration of selected PhACs (i.e. diclofenac, DCF; sulfamethoxazole, SMX; trimethoprim, TMP), (2) the bioaccumulation of these PhACs in tomato fruits, and (3) the human risks associated with the consumption of WW-irrigated fruits. Results revealed that the concentration of the studied PhACs in both the soil and tomato fruits varied depending on the qualitative characteristics of the treated effluent applied and the duration of WW irrigation. The PhAC with the highest soil concentration throughout the studied period was SMX (0.98 μg kg), followed by TMP (0.62 μg kg) and DCF (0.35 μg kg). DCF was not found in tomato fruits harvested from WW-irrigated plants during the first year of the study. However, DCF displayed the highest fruit concentration (11.63 μg kg) throughout the study (as a result of prolonged WW irrigation), followed by SMX (5.26 μg kg) and TMP (3.40 μg kg). The calculated fruit bioconcentration factors (BCF) were extremely high for DCF in the 2nd (108) and 3rd year (132) of the experimental period, with the respective values for SMX (0.5-5.4) and TMP (0.2-6.4) being significantly lower. The estimated threshold of toxicity concern (TTC) and hazard quotients (HQ) values revealed that the consumption of fruits harvested from tomato plants irrigated for long period with the WW applied for irrigation under field conditions in this study represent a de minimis risk to human health. However, more studies need to be performed in order to obtain more solid information on the safety of WW reuse for irrigation.

The reuse of treated wastewater (TWW) for irrigation and the use of biosolids and manures as soil amendment constitute significant pathways for the introduction of the contaminants of emerging concern (CECs) to the agricultural environment. Consequently, CECs are routinely detected in TWW-irrigated agricultural soils and runoff from such sites, in biosolids-and manure-amended soils, and in surface and groundwater systems and sediments receiving TWW. Crop plants grown in such contaminated agricultural environments have been found to uptake and accumulate CECs in their tissues, constituting possible vectors of introducing CECs into the food chain; an issue that is presently considered of high priority, thus needing intensive investigation. This review paper aims at highlighting the responsible mechanisms for the uptake of CECs by plants and the ability of each crop plant species to uptake and accumulate CECs in its edible tissues, thus providing tools for mitigating the introduction of these contaminants into the food chain. Both biotic (e.g. plants' genotype and physiological state, soil fauna) and abiotic factors (e.g. soil pore water chemistry, physico-chemical properties of CECs, environmental perturbations) have been proven to influence the ability of crop plants to uptake and accumulate CECs. According to authors' estimates, based on the thorough elaboration of knowledge produced by existing relevant studies, the ability of crop plants to uptake and accumulate CECs decrease in the order of leafy vegetables > root vegetables > cereals and fodder crops > fruit vegetables; though, the uptake of CECs by important crop plants, such as fruit trees, is not yet evaluated. Overall, further studies must be performed to estimate the potential of crop plants to uptake and accumulate CECs in their edible tissues, and to characterize the risk for human health represented by their presence in human and livestock food products.

Pharmaceutically active compounds (PhACs) have been recently shown to exert phytotoxic effects. The present study explores the uptake, systemic translocation, and abiotic stress responses and detoxification mechanisms induced by the exposure of alfalfa plants grown in sand under greenhouse conditions to four common, individually applied PhACs (10μgL(-1)) (diclofenac, sulfamethoxazole, trimethoprim, 17a-ethinylestradiol) and their mixture. Stress physiology markers (lipid peroxidation, proline, H2O2 and NO content, antioxidant activity assays) and gene expression levels of key plant detoxification components (including glutathione S-transferases, GST7, GST17; superoxide dismutases, CuZnSOD, FeSOD; proton pump, H(+)-ATP, and cytochrome c oxidase, CytcOx), were evaluated. PhACs were detected in significantly higher concentrations in roots compared with leaves. Stress related effects, manifested via membrane lipid peroxidation and oxidative burst, were local (roots) rather than systemic (leaves), and exacerbated when the tested PhACs were applied in mixture. Systemic accumulation of H2O2 in leaves suggests its involvement in signal transduction and detoxification responses. Increased antioxidant enzymatic activities, as well as upregulated transcript levels of GST7, GST17, H(+)-ATPase and CytcOx, propose their role in the detoxification of the selected PhACs in plants. The current findings provide novel biochemical and molecular evidence highlighting the studied PhACs as an emerging abiotic stress factor, and point the need for further research on wastewater flows under natural agricultural environments.

Advanced tertiary treatment and disinfection technologies have enabled the production of wastewater (WW) with quality complying with the established criteria for reuse in agriculture. This study assessed the impacts of tomato crop irrigation with two qualitatively distinct treated WW effluents, as compared to control tubewell water (TW) irrigation, on the soil geochemical properties, tomato fruit safety and crop productivity. The treated effluents reused for irrigation were produced in two Municipal Wastewater Treatment Plants (MWTPs) utilizing two discrete tertiary treatment and disinfection technologies, i.e. Slow Sand Filtration and chlorination (MWTP I), and Membrane Bioreactor and UV radiation (MWTP II), respectively. The impacts on soil pH, electrical conductivity, total organic C, Cl − , NO 3 − and heavy metal (Zn, Mn, Ni, Cu, Co) content were evaluated. In addition, the heavy metal content in tomato fruits and leaves, as well as the microbial load in fruit flesh and peel was determined. Crop productivity was measured by the mean fruit weight and maximum diameter, and by the number of fruits per harvest. Irrigation with either WW did not significantly affect the soil pH, organic C and heavy metal content, as well as crop productivity, in comparison to control TW irrigation. Furthermore, the heavy metal content of tomato fruits and leaves in all irrigation treatments was found to be below the maximum permissible levels set for fruit safety and the critical tissue concentration for phytotoxicity, respectively. Moreover, no microbiological contamination (total coliform, fecal coliform, Escherichia coli, Salmonella spp., Listeria spp.) of tomato fruits was found from any irrigation treatment. Overall, results obtained with regard to the parameters examined strongly suggest that advanced tertiary treated effluent of good quality might be safely reused, in terms of both environmental sustainability and public health safety, for vegetable irrigation, concurrently promoting water use efficiency in dry areas.