Introduction of exogenous DNA into mammalian cells represents a powerful approach for manipulating signal transduction. The available techniques, however, are limited by low transduction efficiency and low cell viability after transduction. Here we report a highly efficient molecular delivery technique, named nanotube spearing, based on the penetration of nickel-embedded nanotubes into cell membranes by magnetic field driving. DNA plasmids containing the enhanced green fluorescent protein (EGFP) sequence were immobilized onto the nanotubes, and subsequently speared into targeted cells. We have achieved an unprecedented high transduction efficiency in Bal17 B-lymphoma, ex vivo B cells and primary neurons with high viability after transduction. This technique may provide a powerful tool for highly efficient gene transfer into a variety of cells, especially the hard-to-transfect cells.
A transparent, conductive, and flexible electrode is demonstrated. It is based on an inexpensive and easily manufacturable metallic network formed by depositing metals onto a template film. This electrode shows excellent electro-optical properties, with the figure of merit ranging from 300 to 700, and transmittance from 82% (~4.3 Ω sq(-1) ) to 45% (~0.5 Ω sq(-1) ).
We demonstrate here that large area periodic arrays of well-aligned carbon nanotubes can be fabricated inexpensively on Ni dots made by the
process of self-assembly nanosphere lithography. These periodic arrays appear colorful due to their efficient reflection and diffraction of
visible light. In addition, due to their honeycomb lattice structure, these arrays can act as photonic band gap crystals in the visible frequency
range. In this report, we present the initial exploration of the optical properties of such arrays. Here we show that these potential 2D photonic
band gap crystal arrays might find very important applications in optoelectronics.
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