Much remains to be learned about how anthropogenic structures affect migration in large mammals. We examined the potential influence of the international railroad in Mongolia on migration in Mongolian gazelles (Procapra gutturosa). We examined gazelle movements and the normalized difference vegetation index (NDVI) on both sides of the railroad. The tracked gazelles never crossed the railroad, despite movements that mainly followed the railroad in winter and higher NDVI values on the opposite side. It is likely that the railroad had a barrier effect on gazelle migration because it split the gazelles' habitat. Our results, although based on an extremely small sample, have utility in indicating approaches that might be useful for examining migration in ungulates.Resumen: Falta mucho por conocer sobre el efecto de estructuras antropogénicas sobre la migración de mamíferos mayores. Examinamos la potencial influencia del ferrocarril internacional en Mongolia sobre la migración de gacelas de Mongolia ( Procapra gutturosa). Examinamos los movimientos de las gacelas y eĺ ındice de vegetación de diferencia normalizada (IVDN) a ambos lados de la vía férrea. Las gacelas rastreadas nunca cruzaron la vía férrea, a pesar de que los movimientos mayormente seguían la vía en invierno y que los valores de IVDN eran mayores del lado contrario. Es probable que la vía férrea produjera un efecto de barrera sobre la migración de las gacelas porque dividió al hábitat de las gacelas. Nuestros resultados, aunque basados en una muestra extremadamente pequeña, sonútiles para señalar estrategias que pueden ser utilizadas provechosamente para examinar la migración de ungulados.

Conservation and management are urgently required for Mongolian gazelles Procapra gutturosa inhabiting the Mongolian steppe. We captured and satellitecollared two adult females in Dornogobi Province and two adult females in Omnogobi Province and examined whether their seasonal migration corresponded to shifts in the normalized difference vegetation index (NDVI) in their habitat. The mean NDVI values of their annual, summer and winter ranges were calculated based on data acquired by the moderate-resolution imaging spectrometer (MODIS) onboard the Terra satellite. Satellite tracking of gazelles proved their ability to move long distances and provided details of their migration routes. In Omnogobi, the NDVI of the summer range was higher than those of annual and winter ranges during summer, but from October to November the NDVI of the summer range was lower than annual or winter ranges. The shift in NDVI values between summer and winter ranges corresponded with seasonal migrations of gazelles. In contrast, NDVI values were higher in the winter ranges than in both the summer and annual ranges throughout the year in Dornogobi. The results showed that the NDVI is a good indicator of gazelle habitat, but the NDVI alone cannot the explain seasonal migration of gazelles. It is important to evaluate the effectiveness and limitations of the NDVI as an indicator of habitat quality.

Hybridization between wild species and their domestic congeners often threatens the gene pool of the wild species. The last wild Bactrian camel (Camelus ferus) populations in Mongolia and China are examples of populations facing such a hybridization threat. To address this key issue in the conservation of wild camels, we analysed wild, hybrid and domestic Bactrian camels (Camelus bactrianus) originating from Mongolia, China and Austria. Through screening of an 804-base-pair mitochondrial fragment, we identified eight mitochondrial haplotypes and found high sequence divergence (1.9%) between C. ferus and C. bactrianus. On the basis of a mitochondrial DNA sequence fixed difference, we developed a diagnostic PCR restriction fragment length polymorphism (PCR-RFLP) assay to differentiate between wild and domestic camel samples. We applied the assay to 81 individuals and confirmed the origin of all samples including five hybrids with known maternal ancestry. The PCR-RFLP system was effective for both traditional (blood, skin) and non-invasive samples (faeces, hair), as well as for museum specimens. Our results demonstrate high levels of mitochondrial differentiation between wild and domestic Bactrian camels and that maternal hybridization can be detected by a rapid and reliable PCR-RFLP system.

This article documents the addition of 205 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Bagassa guianensis, Bulweria bulwerii, Camelus bactrianus, Chaenogobius annularis, Creontiades dilutus, Diachasmimorpha tryoni, Dioscorea alata, Euhrychiopsis lecontei, Gmelina arborea, Haliotis discus hannai, Hirtella physophora, Melanaphis sacchari, Munida isos, Thaumastocoris peregrinus and Tuberolachnus salignus. These loci were cross-tested on the following species: Halobaena caerulea, Procellaria aequinoctialis, Oceanodroma monteiroi, Camelus ferus, Creontiades pacificus, Dioscorea rotundata, Dioscorea praehensilis, Dioscorea abyssinica, Dioscorea nummularia, Dioscorea transversa, Dioscorea esculenta, Dioscorea pentaphylla, Dioscorea trifida, Hirtella bicornis, Hirtella glandulosa, Licania alba, Licania canescens, Licania membranaceae, Couepia guianensis and 7 undescribed Thaumastocoris species.

HighlightsWild Bactrian camels persist in some of the most remote desert locations in northern China and southern Mongolia.Individual annual home ranges in the Mongolian Gobi were > 12,000 km2 and average straight line distances 3.0-6.4 km/day.Wild camels preferred intermediate productivity values and landscape parameters, but an avoided steep slopes.Wild camels still roam the entire Great Gobi A Protected Area, are highly mobile, and very sensitive to human disturbance.More data from additional wild camels as a foundation for evidence driven conservation actions is urgently needed.