This study examined low-frequency ultrasonic vocalizations (lUSVs) in rats during two types of sexual interactions: postejaculatory interval (PEI) and barrier-noncontact (NC) test. We report distinct classes of lUSVs that can be assigned to different emotional states; relaxation vs. frustration. Totally flat, 22-kHz calls (Class A), were observed during the relaxation state following ejaculation; characterized by immobilization or grooming during the PEI. On the other hand, two-three component lUSVs (Class B) that start at a higher frequency (45-kHz: flat, upward or short signal) and then shift to 35-23-kHz (mostly to 28-23-kHz), correspond as we assume, to arousal and frustration-active states associated with sniffing a hole or exploration during the NC test. We suggest that momentary, abrupt decreases of arousal during the frustration state correspond to Class B lUSVs. The detailed spectral analysis of the high-frequency component of two-component lUSVs is crucial for establishing the relationship between such lUSVs and the corresponding behavior and emotional states. Our studies indicate that while the two-component Class B 22-kHz lUSVs may relate to the frustration state, a single component, flat, Class A lUSV relates to the relaxation state. The results of these studies support a notion that rats emit distinct vocalization patterns, reflecting their emotional states.

It has been assumed that the 22-kHz ultrasonic vocalizations (USVs) are emitted by adult rats as a result of a negative emotional state. However, emission of the 22-kHz vocalizations by male rats has been also observed following ejaculation, which has a high rewarding value as shown by a conditioned place preference test. These observations suggest that 22-kHz USVs may also occur in response to a positive emotional state. The aim of this study was to determine whether the postejaculatory 22-kHz USVs are related to conditioning processes. The 22 kHz USVs were recorded in Sprague-Dawley males in the postejaculatory refractory period during conditioning processes to a new chamber unrelated to copulation. During the first session in the clean recording chamber, males vocalized marginally and exhibited intensive rearing behavior. From the second to fourth sessions, vocalization duration increased and the number of rearing decreased. Following established conditioning process, odor cues from foreign males, but not the familiar ones, resulted in decreased duration of 22-kHz USVs and increased the number of rearing. On the other hand, in the presence of mating cues (copulatory chamber and presence of the female), males exhibited increased duration of postejaculatory 22-kHz USVs and reduced number of rearing. These results demonstrated that the conditioning to the cues, both unrelated and related to copulation, is important for evoking postejaculatory 22-kHz USVs as well as the relaxation state. Furthermore, these results confirmed the postejaculatory 22-kHz USVs' involvement in expression of the positive emotional state. (PsycINFO Database Record

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