Circulating leukocytes can be used as an effective model to understand the heat stress response of different cattle types and buffaloes. This investigation aimed to determine the temporal profile of HSPs (HSP40, HSP60, HSP70, and HSP90) expression in circulating peripheral blood mononuclear cells (PBMCs) of Murrah buffaloes, Holstein-Friesian (HF), and Sahiwal cows in response to sublethal heat shock at 42°C. The viability data indicated HF PBMCs to be the most affected to the heat shock, whereas Sahiwal PBMCs were least affected, indicating its better survivability during the heat stress condition. The qRT-PCR expression data showed significant increase in mRNA expression of the analyzed HSPs genes after heat stimuli to the PBMCs under in vitro condition. In each case, the HSPs were most upregulated at 2 h after the heat stress. Among the HSPs, HSP70 was relatively more expressed followed by HSP60 indicating the action of molecular chaperones to stabilize the native conformation of proteins. However, PBMCs from different cattle types and buffaloes showed difference in the extent of transcriptional response. The level of expression of HSPs throughout the time period of heat stress was highest in buffaloes, followed by HF and Sahiwal cows. The higher abundance of HSP70 mRNA at each time point after heat stress showed prolonged effect of heat stress in HF PBMCs. The data presented here provided initial evidence of transcriptional differences in PBMCs of different cattle types and buffaloes and warrant further research.

This study analysed buffaloes from north-east India and compared their nuclear and mitochondrial DNA variations with buffaloes of mainland India, China, Mediterranean and South-East Asia. Microsatellite genotypes of 338 buffaloes including 210 from six north-east Indian buffalo populations and three mainland Indian breeds were analysed to evaluate their genetic structure and evolutionary relationships. Phylogenetic analysis and multidimensional scaling plot of pairwise FST revealed the clustering of all swamp-type buffaloes of north-east India with Lower Assamese (significantly hybrid type) buffaloes in one plane and all the mainland river buffaloes in another plane while the upper Assamese buffaloes being distinct from both these clusters. Analysis of mtDNA D-loop region of 530-bp length was performed on 345 sequences belonging to 23 buffalo populations from various geographical regions to establish the phylogeography of Indian water buffalo. The swamp buffaloes of north-east India clustered with both the lineages of Chinese swamp buffalo. Multidimensional scaling display of pairwise FST derived from mitochondrial DNA data showed clustering of upper Assamese, Chilika and Mediterranean buffaloes distinctly from all the other Indian buffalo populations. Median-joining network analysis further confirmed the distinctness and ancestral nature of these buffaloes. The study revealed north-east region of India forming part of the wider hybrid zone of water buffalo that may probably extend from north-east India to South-East Asia.

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SummaryThis study was carried out on 2 309 households in 60 villages with the objective of documenting the performance of Mecheri sheep in their native environment. The study revealed that Mecheri sheep were distributed in Salem, Erode, Karur, Namakkal and parts of Dharmapuri districts of Tamil Nadu. This tract lies in the north-western agro-climatic zone of Tamil Nadu and the climate is generally hot, semi-arid and tropical in nature.The average flock size of Mecheri sheep was 24, of which, there were 1 ram, 17 ewes and 6 lambs. Mecheri sheep of medium size with a compact body and covered with short hairs, which are not shorn. They are light brown in colour. Their profile reveals a slightly Roman nose. Both males and females are polled. The pooled means for height at withers, chest girth and body length at above 24 months of age were 67±0.4, 74±0.4 and 66±0.4 cm, respectively. The body weight of the animals at birth 3, 6, 9 and 12 months of age was 2.82±0.01, 10.9±0.1, 15.6±0.1, 17.6±0.2 and 21.1±0.1, kg respectively. The dressing percentage of males and females was 54.4±0.4 and 51.8±0.5, respectively. Average ages at first oestrus, mating and lambing were 12.1±0.1, 12.4±0.1 and 17.4±0.1 months, respectively. The average lambing interval was 226±0.7 days and the average litter size was one.

Six morphometric traits (height at withers, body length, chest girth, ear length, tail length and body weight) were analyzed to characterize from a breed point of view 1981 sheep from four ovine breeds (Bellary, Kenguri, Hassan and Mandya) of southern peninsular zone of India. Discriminant Function Analysis was used to distinguish between four breeds by morphometric traits. The population variability showed Kenguri ewes were the largest and heaviest followed by Bellary, Hassan and Mandya whereas Kenguri rams were followed by Bellary, Mandya and Hassan. Overall sexual dimorphism (m/f) was 1.13, with Kenguri males being 47% heavier than females. The coefficient of variation of all traits in four breeds ranged from 4.06% to 30.28%. The flocks and age effects showed a high heterogeneity among females of different flocks. Height at withers was most discriminating trait in separating the four sheep breeds. The Mahalanobis distance of the morphological traits between Kenguri and Mandya sheep was most while the least differentiation was observed between Kenguri and Bellary sheep. Nearest neighbour discriminant analysis showed that most Kenguri sheep were classified into their source population followed by Mandya. However, varied percentages of misclassification between different breeds were observed showing the level of genetic exchange that has taken place between the breeds overtime. UPGMA based dendrogram showed formation of two separate groups; Mandya and Hassan clustered together while Bellary and Kenguri formed other group.